A droplet-based microfluidic chip as a platform for leukemia cell lysate identification using surface-enhanced Raman scattering

A new approach is presented for cell lysate identification which uses SERS-active silver nanoparticles and a droplet-based microfluidic chip. Eighty-nanoliter droplets are generated by injecting silver nanoparticles, KCl as aggregation agent, and cell lysate containing cell constituents, such as nuc...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2018-01, Vol.410 (3), p.999-1006
Hauptverfasser:	Hassoun, Mohamed, Rüger, Jan, Kirchberger-Tolstik, Tatiana, Schie, Iwan W., Henkel, Thomas, Weber, Karina, Cialla-May, Dana, Krafft, Christoph, Popp, Jürgen
Format:	Artikel
Sprache:	eng
Schlagworte:	ABCs 16th Anniversary Analytical Chemistry Biochemistry Cancer cells Cancer diagnosis Carbohydrates Cell constituents Cell Line, Tumor Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Continuous flow Diagnosis Droplets Equipment Design Food Science Health aspects Humans Laboratory Medicine Leukemia Leukemia - diagnosis Lysates Metabolites Metal Nanoparticles - chemistry Methods Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Mineral oils Monitoring/Environmental Analysis Nanoparticles Nucleic acids Potassium chloride Raman spectra Raman spectroscopy Reproducibility Research Paper Silver Silver - chemistry Sonication Spectral sensitivity Spectrum analysis Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods Support vector machines Technology application Tumor cell lines
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