MS/MS-Free Protein Identification in Complex Mixtures Using Multiple Enzymes with Complementary Specificity

In this work, we present the results of evaluation of a workflow that employs a multienzyme digestion strategy for MS1-based protein identification in “shotgun” proteomic applications. In the proposed strategy, several cleavage reagents of different specificity were used for parallel digestion of th...

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Veröffentlicht in:	Journal of proteome research 2017-11, Vol.16 (11), p.3989-3999
Hauptverfasser:	Ivanov, Mark V, Tarasova, Irina A, Levitsky, Lev I, Solovyeva, Elizaveta M, Pridatchenko, Marina L, Lobas, Anna A, Bubis, Julia A, Gorshkov, Mikhail V
Format:	Artikel
Sprache:	eng
Schlagworte:	Complex Mixtures - analysis Enzymes - metabolism HeLa Cells Humans Proteins - metabolism Proteomics - methods Tandem Mass Spectrometry - methods
Online-Zugang:	Volltext
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