Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells
Section of Neurobiology, Physiology and Behavior, Division of Biological Sciences, University of California, Davis, California Submitted 10 June 2005; accepted in final form 17 August 2005 The Ca 2+ that promotes transmitter release is generally thought to enter presynaptic terminals through voltage...
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| Veröffentlicht in: | Journal of neurophysiology 2005-12, Vol.94 (6), p.4196-4208 |
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| container_title | Journal of neurophysiology |
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| creator | Warrier, Ajithkumar Borges, Salvador Dalcino, David Walters, Cameron Wilson, Martin |
| description | Section of Neurobiology, Physiology and Behavior, Division of Biological Sciences, University of California, Davis, California
Submitted 10 June 2005;
accepted in final form 17 August 2005
The Ca 2+ that promotes transmitter release is generally thought to enter presynaptic terminals through voltage-gated Ca 2+ channels. Using electrophysiology and Ca 2+ imaging, we show that, in amacrine cell dendrites, at least some of the Ca 2+ that triggers transmitter release comes from endoplasmic reticulum Ca 2+ stores. We show that both inositol 1,4,5-trisphosphate receptors (IP 3 Rs) and ryanodine receptors (RyRs) are present in these dendrites and both participate in the elevation of cytoplasmic [Ca 2+ ] during the brief depolarization of a dendrite. Only the Ca 2+ released through IP 3 Rs, however, seems to promote the release of transmitter. Antagonists for the IP 3 R reduced transmitter release, whereas RyR blockers had no effect. Application of an agonist for metabotropic glutamate receptor, known to liberate Ca 2+ from internal stores, enhanced both spontaneous and evoked transmitter release.
Address for reprint requests and other correspondence: M. Wilson, Section of Neurobiology, Physiology and Behavior, Div. of Biological Sciences, UC Davis, Davis, CA 95616 (E-mail: mcwilson{at}ucdavis.edu ) |
| doi_str_mv | 10.1152/jn.00604.2005 |
| format | Article |
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Submitted 10 June 2005;
accepted in final form 17 August 2005
The Ca 2+ that promotes transmitter release is generally thought to enter presynaptic terminals through voltage-gated Ca 2+ channels. Using electrophysiology and Ca 2+ imaging, we show that, in amacrine cell dendrites, at least some of the Ca 2+ that triggers transmitter release comes from endoplasmic reticulum Ca 2+ stores. We show that both inositol 1,4,5-trisphosphate receptors (IP 3 Rs) and ryanodine receptors (RyRs) are present in these dendrites and both participate in the elevation of cytoplasmic [Ca 2+ ] during the brief depolarization of a dendrite. Only the Ca 2+ released through IP 3 Rs, however, seems to promote the release of transmitter. Antagonists for the IP 3 R reduced transmitter release, whereas RyR blockers had no effect. Application of an agonist for metabotropic glutamate receptor, known to liberate Ca 2+ from internal stores, enhanced both spontaneous and evoked transmitter release.
Address for reprint requests and other correspondence: M. Wilson, Section of Neurobiology, Physiology and Behavior, Div. of Biological Sciences, UC Davis, Davis, CA 95616 (E-mail: mcwilson{at}ucdavis.edu )</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.00604.2005</identifier><identifier>PMID: 16293593</identifier><language>eng</language><publisher>United States: Am Phys Soc</publisher><subject>Amacrine Cells - drug effects ; Amacrine Cells - metabolism ; Animals ; Boron Compounds - pharmacology ; Caffeine - pharmacology ; Calcium - metabolism ; Calcium Channels - metabolism ; Calcium-Binding Proteins - metabolism ; Central Nervous System Stimulants - pharmacology ; Chick Embryo ; Diagnostic Imaging - methods ; Dose-Response Relationship, Drug ; Drug Interactions ; Electric Stimulation - methods ; Endoplasmic Reticulum - drug effects ; Endoplasmic Reticulum - metabolism ; Evoked Potentials - drug effects ; Evoked Potentials - physiology ; Evoked Potentials - radiation effects ; Excitatory Amino Acid Agonists - pharmacology ; gamma-Aminobutyric Acid - metabolism ; gamma-Aminobutyric Acid - pharmacology ; Glycine - analogs & derivatives ; Glycine - pharmacology ; Heparin - pharmacology ; Immunohistochemistry - methods ; Inositol 1,4,5-Trisphosphate Receptors ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Membrane Potentials - radiation effects ; Microscopy, Confocal - methods ; Organic Chemicals - metabolism ; Patch-Clamp Techniques - methods ; Phenylacetates - pharmacology ; Receptors, Cytoplasmic and Nuclear - metabolism ; Retina - cytology ; Ryanodine - pharmacology ; Ryanodine Receptor Calcium Release Channel - metabolism ; Time Factors</subject><ispartof>Journal of neurophysiology, 2005-12, Vol.94 (6), p.4196-4208</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-751e1601146284200c02d39c5eea2ec01b326f1dccce5d75cd596735807603703</citedby><cites>FETCH-LOGICAL-c398t-751e1601146284200c02d39c5eea2ec01b326f1dccce5d75cd596735807603703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16293593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Warrier, Ajithkumar</creatorcontrib><creatorcontrib>Borges, Salvador</creatorcontrib><creatorcontrib>Dalcino, David</creatorcontrib><creatorcontrib>Walters, Cameron</creatorcontrib><creatorcontrib>Wilson, Martin</creatorcontrib><title>Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description>Section of Neurobiology, Physiology and Behavior, Division of Biological Sciences, University of California, Davis, California
Submitted 10 June 2005;
accepted in final form 17 August 2005
The Ca 2+ that promotes transmitter release is generally thought to enter presynaptic terminals through voltage-gated Ca 2+ channels. Using electrophysiology and Ca 2+ imaging, we show that, in amacrine cell dendrites, at least some of the Ca 2+ that triggers transmitter release comes from endoplasmic reticulum Ca 2+ stores. We show that both inositol 1,4,5-trisphosphate receptors (IP 3 Rs) and ryanodine receptors (RyRs) are present in these dendrites and both participate in the elevation of cytoplasmic [Ca 2+ ] during the brief depolarization of a dendrite. Only the Ca 2+ released through IP 3 Rs, however, seems to promote the release of transmitter. Antagonists for the IP 3 R reduced transmitter release, whereas RyR blockers had no effect. Application of an agonist for metabotropic glutamate receptor, known to liberate Ca 2+ from internal stores, enhanced both spontaneous and evoked transmitter release.
Address for reprint requests and other correspondence: M. Wilson, Section of Neurobiology, Physiology and Behavior, Div. of Biological Sciences, UC Davis, Davis, CA 95616 (E-mail: mcwilson{at}ucdavis.edu )</description><subject>Amacrine Cells - drug effects</subject><subject>Amacrine Cells - metabolism</subject><subject>Animals</subject><subject>Boron Compounds - pharmacology</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Central Nervous System Stimulants - pharmacology</subject><subject>Chick Embryo</subject><subject>Diagnostic Imaging - methods</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Interactions</subject><subject>Electric Stimulation - methods</subject><subject>Endoplasmic Reticulum - drug effects</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Evoked Potentials - drug effects</subject><subject>Evoked Potentials - physiology</subject><subject>Evoked Potentials - radiation effects</subject><subject>Excitatory Amino Acid Agonists - pharmacology</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - pharmacology</subject><subject>Heparin - pharmacology</subject><subject>Immunohistochemistry - methods</subject><subject>Inositol 1,4,5-Trisphosphate Receptors</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Membrane Potentials - radiation effects</subject><subject>Microscopy, Confocal - methods</subject><subject>Organic Chemicals - metabolism</subject><subject>Patch-Clamp Techniques - methods</subject><subject>Phenylacetates - pharmacology</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Retina - cytology</subject><subject>Ryanodine - pharmacology</subject><subject>Ryanodine Receptor Calcium Release Channel - metabolism</subject><subject>Time Factors</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MFv2jAUBnBr6lQo63HXKqfuFPpsx3Z8ZGhQpKqTGDtbwXmAkZNQO1HHf79koO20ky3r9z4_fYR8pjClVLCnYz0FkJBNGYD4QMb9G0up0PkNGQP0dw5KjchdjEcAUALYLRlRyTQXmo_J67zw1nVVsghNlazqFkNd-ORH2wSMySa4_R5DTJazr7NkjR6LiBe6xtYNclYVNrgakzl6Hz-Rj7vCR7y_nhPyc_FtM39OX74vV_PZS2q5zttUCYpUAqWZZHnWb26BlVxbgVgwtEC3nMkdLa21KEolbCm0VFzkoCRwBXxCHi-5p9C8dRhbU7lo-w2KGpsuGqq5ymkue5heoA1NjAF35hRcVYSzoWCGAs2xNn8KNEOBvX-4BnfbCst_-tpYD75cwMHtD-8uoDkdztE1vtmfhyydGWkyqoev-f_lovN-g7_afuTvhDmVO_4bKBuJrw</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Warrier, Ajithkumar</creator><creator>Borges, Salvador</creator><creator>Dalcino, David</creator><creator>Walters, Cameron</creator><creator>Wilson, Martin</creator><general>Am Phys Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope></search><sort><creationdate>20051201</creationdate><title>Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells</title><author>Warrier, Ajithkumar ; Borges, Salvador ; Dalcino, David ; Walters, Cameron ; Wilson, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-751e1601146284200c02d39c5eea2ec01b326f1dccce5d75cd596735807603703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amacrine Cells - drug effects</topic><topic>Amacrine Cells - metabolism</topic><topic>Animals</topic><topic>Boron Compounds - pharmacology</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Central Nervous System Stimulants - pharmacology</topic><topic>Chick Embryo</topic><topic>Diagnostic Imaging - methods</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Interactions</topic><topic>Electric Stimulation - methods</topic><topic>Endoplasmic Reticulum - drug effects</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Evoked Potentials - drug effects</topic><topic>Evoked Potentials - physiology</topic><topic>Evoked Potentials - radiation effects</topic><topic>Excitatory Amino Acid Agonists - pharmacology</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - pharmacology</topic><topic>Heparin - pharmacology</topic><topic>Immunohistochemistry - methods</topic><topic>Inositol 1,4,5-Trisphosphate Receptors</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Membrane Potentials - radiation effects</topic><topic>Microscopy, Confocal - methods</topic><topic>Organic Chemicals - metabolism</topic><topic>Patch-Clamp Techniques - methods</topic><topic>Phenylacetates - pharmacology</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Retina - cytology</topic><topic>Ryanodine - pharmacology</topic><topic>Ryanodine Receptor Calcium Release Channel - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Warrier, Ajithkumar</creatorcontrib><creatorcontrib>Borges, Salvador</creatorcontrib><creatorcontrib>Dalcino, David</creatorcontrib><creatorcontrib>Walters, Cameron</creatorcontrib><creatorcontrib>Wilson, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Warrier, Ajithkumar</au><au>Borges, Salvador</au><au>Dalcino, David</au><au>Walters, Cameron</au><au>Wilson, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>94</volume><issue>6</issue><spage>4196</spage><epage>4208</epage><pages>4196-4208</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract>Section of Neurobiology, Physiology and Behavior, Division of Biological Sciences, University of California, Davis, California
Submitted 10 June 2005;
accepted in final form 17 August 2005
The Ca 2+ that promotes transmitter release is generally thought to enter presynaptic terminals through voltage-gated Ca 2+ channels. Using electrophysiology and Ca 2+ imaging, we show that, in amacrine cell dendrites, at least some of the Ca 2+ that triggers transmitter release comes from endoplasmic reticulum Ca 2+ stores. We show that both inositol 1,4,5-trisphosphate receptors (IP 3 Rs) and ryanodine receptors (RyRs) are present in these dendrites and both participate in the elevation of cytoplasmic [Ca 2+ ] during the brief depolarization of a dendrite. Only the Ca 2+ released through IP 3 Rs, however, seems to promote the release of transmitter. Antagonists for the IP 3 R reduced transmitter release, whereas RyR blockers had no effect. Application of an agonist for metabotropic glutamate receptor, known to liberate Ca 2+ from internal stores, enhanced both spontaneous and evoked transmitter release.
Address for reprint requests and other correspondence: M. Wilson, Section of Neurobiology, Physiology and Behavior, Div. of Biological Sciences, UC Davis, Davis, CA 95616 (E-mail: mcwilson{at}ucdavis.edu )</abstract><cop>United States</cop><pub>Am Phys Soc</pub><pmid>16293593</pmid><doi>10.1152/jn.00604.2005</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of neurophysiology, 2005-12, Vol.94 (6), p.4196-4208 |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amacrine Cells - drug effects Amacrine Cells - metabolism Animals Boron Compounds - pharmacology Caffeine - pharmacology Calcium - metabolism Calcium Channels - metabolism Calcium-Binding Proteins - metabolism Central Nervous System Stimulants - pharmacology Chick Embryo Diagnostic Imaging - methods Dose-Response Relationship, Drug Drug Interactions Electric Stimulation - methods Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - metabolism Evoked Potentials - drug effects Evoked Potentials - physiology Evoked Potentials - radiation effects Excitatory Amino Acid Agonists - pharmacology gamma-Aminobutyric Acid - metabolism gamma-Aminobutyric Acid - pharmacology Glycine - analogs & derivatives Glycine - pharmacology Heparin - pharmacology Immunohistochemistry - methods Inositol 1,4,5-Trisphosphate Receptors Membrane Potentials - drug effects Membrane Potentials - physiology Membrane Potentials - radiation effects Microscopy, Confocal - methods Organic Chemicals - metabolism Patch-Clamp Techniques - methods Phenylacetates - pharmacology Receptors, Cytoplasmic and Nuclear - metabolism Retina - cytology Ryanodine - pharmacology Ryanodine Receptor Calcium Release Channel - metabolism Time Factors |
| title | Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells |
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