Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016
Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2‐year sentinel surveillance study. A total of 1639 rotaviru...
Gespeichert in:
| Veröffentlicht in: | Journal of medical virology 2018-02, Vol.90 (2), p.229-238 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 238 |
|---|---|
| container_issue | 2 |
| container_start_page | 229 |
| container_title | Journal of medical virology |
| container_volume | 90 |
| creator | Durmaz, Riza Bakkaloglu, Zekiye Unaldi, Ozlem Karagoz, Alper Korukluoglu, Gulay Kalaycioglu, Atila T. Acar, Sumeyra Kilic, Selcuk |
| description | Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2‐year sentinel surveillance study. A total of 1639 rotavirus antigen‐positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT‐PCR) with consensus primers for the VP7 and VP4 genes, followed by semi‐nested type‐specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0‐12 months age group, followed by children in the 13‐24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014‐2016. |
| doi_str_mv | 10.1002/jmv.24945 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1937759113</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1973568244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-8a3a26bb5376cbc86d9a41744a7cc9b09393187e8c579341bfdb3d52dfe8b6a63</originalsourceid><addsrcrecordid>eNp10c9KHTEUBvBQWuqt7cIXkEA3Co7mfyZLEW0tSruw3Q6Z5Izkdu7MbTIzMjvpE_QZ-yTmerULoasTDj--HPgQ2qPkmBLCTpar6ZgJI-QrtKDEqMIQTV-jBaFCFUpRuYPepbQkhJSGsbdoh5X5QTVfoN_fIky2hc4Btp3HPkwQUxhm3Dc49oOdQhwTPsW30PXDvIaEXd60dgjdLQ4dvhnjT5ixH-NmYTH7e_9nBhtxgi4baHEa4wShbe3mjzXE0PsjzPJtWeah3qM3jW0TfHiau-j7xfnN2efi6uuny7PTq8JxyWVRWm6ZqmvJtXK1K5U3VlAthNXOmZoYbjgtNZROasMFrRtfcy-Zb6CslVV8Fx1sc9ex_zVCGqpVSA42h0E_pooarrU0lPJMP76gy36MXb4uK82lKpkQWR1ulYt9ShGaah3Dysa5oqTaFFPlYqrHYrLdf0oc6xX4f_K5iQxOtuAutDD_P6n6cv1jG_kAwbWZUw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1973568244</pqid></control><display><type>article</type><title>Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Durmaz, Riza ; Bakkaloglu, Zekiye ; Unaldi, Ozlem ; Karagoz, Alper ; Korukluoglu, Gulay ; Kalaycioglu, Atila T. ; Acar, Sumeyra ; Kilic, Selcuk</creator><creatorcontrib>Durmaz, Riza ; Bakkaloglu, Zekiye ; Unaldi, Ozlem ; Karagoz, Alper ; Korukluoglu, Gulay ; Kalaycioglu, Atila T. ; Acar, Sumeyra ; Kilic, Selcuk ; Turkish Rotavirus Surveillance Network ; The Turkish Rotavirus Surveillance Network</creatorcontrib><description>Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2‐year sentinel surveillance study. A total of 1639 rotavirus antigen‐positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT‐PCR) with consensus primers for the VP7 and VP4 genes, followed by semi‐nested type‐specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0‐12 months age group, followed by children in the 13‐24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014‐2016.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.24945</identifier><identifier>PMID: 28892173</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Age ; Child, Preschool ; Children ; Diarrhea ; Etiology ; Feces - virology ; Female ; Gastroenteritis ; Genetic Variation ; Genotype ; Genotypes ; Genotyping Techniques ; Humans ; Infant ; Infant, Newborn ; Male ; Molecular Epidemiology ; Multiplexing ; Polymerase chain reaction ; Prevalence ; Primers ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse transcription ; Ribonucleic acid ; RNA ; RNA, Viral - genetics ; Rotavirus ; Rotavirus - classification ; Rotavirus - genetics ; Rotavirus - isolation & purification ; Rotavirus Infections - epidemiology ; Rotavirus Infections - virology ; Sentinel Surveillance ; Surveillance ; Turkey - epidemiology ; Virology ; Viruses</subject><ispartof>Journal of medical virology, 2018-02, Vol.90 (2), p.229-238</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-8a3a26bb5376cbc86d9a41744a7cc9b09393187e8c579341bfdb3d52dfe8b6a63</citedby><cites>FETCH-LOGICAL-c3535-8a3a26bb5376cbc86d9a41744a7cc9b09393187e8c579341bfdb3d52dfe8b6a63</cites><orcidid>0000-0003-3247-3577</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.24945$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.24945$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28892173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durmaz, Riza</creatorcontrib><creatorcontrib>Bakkaloglu, Zekiye</creatorcontrib><creatorcontrib>Unaldi, Ozlem</creatorcontrib><creatorcontrib>Karagoz, Alper</creatorcontrib><creatorcontrib>Korukluoglu, Gulay</creatorcontrib><creatorcontrib>Kalaycioglu, Atila T.</creatorcontrib><creatorcontrib>Acar, Sumeyra</creatorcontrib><creatorcontrib>Kilic, Selcuk</creatorcontrib><creatorcontrib>Turkish Rotavirus Surveillance Network</creatorcontrib><creatorcontrib>The Turkish Rotavirus Surveillance Network</creatorcontrib><title>Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2‐year sentinel surveillance study. A total of 1639 rotavirus antigen‐positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT‐PCR) with consensus primers for the VP7 and VP4 genes, followed by semi‐nested type‐specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0‐12 months age group, followed by children in the 13‐24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014‐2016.</description><subject>Age</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Diarrhea</subject><subject>Etiology</subject><subject>Feces - virology</subject><subject>Female</subject><subject>Gastroenteritis</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Genotyping Techniques</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Molecular Epidemiology</subject><subject>Multiplexing</subject><subject>Polymerase chain reaction</subject><subject>Prevalence</subject><subject>Primers</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Reverse transcription</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>Rotavirus</subject><subject>Rotavirus - classification</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - isolation & purification</subject><subject>Rotavirus Infections - epidemiology</subject><subject>Rotavirus Infections - virology</subject><subject>Sentinel Surveillance</subject><subject>Surveillance</subject><subject>Turkey - epidemiology</subject><subject>Virology</subject><subject>Viruses</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c9KHTEUBvBQWuqt7cIXkEA3Co7mfyZLEW0tSruw3Q6Z5Izkdu7MbTIzMjvpE_QZ-yTmerULoasTDj--HPgQ2qPkmBLCTpar6ZgJI-QrtKDEqMIQTV-jBaFCFUpRuYPepbQkhJSGsbdoh5X5QTVfoN_fIky2hc4Btp3HPkwQUxhm3Dc49oOdQhwTPsW30PXDvIaEXd60dgjdLQ4dvhnjT5ixH-NmYTH7e_9nBhtxgi4baHEa4wShbe3mjzXE0PsjzPJtWeah3qM3jW0TfHiau-j7xfnN2efi6uuny7PTq8JxyWVRWm6ZqmvJtXK1K5U3VlAthNXOmZoYbjgtNZROasMFrRtfcy-Zb6CslVV8Fx1sc9ex_zVCGqpVSA42h0E_pooarrU0lPJMP76gy36MXb4uK82lKpkQWR1ulYt9ShGaah3Dysa5oqTaFFPlYqrHYrLdf0oc6xX4f_K5iQxOtuAutDD_P6n6cv1jG_kAwbWZUw</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Durmaz, Riza</creator><creator>Bakkaloglu, Zekiye</creator><creator>Unaldi, Ozlem</creator><creator>Karagoz, Alper</creator><creator>Korukluoglu, Gulay</creator><creator>Kalaycioglu, Atila T.</creator><creator>Acar, Sumeyra</creator><creator>Kilic, Selcuk</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3247-3577</orcidid></search><sort><creationdate>201802</creationdate><title>Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016</title><author>Durmaz, Riza ; Bakkaloglu, Zekiye ; Unaldi, Ozlem ; Karagoz, Alper ; Korukluoglu, Gulay ; Kalaycioglu, Atila T. ; Acar, Sumeyra ; Kilic, Selcuk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-8a3a26bb5376cbc86d9a41744a7cc9b09393187e8c579341bfdb3d52dfe8b6a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Age</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Diarrhea</topic><topic>Etiology</topic><topic>Feces - virology</topic><topic>Female</topic><topic>Gastroenteritis</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Genotyping Techniques</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Molecular Epidemiology</topic><topic>Multiplexing</topic><topic>Polymerase chain reaction</topic><topic>Prevalence</topic><topic>Primers</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Reverse transcription</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>Rotavirus</topic><topic>Rotavirus - classification</topic><topic>Rotavirus - genetics</topic><topic>Rotavirus - isolation & purification</topic><topic>Rotavirus Infections - epidemiology</topic><topic>Rotavirus Infections - virology</topic><topic>Sentinel Surveillance</topic><topic>Surveillance</topic><topic>Turkey - epidemiology</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durmaz, Riza</creatorcontrib><creatorcontrib>Bakkaloglu, Zekiye</creatorcontrib><creatorcontrib>Unaldi, Ozlem</creatorcontrib><creatorcontrib>Karagoz, Alper</creatorcontrib><creatorcontrib>Korukluoglu, Gulay</creatorcontrib><creatorcontrib>Kalaycioglu, Atila T.</creatorcontrib><creatorcontrib>Acar, Sumeyra</creatorcontrib><creatorcontrib>Kilic, Selcuk</creatorcontrib><creatorcontrib>Turkish Rotavirus Surveillance Network</creatorcontrib><creatorcontrib>The Turkish Rotavirus Surveillance Network</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durmaz, Riza</au><au>Bakkaloglu, Zekiye</au><au>Unaldi, Ozlem</au><au>Karagoz, Alper</au><au>Korukluoglu, Gulay</au><au>Kalaycioglu, Atila T.</au><au>Acar, Sumeyra</au><au>Kilic, Selcuk</au><aucorp>Turkish Rotavirus Surveillance Network</aucorp><aucorp>The Turkish Rotavirus Surveillance Network</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J Med Virol</addtitle><date>2018-02</date><risdate>2018</risdate><volume>90</volume><issue>2</issue><spage>229</spage><epage>238</epage><pages>229-238</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><abstract>Human rotavirus A (RVA) is the main etiological agent of watery diarrhea among children under 5 years of age worldwide. The aims of this study were to investigate the prevalence and diversity of RVA genotypes circulating in Turkey during a 2‐year sentinel surveillance study. A total of 1639 rotavirus antigen‐positive stool samples were obtained from children younger than 5 years of age hospitalized with acute gastroenteritis. Rotavirus G and P genotypes were determined by reverse transcription polymerase chain reaction (RT‐PCR) with consensus primers for the VP7 and VP4 genes, followed by semi‐nested type‐specific multiplex PCR. Rotavirus RNA was detected in 1396 (85.3%) of the samples tested. The highest detection rate (38.2%) was obtained among children in the 0‐12 months age group, followed by children in the 13‐24 months age group (36.2%). The most prevalent genotype was G1P[8] (24.6%) followed by G3P[8] (19.6%), G9P[8] (12.2%), G2P[4] (9.5%), G2P[8] (6.5%), and G4P[8] (4.8%). The proportions of uncommon and mixed genotypes were 21.5% and 1.14%, respectively. The large number of genotypes observed, including common, uncommon, and mixed types, indicates a high heterogeneity of RVA strains circulating in Turkey. The current study also exhibited dramatic fluctuations on the prevalences of the common genotypes, with increases in G3 and G1 and decreases in G9 and G2 from 2014‐2016.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28892173</pmid><doi>10.1002/jmv.24945</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3247-3577</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 2018-02, Vol.90 (2), p.229-238 |
| issn | 0146-6615 1096-9071 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1937759113 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Age Child, Preschool Children Diarrhea Etiology Feces - virology Female Gastroenteritis Genetic Variation Genotype Genotypes Genotyping Techniques Humans Infant Infant, Newborn Male Molecular Epidemiology Multiplexing Polymerase chain reaction Prevalence Primers Reverse Transcriptase Polymerase Chain Reaction Reverse transcription Ribonucleic acid RNA RNA, Viral - genetics Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotavirus Infections - epidemiology Rotavirus Infections - virology Sentinel Surveillance Surveillance Turkey - epidemiology Virology Viruses |
| title | Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A02%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20and%20diversity%20of%20rotavirus%20A%20genotypes%20cirulating%20in%20Turkey%20during%20a%202%E2%80%90year%20sentinel%20surveillance%20period,%202014%E2%80%902016&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Durmaz,%20Riza&rft.aucorp=Turkish%20Rotavirus%20Surveillance%20Network&rft.date=2018-02&rft.volume=90&rft.issue=2&rft.spage=229&rft.epage=238&rft.pages=229-238&rft.issn=0146-6615&rft.eissn=1096-9071&rft_id=info:doi/10.1002/jmv.24945&rft_dat=%3Cproquest_cross%3E1973568244%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1973568244&rft_id=info:pmid/28892173&rfr_iscdi=true |