Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats
Purpose Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of the reproductive system characterized by polycystic ovaries and androgen excess. Letrozole is a nonsteroidal aromatase inhibitor that is used in experimental research to induce PCOS. Kisspeptin is an essential protein...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2017-11, Vol.296 (5), p.957-963 |
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| creator | Aliabadi, Elham Namavar, Mohammad Reza Mortezaee, Keywan Toolee, Heidar Keshtgar, Sara Mirkhani, Hossein Akbari, Mohammad Rastegar, Tayebeh Solhjoo, Somayeh |
| description | Purpose
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of the reproductive system characterized by polycystic ovaries and androgen excess. Letrozole is a nonsteroidal aromatase inhibitor that is used in experimental research to induce PCOS. Kisspeptin is an essential protein in regulation of cyclicity. Kisspeptin receptor is expressed in the hypothalamus and pituitary glands, and kisspeptin containing neurons are affected from sex steroid hormones. We aimed to investigate the number of kisspeptin-positive cells in the arcuate (Arc) and anteroventral periventricular nuclei (AVPV) of hypothalamus in the letrozole-induced PCOS.
Methods
40 female Wistar rats were divided into the proestrus control, diestrus control, proestrus vehicle, diestrus vehicle and letrozole. Animals were sacrificed after 3 weeks, and sera, ovary and brain samples were harvested for further evaluations.
Results
Letrozole group had high weight gain, high numbers of ovarian follicular cysts, high levels of luteinizing hormone and testosterone and increase number of kisspeptin-positive cells in the Arc nucleus, as compared with the control groups (
P
≤ 0.05 vs. proestrus control and proestrus vehicle). Letrozole group showed a decrease in the number of kisspeptin-positive cells in the AVPV nucleus (
P
≤ 0.05 vs. proestrus control and proestrus vehicle).
Conclusion
Our findings show that the number of kisspeptin-positive cells may be affected from letrozole, and that the changes in the number of these cells may be in favor of the appearance of PCOS features in this group. |
| doi_str_mv | 10.1007/s00404-017-4509-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1936268701</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2261529349</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-5c0af400ea41d8af57f02804c26320b37f1f5b38b01e1253b1084e716b6054433</originalsourceid><addsrcrecordid>eNp1kcuKFTEQhoMoznH0AdxIwI2b1sqlb0sZRkcccKPrJp2u9mRIJ20uw7Qv46ua9owKgovwVyVf_ir4CXnO4DUDaN9EAAmyAtZWsoa-Eg_IgUnBK2gZe0gO0O81NO0ZeRLjDQDjXdc8JmdF2lqw_kB-fDQxrrgm4yjerQFjNN7RGVXKpaHlOh2RqqCzSkXdVE7C4G_RpaAsXTGYX7XR2apAXdYWDfUzPW6rT0dl1ZLj3ltMwX_3Fivjpqxxoqu3m95iMpr6WxWMcjRubgp-wX1wUCk-JY9mZSM-u9dz8uXd5eeLq-r60_sPF2-vKy1Fl6pag5olACrJpk7NdTsD70Bq3ggOo2hnNtej6EZgyHgtRgadxJY1YwO1lEKck1cn3zX4bxljGhYTNVqrHPocB9aLhjddC6ygL_9Bb3wOrmw3cN6wmvdC9oViJ0oHH2PAeViDWVTYBgbDnt5wSm8o6Q17esO-xIt75zwuOP358TuuAvATEMuT-4rh7-j_u_4E1IyoZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2261529349</pqid></control><display><type>article</type><title>Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Aliabadi, Elham ; Namavar, Mohammad Reza ; Mortezaee, Keywan ; Toolee, Heidar ; Keshtgar, Sara ; Mirkhani, Hossein ; Akbari, Mohammad ; Rastegar, Tayebeh ; Solhjoo, Somayeh</creator><creatorcontrib>Aliabadi, Elham ; Namavar, Mohammad Reza ; Mortezaee, Keywan ; Toolee, Heidar ; Keshtgar, Sara ; Mirkhani, Hossein ; Akbari, Mohammad ; Rastegar, Tayebeh ; Solhjoo, Somayeh</creatorcontrib><description>Purpose
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of the reproductive system characterized by polycystic ovaries and androgen excess. Letrozole is a nonsteroidal aromatase inhibitor that is used in experimental research to induce PCOS. Kisspeptin is an essential protein in regulation of cyclicity. Kisspeptin receptor is expressed in the hypothalamus and pituitary glands, and kisspeptin containing neurons are affected from sex steroid hormones. We aimed to investigate the number of kisspeptin-positive cells in the arcuate (Arc) and anteroventral periventricular nuclei (AVPV) of hypothalamus in the letrozole-induced PCOS.
Methods
40 female Wistar rats were divided into the proestrus control, diestrus control, proestrus vehicle, diestrus vehicle and letrozole. Animals were sacrificed after 3 weeks, and sera, ovary and brain samples were harvested for further evaluations.
Results
Letrozole group had high weight gain, high numbers of ovarian follicular cysts, high levels of luteinizing hormone and testosterone and increase number of kisspeptin-positive cells in the Arc nucleus, as compared with the control groups (
P
≤ 0.05 vs. proestrus control and proestrus vehicle). Letrozole group showed a decrease in the number of kisspeptin-positive cells in the AVPV nucleus (
P
≤ 0.05 vs. proestrus control and proestrus vehicle).
Conclusion
Our findings show that the number of kisspeptin-positive cells may be affected from letrozole, and that the changes in the number of these cells may be in favor of the appearance of PCOS features in this group.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-017-4509-3</identifier><identifier>PMID: 28875319</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Arcuate Nucleus of Hypothalamus - metabolism ; Endocrinology ; Female ; General Gynecology ; Gonadal Steroid Hormones - blood ; Gynecology ; Human Genetics ; Humans ; Hypothalamus ; Hypothalamus, Anterior - metabolism ; Kisspeptins - metabolism ; Luteinizing Hormone - blood ; Medicine ; Medicine & Public Health ; Neurons ; Nitriles - pharmacology ; Obstetrics/Perinatology/Midwifery ; Pituitary Gland ; Polycystic ovary syndrome ; Polycystic Ovary Syndrome - chemically induced ; Rats ; Rats, Wistar ; Rodents ; Testosterone - blood ; Triazoles - pharmacology</subject><ispartof>Archives of gynecology and obstetrics, 2017-11, Vol.296 (5), p.957-963</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Archives of Gynecology and Obstetrics is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-5c0af400ea41d8af57f02804c26320b37f1f5b38b01e1253b1084e716b6054433</citedby><cites>FETCH-LOGICAL-c438t-5c0af400ea41d8af57f02804c26320b37f1f5b38b01e1253b1084e716b6054433</cites><orcidid>0000-0001-6540-9470 ; 0000-0003-2004-3465</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-017-4509-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-017-4509-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28875319$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aliabadi, Elham</creatorcontrib><creatorcontrib>Namavar, Mohammad Reza</creatorcontrib><creatorcontrib>Mortezaee, Keywan</creatorcontrib><creatorcontrib>Toolee, Heidar</creatorcontrib><creatorcontrib>Keshtgar, Sara</creatorcontrib><creatorcontrib>Mirkhani, Hossein</creatorcontrib><creatorcontrib>Akbari, Mohammad</creatorcontrib><creatorcontrib>Rastegar, Tayebeh</creatorcontrib><creatorcontrib>Solhjoo, Somayeh</creatorcontrib><title>Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of the reproductive system characterized by polycystic ovaries and androgen excess. Letrozole is a nonsteroidal aromatase inhibitor that is used in experimental research to induce PCOS. Kisspeptin is an essential protein in regulation of cyclicity. Kisspeptin receptor is expressed in the hypothalamus and pituitary glands, and kisspeptin containing neurons are affected from sex steroid hormones. We aimed to investigate the number of kisspeptin-positive cells in the arcuate (Arc) and anteroventral periventricular nuclei (AVPV) of hypothalamus in the letrozole-induced PCOS.
Methods
40 female Wistar rats were divided into the proestrus control, diestrus control, proestrus vehicle, diestrus vehicle and letrozole. Animals were sacrificed after 3 weeks, and sera, ovary and brain samples were harvested for further evaluations.
Results
Letrozole group had high weight gain, high numbers of ovarian follicular cysts, high levels of luteinizing hormone and testosterone and increase number of kisspeptin-positive cells in the Arc nucleus, as compared with the control groups (
P
≤ 0.05 vs. proestrus control and proestrus vehicle). Letrozole group showed a decrease in the number of kisspeptin-positive cells in the AVPV nucleus (
P
≤ 0.05 vs. proestrus control and proestrus vehicle).
Conclusion
Our findings show that the number of kisspeptin-positive cells may be affected from letrozole, and that the changes in the number of these cells may be in favor of the appearance of PCOS features in this group.</description><subject>Animals</subject><subject>Arcuate Nucleus of Hypothalamus - metabolism</subject><subject>Endocrinology</subject><subject>Female</subject><subject>General Gynecology</subject><subject>Gonadal Steroid Hormones - blood</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Hypothalamus</subject><subject>Hypothalamus, Anterior - metabolism</subject><subject>Kisspeptins - metabolism</subject><subject>Luteinizing Hormone - blood</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neurons</subject><subject>Nitriles - pharmacology</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Pituitary Gland</subject><subject>Polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - chemically induced</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Testosterone - blood</subject><subject>Triazoles - pharmacology</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kcuKFTEQhoMoznH0AdxIwI2b1sqlb0sZRkcccKPrJp2u9mRIJ20uw7Qv46ua9owKgovwVyVf_ir4CXnO4DUDaN9EAAmyAtZWsoa-Eg_IgUnBK2gZe0gO0O81NO0ZeRLjDQDjXdc8JmdF2lqw_kB-fDQxrrgm4yjerQFjNN7RGVXKpaHlOh2RqqCzSkXdVE7C4G_RpaAsXTGYX7XR2apAXdYWDfUzPW6rT0dl1ZLj3ltMwX_3Fivjpqxxoqu3m95iMpr6WxWMcjRubgp-wX1wUCk-JY9mZSM-u9dz8uXd5eeLq-r60_sPF2-vKy1Fl6pag5olACrJpk7NdTsD70Bq3ggOo2hnNtej6EZgyHgtRgadxJY1YwO1lEKck1cn3zX4bxljGhYTNVqrHPocB9aLhjddC6ygL_9Bb3wOrmw3cN6wmvdC9oViJ0oHH2PAeViDWVTYBgbDnt5wSm8o6Q17esO-xIt75zwuOP358TuuAvATEMuT-4rh7-j_u_4E1IyoZw</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Aliabadi, Elham</creator><creator>Namavar, Mohammad Reza</creator><creator>Mortezaee, Keywan</creator><creator>Toolee, Heidar</creator><creator>Keshtgar, Sara</creator><creator>Mirkhani, Hossein</creator><creator>Akbari, Mohammad</creator><creator>Rastegar, Tayebeh</creator><creator>Solhjoo, Somayeh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6540-9470</orcidid><orcidid>https://orcid.org/0000-0003-2004-3465</orcidid></search><sort><creationdate>20171101</creationdate><title>Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats</title><author>Aliabadi, Elham ; Namavar, Mohammad Reza ; Mortezaee, Keywan ; Toolee, Heidar ; Keshtgar, Sara ; Mirkhani, Hossein ; Akbari, Mohammad ; Rastegar, Tayebeh ; Solhjoo, Somayeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-5c0af400ea41d8af57f02804c26320b37f1f5b38b01e1253b1084e716b6054433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Arcuate Nucleus of Hypothalamus - metabolism</topic><topic>Endocrinology</topic><topic>Female</topic><topic>General Gynecology</topic><topic>Gonadal Steroid Hormones - blood</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Hypothalamus</topic><topic>Hypothalamus, Anterior - metabolism</topic><topic>Kisspeptins - metabolism</topic><topic>Luteinizing Hormone - blood</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neurons</topic><topic>Nitriles - pharmacology</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Pituitary Gland</topic><topic>Polycystic ovary syndrome</topic><topic>Polycystic Ovary Syndrome - chemically induced</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Testosterone - blood</topic><topic>Triazoles - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aliabadi, Elham</creatorcontrib><creatorcontrib>Namavar, Mohammad Reza</creatorcontrib><creatorcontrib>Mortezaee, Keywan</creatorcontrib><creatorcontrib>Toolee, Heidar</creatorcontrib><creatorcontrib>Keshtgar, Sara</creatorcontrib><creatorcontrib>Mirkhani, Hossein</creatorcontrib><creatorcontrib>Akbari, Mohammad</creatorcontrib><creatorcontrib>Rastegar, Tayebeh</creatorcontrib><creatorcontrib>Solhjoo, Somayeh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aliabadi, Elham</au><au>Namavar, Mohammad Reza</au><au>Mortezaee, Keywan</au><au>Toolee, Heidar</au><au>Keshtgar, Sara</au><au>Mirkhani, Hossein</au><au>Akbari, Mohammad</au><au>Rastegar, Tayebeh</au><au>Solhjoo, Somayeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>296</volume><issue>5</issue><spage>957</spage><epage>963</epage><pages>957-963</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Purpose
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of the reproductive system characterized by polycystic ovaries and androgen excess. Letrozole is a nonsteroidal aromatase inhibitor that is used in experimental research to induce PCOS. Kisspeptin is an essential protein in regulation of cyclicity. Kisspeptin receptor is expressed in the hypothalamus and pituitary glands, and kisspeptin containing neurons are affected from sex steroid hormones. We aimed to investigate the number of kisspeptin-positive cells in the arcuate (Arc) and anteroventral periventricular nuclei (AVPV) of hypothalamus in the letrozole-induced PCOS.
Methods
40 female Wistar rats were divided into the proestrus control, diestrus control, proestrus vehicle, diestrus vehicle and letrozole. Animals were sacrificed after 3 weeks, and sera, ovary and brain samples were harvested for further evaluations.
Results
Letrozole group had high weight gain, high numbers of ovarian follicular cysts, high levels of luteinizing hormone and testosterone and increase number of kisspeptin-positive cells in the Arc nucleus, as compared with the control groups (
P
≤ 0.05 vs. proestrus control and proestrus vehicle). Letrozole group showed a decrease in the number of kisspeptin-positive cells in the AVPV nucleus (
P
≤ 0.05 vs. proestrus control and proestrus vehicle).
Conclusion
Our findings show that the number of kisspeptin-positive cells may be affected from letrozole, and that the changes in the number of these cells may be in favor of the appearance of PCOS features in this group.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28875319</pmid><doi>10.1007/s00404-017-4509-3</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-6540-9470</orcidid><orcidid>https://orcid.org/0000-0003-2004-3465</orcidid></addata></record> |
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| ispartof | Archives of gynecology and obstetrics, 2017-11, Vol.296 (5), p.957-963 |
| issn | 0932-0067 1432-0711 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Arcuate Nucleus of Hypothalamus - metabolism Endocrinology Female General Gynecology Gonadal Steroid Hormones - blood Gynecology Human Genetics Humans Hypothalamus Hypothalamus, Anterior - metabolism Kisspeptins - metabolism Luteinizing Hormone - blood Medicine Medicine & Public Health Neurons Nitriles - pharmacology Obstetrics/Perinatology/Midwifery Pituitary Gland Polycystic ovary syndrome Polycystic Ovary Syndrome - chemically induced Rats Rats, Wistar Rodents Testosterone - blood Triazoles - pharmacology |
| title | Kisspeptin expression features in the arcuate and anteroventral periventricular nuclei of hypothalamus of letrozole-induced polycystic ovarian syndrome in rats |
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