Mass spectrometry based identification of galectin-3 interacting proteins potentially involved in lung melanoma metastasis
Adhesive interactions between molecules on tumor cells and those on target organs play a key role in organ specific metastasis. Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating...
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| Veröffentlicht in: | Molecular bioSystems 2017, Vol.13 (11), p.2303-2309 |
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| container_title | Molecular bioSystems |
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| creator | Dange, Manohar C Bhonsle, Hemangi S Godbole, Rashmi K More, Shyam K Bane, Sanjay M Kulkarni, Mahesh J Kalraiya, Rajiv D |
| description | Adhesive interactions between molecules on tumor cells and those on target organs play a key role in organ specific metastasis. Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating their arrest and extravasation. This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. A total of 83 proteins were identified as galectin-3 interacting glycoproteins, of which 35 were constituents of the L-PHA bound fraction, suggesting that these proteins carry polyLacNAc substituted β1,6 branched N-glycans. The identities of some of these proteins, like LAMP-1, LAMP-3, basigin, embigin, and α5 and β1 Integrin, have been confirmed by western blotting, and functional relevance with respect to metastatic properties has been established. |
| doi_str_mv | 10.1039/c7mb00260b |
| format | Article |
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Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating their arrest and extravasation. This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. A total of 83 proteins were identified as galectin-3 interacting glycoproteins, of which 35 were constituents of the L-PHA bound fraction, suggesting that these proteins carry polyLacNAc substituted β1,6 branched N-glycans. 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Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating their arrest and extravasation. This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. A total of 83 proteins were identified as galectin-3 interacting glycoproteins, of which 35 were constituents of the L-PHA bound fraction, suggesting that these proteins carry polyLacNAc substituted β1,6 branched N-glycans. 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Poly-N-acetyl-lactosamine (polyLacNAc) substituted N-oligosaccharides on melanoma cell surface glycoproteins promote lung specific metastasis via galectin-3 by facilitating their arrest and extravasation. This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. A total of 83 proteins were identified as galectin-3 interacting glycoproteins, of which 35 were constituents of the L-PHA bound fraction, suggesting that these proteins carry polyLacNAc substituted β1,6 branched N-glycans. 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| subjects | Animals Carrier Proteins - metabolism CD147 antigen Cell adhesion Cell surface Chromatography, Affinity Chromatography, Liquid Extravasation Galectin 3 - metabolism Galectin-3 Glycoproteins LAMP-1 protein Lung Neoplasms - metabolism Lung Neoplasms - secondary Lungs Mass spectrometry Mass Spectrometry - methods Mass spectroscopy Melanoma Melanoma - pathology Melanoma, Experimental Metastases Metastasis Mice N-glycans Oligosaccharides Organs Polysaccharides Protein Binding Protein Interaction Mapping - methods Proteins Reproducibility of Results Substitutes Tumor cells Western blotting Workflow |
| title | Mass spectrometry based identification of galectin-3 interacting proteins potentially involved in lung melanoma metastasis |
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