Anti-inflammatory effects of dulse (Palmaria palmata) resulting from the simultaneous water-extraction of phycobiliproteins and chlorophyll a

The use of dulse (Palmaria palmata) as a source of edible anti-inflammatory products was evaluated in this study. Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digest...

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Veröffentlicht in:Food research international 2017-10, Vol.100 (Pt 1), p.514-521
Hauptverfasser: Lee, Daeyoung, Nishizawa, Mizuho, Shimizu, Yutaka, Saeki, Hiroki
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creator Lee, Daeyoung
Nishizawa, Mizuho
Shimizu, Yutaka
Saeki, Hiroki
description The use of dulse (Palmaria palmata) as a source of edible anti-inflammatory products was evaluated in this study. Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-α, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin β-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse. [Display omitted] •Water extraction of extracted from lyophilized dulse leaves contained phycobiliproteins and chlorophyll a.•The thermolysin-digested water extract showed anti-inflammatory activity in vitro and in vivo.•Decapeptide derived from phycoerythrin β-chain and chlorophyll a decomposition products showed anti-inflammatory activity.•The anti-inflammatory activity would be caused by phycobiliprotein and chlorophyll a decomposition products.
doi_str_mv 10.1016/j.foodres.2017.06.040
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Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-α, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin β-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse. [Display omitted] •Water extraction of extracted from lyophilized dulse leaves contained phycobiliproteins and chlorophyll a.•The thermolysin-digested water extract showed anti-inflammatory activity in vitro and in vivo.•Decapeptide derived from phycoerythrin β-chain and chlorophyll a decomposition products showed anti-inflammatory activity.•The anti-inflammatory activity would be caused by phycobiliprotein and chlorophyll a decomposition products.</description><identifier>ISSN: 0963-9969</identifier><identifier>EISSN: 1873-7145</identifier><identifier>DOI: 10.1016/j.foodres.2017.06.040</identifier><identifier>PMID: 28873715</identifier><language>eng</language><publisher>Canada: Elsevier Ltd</publisher><subject>Animals ; Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - isolation &amp; purification ; Anti-Inflammatory Agents - pharmacology ; Anti-inflammatory effect ; Chlorophyll ; Chlorophyll A - chemistry ; Chlorophyll A - isolation &amp; purification ; Chlorophyll A - pharmacology ; Cytokines - analysis ; Cytokines - metabolism ; Dulse ; Liquid-Liquid Extraction ; Macrophages - drug effects ; Macrophages - metabolism ; Mice ; Pheophorbide ; Phycobiliprotein ; Phycobiliproteins - chemistry ; Phycobiliproteins - isolation &amp; purification ; Phycobiliproteins - pharmacology ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; RAW 264.7 Cells ; Rhodophyta - chemistry ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Water-extract</subject><ispartof>Food research international, 2017-10, Vol.100 (Pt 1), p.514-521</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. 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Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-α, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin β-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse. 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purification</subject><subject>Phycobiliproteins - pharmacology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>RAW 264.7 Cells</subject><subject>Rhodophyta - chemistry</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Water-extract</subject><issn>0963-9969</issn><issn>1873-7145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRCILoWfAPKxPSQdJ46TnFBVlQ-pEhzgbDn2mPXKiRfboeyP6H_Gq124cvLI8-a9mfcIecugZsDEza62IZiIqW6A9TWIGjg8Ixs29G3VM949JxsYRVuNoxgvyKuUdgAgun58SS6aoaB61m3I0-2SXeUW69U8qxzigaK1qHOiwVKz-oT06qvys4pO0f2xyOqaFt3VZ7f8oDaGmeYt0uTm8qUWDGuijypjrPB3jkpnF5Yj2X570GFy3u1jyOiWRNViqN76EEPpeU_Va_LCqiL55vxeku8f7r_dfaoevnz8fHf7UGneslwJAwM2jDFsECZuoRu6yXLDu6ERYCzjw8R5NzUTb5ADQ8utAq0UigbazrSX5OrEW1b5uWLKcnZJo_en9SUbW1GYWCMKtDtBdQwpRbRyH11x4yAZyGMScifPSchjEhKELEmUuXdniXWa0fyb-mt9Abw_AbAc-sthlEk7XDQaF4v_0gT3H4k_edGggw</recordid><startdate>201710</startdate><enddate>201710</enddate><creator>Lee, Daeyoung</creator><creator>Nishizawa, Mizuho</creator><creator>Shimizu, Yutaka</creator><creator>Saeki, Hiroki</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201710</creationdate><title>Anti-inflammatory effects of dulse (Palmaria palmata) resulting from the simultaneous water-extraction of phycobiliproteins and chlorophyll a</title><author>Lee, Daeyoung ; Nishizawa, Mizuho ; Shimizu, Yutaka ; Saeki, Hiroki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-6d08e2111e2e0b4f0585bf4d458260df148b445b2b42e401ef4fa0caae62035d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - chemistry</topic><topic>Anti-Inflammatory Agents - isolation &amp; purification</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Anti-inflammatory effect</topic><topic>Chlorophyll</topic><topic>Chlorophyll A - chemistry</topic><topic>Chlorophyll A - isolation &amp; purification</topic><topic>Chlorophyll A - pharmacology</topic><topic>Cytokines - analysis</topic><topic>Cytokines - metabolism</topic><topic>Dulse</topic><topic>Liquid-Liquid Extraction</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>Pheophorbide</topic><topic>Phycobiliprotein</topic><topic>Phycobiliproteins - chemistry</topic><topic>Phycobiliproteins - isolation &amp; purification</topic><topic>Phycobiliproteins - pharmacology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>RAW 264.7 Cells</topic><topic>Rhodophyta - chemistry</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Water-extract</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Daeyoung</creatorcontrib><creatorcontrib>Nishizawa, Mizuho</creatorcontrib><creatorcontrib>Shimizu, Yutaka</creatorcontrib><creatorcontrib>Saeki, Hiroki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Daeyoung</au><au>Nishizawa, Mizuho</au><au>Shimizu, Yutaka</au><au>Saeki, Hiroki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory effects of dulse (Palmaria palmata) resulting from the simultaneous water-extraction of phycobiliproteins and chlorophyll a</atitle><jtitle>Food research international</jtitle><addtitle>Food Res Int</addtitle><date>2017-10</date><risdate>2017</risdate><volume>100</volume><issue>Pt 1</issue><spage>514</spage><epage>521</epage><pages>514-521</pages><issn>0963-9969</issn><eissn>1873-7145</eissn><abstract>The use of dulse (Palmaria palmata) as a source of edible anti-inflammatory products was evaluated in this study. Phycobiliproteins and chlorophyll a were simultaneously extracted from lyophilized dulse leaves via water-extraction, and subjected to thermolysin digestion to produce thermolysin-digested water-extract (d-DWE). d-DWE significantly reduced tumor necrosis factor-α, interleukin-6, and nitric oxide in LPS-stimulated murine macrophages (RAW 264.7 cells), and orally administered d-DWE mitigated acute inflammation in carrageenan-induced paw edema of mice. Mass spectrometry revealed d-DWE contained peptide LRDGEIILRY (derived from phycoerythrin β-chain) and chlorophyll a decomposition products, and they individually reduced the secretion of the proinflammatory mediators in LPS-stimulated RAW 264.7 cells. These results indicate the anti-inflammatory activity could be from a combined effect of phycobiliprotein and chlorophyll a decomposition products prepared from the water-extract of dulse. Thus, inexpensive and safe water-extraction method is effective for the extraction of anti-inflammatory components from dulse. [Display omitted] •Water extraction of extracted from lyophilized dulse leaves contained phycobiliproteins and chlorophyll a.•The thermolysin-digested water extract showed anti-inflammatory activity in vitro and in vivo.•Decapeptide derived from phycoerythrin β-chain and chlorophyll a decomposition products showed anti-inflammatory activity.•The anti-inflammatory activity would be caused by phycobiliprotein and chlorophyll a decomposition products.</abstract><cop>Canada</cop><pub>Elsevier Ltd</pub><pmid>28873715</pmid><doi>10.1016/j.foodres.2017.06.040</doi><tpages>8</tpages></addata></record>
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Animals
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - isolation & purification
Anti-Inflammatory Agents - pharmacology
Anti-inflammatory effect
Chlorophyll
Chlorophyll A - chemistry
Chlorophyll A - isolation & purification
Chlorophyll A - pharmacology
Cytokines - analysis
Cytokines - metabolism
Dulse
Liquid-Liquid Extraction
Macrophages - drug effects
Macrophages - metabolism
Mice
Pheophorbide
Phycobiliprotein
Phycobiliproteins - chemistry
Phycobiliproteins - isolation & purification
Phycobiliproteins - pharmacology
Plant Extracts - chemistry
Plant Extracts - pharmacology
RAW 264.7 Cells
Rhodophyta - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Water-extract
title Anti-inflammatory effects of dulse (Palmaria palmata) resulting from the simultaneous water-extraction of phycobiliproteins and chlorophyll a
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