Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia
Background Reliable nuclear immunohistochemical stains for sebaceous neoplasms have not been readily available. Positive nuclear staining has been reported for GATA3 and factor XIIIa (AC‐1A1). We sought to determine the diagnostic utility of these nuclear stains by comparing their staining pattern t...
Gespeichert in:
| Veröffentlicht in: | Journal of cutaneous pathology 2018-01, Vol.45 (1), p.1-7 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7 |
|---|---|
| container_issue | 1 |
| container_start_page | 1 |
| container_title | Journal of cutaneous pathology |
| container_volume | 45 |
| creator | Tjarks, Brian J. Pownell, Brandy R. Evans, Claire Thompson, Paul A. Kerkvliet, Amy M. Koch, Michael R. D. Jassim, Ali D. |
| description | Background
Reliable nuclear immunohistochemical stains for sebaceous neoplasms have not been readily available. Positive nuclear staining has been reported for GATA3 and factor XIIIa (AC‐1A1). We sought to determine the diagnostic utility of these nuclear stains by comparing their staining pattern to adipophilin, a consistently positive cytoplasmic stain.
Methods
Cases with the diagnosis of sebaceous hyperplasia, sebaceous adenoma, sebaceous epithelioma/sebaceoma, sebaceous carcinoma, and nonsebaceous neoplasms (basal cell carcinoma and squamous cell carcinoma) were examined. Intensity and extent of staining of the basal cells and mature sebocytes were evaluated for each stain.
Results
Factor XIIIa (AC‐1A1) was 87.3% sensitive and 95.1% specific for all sebaceous neoplasms sand showed high inter‐observer reliability. Adipophilin was 83.2% sensitive and 87.8% specific. GATA3 was the least sensitive (80.9%) and specific (75.6%) marker. When factor XIIIa was compared against composite staining of all three markers its staining was still uniquely significant (P = .0210).
Conclusion
Factor XIIIa (AC‐1A1) is a sensitive and specific nuclear marker for sebaceous differentiation. Its diagnostic utility exceeds that of adipophilin. Factor XIIIa should be included in the expanding group of immunohistochemical and special stains which can be utilized to aid in the diagnosis of sebaceous neoplasms. |
| doi_str_mv | 10.1111/cup.13037 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1936258901</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1936258901</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3537-7b62f4dd3bacf9004b2e1f92b1e27fee758c21ec79e0d1c226657e9c25e329b73</originalsourceid><addsrcrecordid>eNp1kc1q3DAQx0VpyG7SHPoCRZBLAnVWH2t9HM2SpAuB9JCF3owsjxMtXsuV7ITc-gh9xjxJtHXaQ6BzGWb48WOYP0KfKbmgqRZ27C8oJ1x-QHMqCMlILshHNCdplwmt5AwdxbglhAol8kM0Y0pJzpZyjp4uH007msH5DpuuxtbvehNcTKNvcByM61x3j3szDBC6uF82xg4-4B_r9drgs2L18us3Lej5V2xq1_v-wbVucl0XdwXHaYhQGQt-jLgD37cmOvMJHTSmjXDy1o_R5urybvUtu7m9Xq-Km8zynMtMVoI1y7rmSdBoQpYVA9poVlFgsgGQubKMgpUaSE0tY0LkErRlOXCmK8mP0dnk7YP_OUIcyp2LFtrWdPuDSqq5YLnShCb09B269WPo0nWJkmqphFI6UecTZYOPMUBT9sHtTHguKSn3aZQpjfJPGon98mYcqx3U_8i_70_AYgKeXAvP_zeVq833SfkKcleTQw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1978486889</pqid></control><display><type>article</type><title>Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Tjarks, Brian J. ; Pownell, Brandy R. ; Evans, Claire ; Thompson, Paul A. ; Kerkvliet, Amy M. ; Koch, Michael R. D. ; Jassim, Ali D.</creator><creatorcontrib>Tjarks, Brian J. ; Pownell, Brandy R. ; Evans, Claire ; Thompson, Paul A. ; Kerkvliet, Amy M. ; Koch, Michael R. D. ; Jassim, Ali D.</creatorcontrib><description>Background
Reliable nuclear immunohistochemical stains for sebaceous neoplasms have not been readily available. Positive nuclear staining has been reported for GATA3 and factor XIIIa (AC‐1A1). We sought to determine the diagnostic utility of these nuclear stains by comparing their staining pattern to adipophilin, a consistently positive cytoplasmic stain.
Methods
Cases with the diagnosis of sebaceous hyperplasia, sebaceous adenoma, sebaceous epithelioma/sebaceoma, sebaceous carcinoma, and nonsebaceous neoplasms (basal cell carcinoma and squamous cell carcinoma) were examined. Intensity and extent of staining of the basal cells and mature sebocytes were evaluated for each stain.
Results
Factor XIIIa (AC‐1A1) was 87.3% sensitive and 95.1% specific for all sebaceous neoplasms sand showed high inter‐observer reliability. Adipophilin was 83.2% sensitive and 87.8% specific. GATA3 was the least sensitive (80.9%) and specific (75.6%) marker. When factor XIIIa was compared against composite staining of all three markers its staining was still uniquely significant (P = .0210).
Conclusion
Factor XIIIa (AC‐1A1) is a sensitive and specific nuclear marker for sebaceous differentiation. Its diagnostic utility exceeds that of adipophilin. Factor XIIIa should be included in the expanding group of immunohistochemical and special stains which can be utilized to aid in the diagnosis of sebaceous neoplasms.</description><identifier>ISSN: 0303-6987</identifier><identifier>EISSN: 1600-0560</identifier><identifier>DOI: 10.1111/cup.13037</identifier><identifier>PMID: 28873247</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenoma ; Basal cell carcinoma ; Basal cells ; Biomarkers, Tumor - analysis ; Diagnosis ; Factor XIIIa - analysis ; GATA-3 protein ; GATA3 Transcription Factor - analysis ; Humans ; Hyperplasia ; Immunohistochemistry ; nonmelanoma skin cancer ; Perilipin-2 - analysis ; sebaceous ; Sebaceous Gland Neoplasms - diagnosis ; Sensitivity and Specificity ; Squamous cell carcinoma ; Staining and Labeling ; Tumors</subject><ispartof>Journal of cutaneous pathology, 2018-01, Vol.45 (1), p.1-7</ispartof><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-7b62f4dd3bacf9004b2e1f92b1e27fee758c21ec79e0d1c226657e9c25e329b73</citedby><cites>FETCH-LOGICAL-c3537-7b62f4dd3bacf9004b2e1f92b1e27fee758c21ec79e0d1c226657e9c25e329b73</cites><orcidid>0000-0002-8538-8491</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fcup.13037$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fcup.13037$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28873247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tjarks, Brian J.</creatorcontrib><creatorcontrib>Pownell, Brandy R.</creatorcontrib><creatorcontrib>Evans, Claire</creatorcontrib><creatorcontrib>Thompson, Paul A.</creatorcontrib><creatorcontrib>Kerkvliet, Amy M.</creatorcontrib><creatorcontrib>Koch, Michael R. D.</creatorcontrib><creatorcontrib>Jassim, Ali D.</creatorcontrib><title>Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia</title><title>Journal of cutaneous pathology</title><addtitle>J Cutan Pathol</addtitle><description>Background
Reliable nuclear immunohistochemical stains for sebaceous neoplasms have not been readily available. Positive nuclear staining has been reported for GATA3 and factor XIIIa (AC‐1A1). We sought to determine the diagnostic utility of these nuclear stains by comparing their staining pattern to adipophilin, a consistently positive cytoplasmic stain.
Methods
Cases with the diagnosis of sebaceous hyperplasia, sebaceous adenoma, sebaceous epithelioma/sebaceoma, sebaceous carcinoma, and nonsebaceous neoplasms (basal cell carcinoma and squamous cell carcinoma) were examined. Intensity and extent of staining of the basal cells and mature sebocytes were evaluated for each stain.
Results
Factor XIIIa (AC‐1A1) was 87.3% sensitive and 95.1% specific for all sebaceous neoplasms sand showed high inter‐observer reliability. Adipophilin was 83.2% sensitive and 87.8% specific. GATA3 was the least sensitive (80.9%) and specific (75.6%) marker. When factor XIIIa was compared against composite staining of all three markers its staining was still uniquely significant (P = .0210).
Conclusion
Factor XIIIa (AC‐1A1) is a sensitive and specific nuclear marker for sebaceous differentiation. Its diagnostic utility exceeds that of adipophilin. Factor XIIIa should be included in the expanding group of immunohistochemical and special stains which can be utilized to aid in the diagnosis of sebaceous neoplasms.</description><subject>Adenoma</subject><subject>Basal cell carcinoma</subject><subject>Basal cells</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Diagnosis</subject><subject>Factor XIIIa - analysis</subject><subject>GATA-3 protein</subject><subject>GATA3 Transcription Factor - analysis</subject><subject>Humans</subject><subject>Hyperplasia</subject><subject>Immunohistochemistry</subject><subject>nonmelanoma skin cancer</subject><subject>Perilipin-2 - analysis</subject><subject>sebaceous</subject><subject>Sebaceous Gland Neoplasms - diagnosis</subject><subject>Sensitivity and Specificity</subject><subject>Squamous cell carcinoma</subject><subject>Staining and Labeling</subject><subject>Tumors</subject><issn>0303-6987</issn><issn>1600-0560</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1q3DAQx0VpyG7SHPoCRZBLAnVWH2t9HM2SpAuB9JCF3owsjxMtXsuV7ITc-gh9xjxJtHXaQ6BzGWb48WOYP0KfKbmgqRZ27C8oJ1x-QHMqCMlILshHNCdplwmt5AwdxbglhAol8kM0Y0pJzpZyjp4uH007msH5DpuuxtbvehNcTKNvcByM61x3j3szDBC6uF82xg4-4B_r9drgs2L18us3Lej5V2xq1_v-wbVucl0XdwXHaYhQGQt-jLgD37cmOvMJHTSmjXDy1o_R5urybvUtu7m9Xq-Km8zynMtMVoI1y7rmSdBoQpYVA9poVlFgsgGQubKMgpUaSE0tY0LkErRlOXCmK8mP0dnk7YP_OUIcyp2LFtrWdPuDSqq5YLnShCb09B269WPo0nWJkmqphFI6UecTZYOPMUBT9sHtTHguKSn3aZQpjfJPGon98mYcqx3U_8i_70_AYgKeXAvP_zeVq833SfkKcleTQw</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Tjarks, Brian J.</creator><creator>Pownell, Brandy R.</creator><creator>Evans, Claire</creator><creator>Thompson, Paul A.</creator><creator>Kerkvliet, Amy M.</creator><creator>Koch, Michael R. D.</creator><creator>Jassim, Ali D.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8538-8491</orcidid></search><sort><creationdate>201801</creationdate><title>Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia</title><author>Tjarks, Brian J. ; Pownell, Brandy R. ; Evans, Claire ; Thompson, Paul A. ; Kerkvliet, Amy M. ; Koch, Michael R. D. ; Jassim, Ali D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-7b62f4dd3bacf9004b2e1f92b1e27fee758c21ec79e0d1c226657e9c25e329b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenoma</topic><topic>Basal cell carcinoma</topic><topic>Basal cells</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Diagnosis</topic><topic>Factor XIIIa - analysis</topic><topic>GATA-3 protein</topic><topic>GATA3 Transcription Factor - analysis</topic><topic>Humans</topic><topic>Hyperplasia</topic><topic>Immunohistochemistry</topic><topic>nonmelanoma skin cancer</topic><topic>Perilipin-2 - analysis</topic><topic>sebaceous</topic><topic>Sebaceous Gland Neoplasms - diagnosis</topic><topic>Sensitivity and Specificity</topic><topic>Squamous cell carcinoma</topic><topic>Staining and Labeling</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tjarks, Brian J.</creatorcontrib><creatorcontrib>Pownell, Brandy R.</creatorcontrib><creatorcontrib>Evans, Claire</creatorcontrib><creatorcontrib>Thompson, Paul A.</creatorcontrib><creatorcontrib>Kerkvliet, Amy M.</creatorcontrib><creatorcontrib>Koch, Michael R. D.</creatorcontrib><creatorcontrib>Jassim, Ali D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cutaneous pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tjarks, Brian J.</au><au>Pownell, Brandy R.</au><au>Evans, Claire</au><au>Thompson, Paul A.</au><au>Kerkvliet, Amy M.</au><au>Koch, Michael R. D.</au><au>Jassim, Ali D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia</atitle><jtitle>Journal of cutaneous pathology</jtitle><addtitle>J Cutan Pathol</addtitle><date>2018-01</date><risdate>2018</risdate><volume>45</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0303-6987</issn><eissn>1600-0560</eissn><abstract>Background
Reliable nuclear immunohistochemical stains for sebaceous neoplasms have not been readily available. Positive nuclear staining has been reported for GATA3 and factor XIIIa (AC‐1A1). We sought to determine the diagnostic utility of these nuclear stains by comparing their staining pattern to adipophilin, a consistently positive cytoplasmic stain.
Methods
Cases with the diagnosis of sebaceous hyperplasia, sebaceous adenoma, sebaceous epithelioma/sebaceoma, sebaceous carcinoma, and nonsebaceous neoplasms (basal cell carcinoma and squamous cell carcinoma) were examined. Intensity and extent of staining of the basal cells and mature sebocytes were evaluated for each stain.
Results
Factor XIIIa (AC‐1A1) was 87.3% sensitive and 95.1% specific for all sebaceous neoplasms sand showed high inter‐observer reliability. Adipophilin was 83.2% sensitive and 87.8% specific. GATA3 was the least sensitive (80.9%) and specific (75.6%) marker. When factor XIIIa was compared against composite staining of all three markers its staining was still uniquely significant (P = .0210).
Conclusion
Factor XIIIa (AC‐1A1) is a sensitive and specific nuclear marker for sebaceous differentiation. Its diagnostic utility exceeds that of adipophilin. Factor XIIIa should be included in the expanding group of immunohistochemical and special stains which can be utilized to aid in the diagnosis of sebaceous neoplasms.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>28873247</pmid><doi>10.1111/cup.13037</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-8538-8491</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0303-6987 |
| ispartof | Journal of cutaneous pathology, 2018-01, Vol.45 (1), p.1-7 |
| issn | 0303-6987 1600-0560 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1936258901 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adenoma Basal cell carcinoma Basal cells Biomarkers, Tumor - analysis Diagnosis Factor XIIIa - analysis GATA-3 protein GATA3 Transcription Factor - analysis Humans Hyperplasia Immunohistochemistry nonmelanoma skin cancer Perilipin-2 - analysis sebaceous Sebaceous Gland Neoplasms - diagnosis Sensitivity and Specificity Squamous cell carcinoma Staining and Labeling Tumors |
| title | Evaluation and comparison of staining patterns of factor XIIIa (AC‐1A1), adipophilin and GATA3 in sebaceous neoplasia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T17%3A02%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20and%20comparison%20of%20staining%20patterns%20of%20factor%20XIIIa%20(AC%E2%80%901A1),%20adipophilin%20and%20GATA3%20in%20sebaceous%20neoplasia&rft.jtitle=Journal%20of%20cutaneous%20pathology&rft.au=Tjarks,%20Brian%20J.&rft.date=2018-01&rft.volume=45&rft.issue=1&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.issn=0303-6987&rft.eissn=1600-0560&rft_id=info:doi/10.1111/cup.13037&rft_dat=%3Cproquest_cross%3E1936258901%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1978486889&rft_id=info:pmid/28873247&rfr_iscdi=true |