Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model
While silicon nitride (Si3N4) is an antimicrobial and osseointegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin‐resistant strain of Staphylococcus aureus (MRSA), this study evaluated Si3N4 implants in vitro utilizing scanning...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2017-12, Vol.105 (12), p.3413-3421 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Ishikawa, Masahiro de Mesy Bentley, Karen L. McEntire, Bryan J. Bal, B. Sonny Schwarz, Edward M. Xie, Chao |
| description | While silicon nitride (Si3N4) is an antimicrobial and osseointegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin‐resistant strain of Staphylococcus aureus (MRSA), this study evaluated Si3N4 implants in vitro utilizing scanning electron microscopy (SEM) with colony forming unit (CFU) assays, and later in an established in vivo murine tibia model of implant‐associated osteomyelitis. In vitro, the “as‐fired” Si3N4 implants displayed significant reductions in adherent bacteria versus machined Si3N4 (2.6 × 104 vs. 8.7 × 104 CFU, respectively; p |
| doi_str_mv | 10.1002/jbm.a.36189 |
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Sonny ; Schwarz, Edward M. ; Xie, Chao</creator><creatorcontrib>Ishikawa, Masahiro ; de Mesy Bentley, Karen L. ; McEntire, Bryan J. ; Bal, B. Sonny ; Schwarz, Edward M. ; Xie, Chao</creatorcontrib><description>While silicon nitride (Si3N4) is an antimicrobial and osseointegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin‐resistant strain of Staphylococcus aureus (MRSA), this study evaluated Si3N4 implants in vitro utilizing scanning electron microscopy (SEM) with colony forming unit (CFU) assays, and later in an established in vivo murine tibia model of implant‐associated osteomyelitis. In vitro, the “as‐fired” Si3N4 implants displayed significant reductions in adherent bacteria versus machined Si3N4 (2.6 × 104 vs. 8.7 × 104 CFU, respectively; p < 0.0002). Moreover, SEM imaging demonstrated that MRSA cannot directly adhere to native as‐fired Si3N4. Subsequently, a cross‐sectional study was completed in which sterile or MRSA contaminated as‐fired and machined Si3N4 implants were inserted into the tibiae of 8‐week old female Balb/c mice, and harvested on day 1, 3, 5, 7, 10, or 14 post‐operatively for SEM. The findings demonstrated that the antimicrobial activity of the as‐fired implants resulted from macrophage clearance of the bacteria during biofilm formation on day 1, followed by osseointegration through the apparent recruitment of mesenchymal stem cells on days 3–5, which differentiated into osteoblasts on days 7–14. In contrast, the antimicrobial behavior of the machined Si3N4 was due to repulsion of the bacteria, a phenomenon that also limited osteogenesis, as host cells were also unable to adhere to the machined surface. Taken together, these results suggest that the in vivo biological behavior of Si3N4 orthopaedic implants is driven by critical features of their surface nanotopography. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3413–3421, 2017.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.36189</identifier><identifier>PMID: 28865177</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animal models ; Antiinfectives and antibacterials ; antimicrobial ; Antimicrobial activity ; Antimicrobial agents ; Bacteria ; Biocompatibility ; Biofilms ; Biological effects ; Biomedical materials ; Bone implants ; Drug resistance ; Electron microscopy ; Macrophages ; Mesenchyme ; Methicillin ; Orthopaedic implants ; Osseointegration ; osseointegrative ; Osteoblasts ; Osteogenesis ; Osteomyelitis ; Recruitment ; Scanning electron microscopy ; Scanning transmission electron microscopy ; Silicon ; Silicon nitride ; Staphylococcus aureus ; Staphylococcus infections ; Stem cell transplantation ; Stem cells ; Surgical implants ; Tibia ; Topography ; Transplants & implants</subject><ispartof>Journal of biomedical materials research. 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Sonny</creatorcontrib><creatorcontrib>Schwarz, Edward M.</creatorcontrib><creatorcontrib>Xie, Chao</creatorcontrib><title>Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model</title><title>Journal of biomedical materials research. Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>While silicon nitride (Si3N4) is an antimicrobial and osseointegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin‐resistant strain of Staphylococcus aureus (MRSA), this study evaluated Si3N4 implants in vitro utilizing scanning electron microscopy (SEM) with colony forming unit (CFU) assays, and later in an established in vivo murine tibia model of implant‐associated osteomyelitis. In vitro, the “as‐fired” Si3N4 implants displayed significant reductions in adherent bacteria versus machined Si3N4 (2.6 × 104 vs. 8.7 × 104 CFU, respectively; p < 0.0002). Moreover, SEM imaging demonstrated that MRSA cannot directly adhere to native as‐fired Si3N4. Subsequently, a cross‐sectional study was completed in which sterile or MRSA contaminated as‐fired and machined Si3N4 implants were inserted into the tibiae of 8‐week old female Balb/c mice, and harvested on day 1, 3, 5, 7, 10, or 14 post‐operatively for SEM. The findings demonstrated that the antimicrobial activity of the as‐fired implants resulted from macrophage clearance of the bacteria during biofilm formation on day 1, followed by osseointegration through the apparent recruitment of mesenchymal stem cells on days 3–5, which differentiated into osteoblasts on days 7–14. In contrast, the antimicrobial behavior of the machined Si3N4 was due to repulsion of the bacteria, a phenomenon that also limited osteogenesis, as host cells were also unable to adhere to the machined surface. Taken together, these results suggest that the in vivo biological behavior of Si3N4 orthopaedic implants is driven by critical features of their surface nanotopography. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3413–3421, 2017.</description><subject>Animal models</subject><subject>Antiinfectives and antibacterials</subject><subject>antimicrobial</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Biofilms</subject><subject>Biological effects</subject><subject>Biomedical materials</subject><subject>Bone implants</subject><subject>Drug resistance</subject><subject>Electron microscopy</subject><subject>Macrophages</subject><subject>Mesenchyme</subject><subject>Methicillin</subject><subject>Orthopaedic implants</subject><subject>Osseointegration</subject><subject>osseointegrative</subject><subject>Osteoblasts</subject><subject>Osteogenesis</subject><subject>Osteomyelitis</subject><subject>Recruitment</subject><subject>Scanning electron microscopy</subject><subject>Scanning transmission electron microscopy</subject><subject>Silicon</subject><subject>Silicon nitride</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Surgical implants</subject><subject>Tibia</subject><subject>Topography</subject><subject>Transplants & implants</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kTtvFDEURi0EIiFQ0SNLNEhoFtvjx7oMEU8FUQC1ZXuuwauZ8WB7iLbjp8ebDSkoUvlaOvfo0_0Qek7JhhLC3uzctLGbXtKtfoBOqRCs41qKh4eZ665nWp6gJ6XsGiyJYI_RCdtupaBKnaK_39YcrAdc05J-Zrv82uMUcIlj9GnGc6w5DoBtCOBrwXaucYo-Jxft2H4DTqVAinOFtlzjH8BLTgvkGqEcRDXekHFaxrZbcJyxxdOa4wx4SgOMT9GjYMcCz27fM_Tj_bvvFx-7y68fPl2cX3aey153zlIXNCNcKKK5FQ6GIYD01gnQTHiuXE_UwAdJAneSBeuUUtpL66VSAP0ZenX0tny_VyjVTLF4GFssSGsxVLcLyp5q1tCX_6G7tOa5pWuUkC2DFLxRr49Uu0YpGYJZcpxs3htKzKEY04ox1twU0-gXt87VTTDcsf-aaAA7AldxhP19LvP57Zfzo_UaJc6cGA</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Ishikawa, Masahiro</creator><creator>de Mesy Bentley, Karen L.</creator><creator>McEntire, Bryan J.</creator><creator>Bal, B. Sonny</creator><creator>Schwarz, Edward M.</creator><creator>Xie, Chao</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7612-9789</orcidid></search><sort><creationdate>201712</creationdate><title>Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model</title><author>Ishikawa, Masahiro ; de Mesy Bentley, Karen L. ; McEntire, Bryan J. ; Bal, B. Sonny ; Schwarz, Edward M. ; Xie, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4639-ba1bf920457094a5beddfe6cab5e925c47b307d4d60f4b62fab7779c6ac677ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal models</topic><topic>Antiinfectives and antibacterials</topic><topic>antimicrobial</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Biocompatibility</topic><topic>Biofilms</topic><topic>Biological effects</topic><topic>Biomedical materials</topic><topic>Bone implants</topic><topic>Drug resistance</topic><topic>Electron microscopy</topic><topic>Macrophages</topic><topic>Mesenchyme</topic><topic>Methicillin</topic><topic>Orthopaedic implants</topic><topic>Osseointegration</topic><topic>osseointegrative</topic><topic>Osteoblasts</topic><topic>Osteogenesis</topic><topic>Osteomyelitis</topic><topic>Recruitment</topic><topic>Scanning electron microscopy</topic><topic>Scanning transmission electron microscopy</topic><topic>Silicon</topic><topic>Silicon nitride</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Surgical implants</topic><topic>Tibia</topic><topic>Topography</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishikawa, Masahiro</creatorcontrib><creatorcontrib>de Mesy Bentley, Karen L.</creatorcontrib><creatorcontrib>McEntire, Bryan J.</creatorcontrib><creatorcontrib>Bal, B. 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishikawa, Masahiro</au><au>de Mesy Bentley, Karen L.</au><au>McEntire, Bryan J.</au><au>Bal, B. Sonny</au><au>Schwarz, Edward M.</au><au>Xie, Chao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2017-12</date><risdate>2017</risdate><volume>105</volume><issue>12</issue><spage>3413</spage><epage>3421</epage><pages>3413-3421</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>While silicon nitride (Si3N4) is an antimicrobial and osseointegrative orthopaedic biomaterial, the contribution of surface topography to these properties is unknown. Using a methicillin‐resistant strain of Staphylococcus aureus (MRSA), this study evaluated Si3N4 implants in vitro utilizing scanning electron microscopy (SEM) with colony forming unit (CFU) assays, and later in an established in vivo murine tibia model of implant‐associated osteomyelitis. In vitro, the “as‐fired” Si3N4 implants displayed significant reductions in adherent bacteria versus machined Si3N4 (2.6 × 104 vs. 8.7 × 104 CFU, respectively; p < 0.0002). Moreover, SEM imaging demonstrated that MRSA cannot directly adhere to native as‐fired Si3N4. Subsequently, a cross‐sectional study was completed in which sterile or MRSA contaminated as‐fired and machined Si3N4 implants were inserted into the tibiae of 8‐week old female Balb/c mice, and harvested on day 1, 3, 5, 7, 10, or 14 post‐operatively for SEM. The findings demonstrated that the antimicrobial activity of the as‐fired implants resulted from macrophage clearance of the bacteria during biofilm formation on day 1, followed by osseointegration through the apparent recruitment of mesenchymal stem cells on days 3–5, which differentiated into osteoblasts on days 7–14. In contrast, the antimicrobial behavior of the machined Si3N4 was due to repulsion of the bacteria, a phenomenon that also limited osteogenesis, as host cells were also unable to adhere to the machined surface. Taken together, these results suggest that the in vivo biological behavior of Si3N4 orthopaedic implants is driven by critical features of their surface nanotopography. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3413–3421, 2017.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28865177</pmid><doi>10.1002/jbm.a.36189</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7612-9789</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animal models Antiinfectives and antibacterials antimicrobial Antimicrobial activity Antimicrobial agents Bacteria Biocompatibility Biofilms Biological effects Biomedical materials Bone implants Drug resistance Electron microscopy Macrophages Mesenchyme Methicillin Orthopaedic implants Osseointegration osseointegrative Osteoblasts Osteogenesis Osteomyelitis Recruitment Scanning electron microscopy Scanning transmission electron microscopy Silicon Silicon nitride Staphylococcus aureus Staphylococcus infections Stem cell transplantation Stem cells Surgical implants Tibia Topography Transplants & implants |
| title | Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model |
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