Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique

An isotope-dilution mass spectrometry (IDMS) method for measuring insulin levels in human serum was developed using C-terminal-derivatization method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The carboxyl groups of Glu-C-cleavage products were derivatized with 1-(2-pyrim...

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Veröffentlicht in:	Analytical biochemistry 2017-11, Vol.537, p.26-32
Hauptverfasser:	Sakaguchi, Yohei, Kinumi, Tomoya, Takatsu, Akiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, High Pressure Liquid Derivatization Deuterium - chemistry Human insulin Humans IDMS Insulins - blood Insulins - chemistry Insulins - metabolism Isotope Labeling LC-MS/MS Peptides - blood Peptides - chemistry Peptides - metabolism Sensitive Serine Endopeptidases - metabolism Tandem Mass Spectrometry
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