Triterpenoids Isolated from Alnus japonica Inhibited LPS-Induced Inflammatory Mediators in HT-29 Cells and RAW264.7 Cells
Alnus japonica (Betulaceae) is a broad-leaved tree easily found in damp regions within the mountains of Korea and Japan. Four triterpenoids (1–4) from the fruits of A. japonica, including the newly isolated 3β-hydroxy-lanost-9(11),23(24)-dien-25,26-diol (3), inhibited the lipopolysaccharide (LPS)-in...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2017/09/01, Vol.40(9), pp.1544-1550 |
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| description | Alnus japonica (Betulaceae) is a broad-leaved tree easily found in damp regions within the mountains of Korea and Japan. Four triterpenoids (1–4) from the fruits of A. japonica, including the newly isolated 3β-hydroxy-lanost-9(11),23(24)-dien-25,26-diol (3), inhibited the lipopolysaccharide (LPS)-induced expression of the chemotactic cytokine interleukin (IL)-8 and nitric oxide (NO) production in HT-29 colon epithelial cells and RAW264.7 macrophages, respectively. Among these triterpenoids, compound 4, which showed the most potent inhibitory activity, effectively down-regulated LPS-induced protein expression of inducible nitric oxide synthase (iNOS) in RAW264.7 cells and in HT-29 cells. Also, compound 4 concentration-dependently inhibited the levels of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 in macrophage cells. These triterpenoids isolated from A. japonica fruits are thought to contribute to the anti-inflammatory activities of macrophage and colon epithelial cells, which are important for regulating the colon immune system. They are expected to be potential candidates for therapeutic agents against inflammatory bowel disease. |
| doi_str_mv | 10.1248/bpb.b16-00895 |
| format | Article |
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Four triterpenoids (1–4) from the fruits of A. japonica, including the newly isolated 3β-hydroxy-lanost-9(11),23(24)-dien-25,26-diol (3), inhibited the lipopolysaccharide (LPS)-induced expression of the chemotactic cytokine interleukin (IL)-8 and nitric oxide (NO) production in HT-29 colon epithelial cells and RAW264.7 macrophages, respectively. Among these triterpenoids, compound 4, which showed the most potent inhibitory activity, effectively down-regulated LPS-induced protein expression of inducible nitric oxide synthase (iNOS) in RAW264.7 cells and in HT-29 cells. Also, compound 4 concentration-dependently inhibited the levels of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 in macrophage cells. These triterpenoids isolated from A. japonica fruits are thought to contribute to the anti-inflammatory activities of macrophage and colon epithelial cells, which are important for regulating the colon immune system. They are expected to be potential candidates for therapeutic agents against inflammatory bowel disease.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b16-00895</identifier><identifier>PMID: 28867736</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Alnus - chemistry ; Alnus japonica ; Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Chemical compounds ; Colon ; Cytokines - biosynthesis ; Epithelial cells ; Fruit - chemistry ; Fruits ; HT-29 cell ; HT29 Cells ; Humans ; Immune system ; inflammatory bowel disease (IBD) ; Inflammatory bowel diseases ; Inflammatory Bowel Diseases - drug therapy ; Interleukin 6 ; Interleukin-6 - biosynthesis ; Intestine ; Lipopolysaccharides ; Lipopolysaccharides - antagonists & inhibitors ; Macrophages ; Mice ; Mountains ; Nitric oxide ; Nitric Oxide - metabolism ; Nitric Oxide Synthase Type II - antagonists & inhibitors ; Nitric-oxide synthase ; RAW 264.7 Cells ; RAW264.7 cell ; Structure-Activity Relationship ; Triterpenes - isolation & purification ; Triterpenes - pharmacology ; triterpenoid ; Triterpenoids</subject><ispartof>Biological and Pharmaceutical Bulletin, 2017/09/01, Vol.40(9), pp.1544-1550</ispartof><rights>2017 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c702t-e45623f636318f7e8e1a222f87a19aa88971c97086a5c811935c5eba5c5678093</citedby><cites>FETCH-LOGICAL-c702t-e45623f636318f7e8e1a222f87a19aa88971c97086a5c811935c5eba5c5678093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,1885,4026,27930,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28867736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Mina</creatorcontrib><creatorcontrib>Shim, Sun-Yup</creatorcontrib><creatorcontrib>Sung, Sang Hyun</creatorcontrib><creatorcontrib>aCollege of Pharmacy</creatorcontrib><creatorcontrib>bSuncheon Research Center for Natural Medicines</creatorcontrib><creatorcontrib>Seoul National University</creatorcontrib><creatorcontrib>Sunchon National University</creatorcontrib><creatorcontrib>cResearch Institute of Life and Pharmaceutical Sciences</creatorcontrib><creatorcontrib>dCollege of Pharmacy and Research Institute of Pharmaceutical Sciences</creatorcontrib><title>Triterpenoids Isolated from Alnus japonica Inhibited LPS-Induced Inflammatory Mediators in HT-29 Cells and RAW264.7 Cells</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Alnus japonica (Betulaceae) is a broad-leaved tree easily found in damp regions within the mountains of Korea and Japan. Four triterpenoids (1–4) from the fruits of A. japonica, including the newly isolated 3β-hydroxy-lanost-9(11),23(24)-dien-25,26-diol (3), inhibited the lipopolysaccharide (LPS)-induced expression of the chemotactic cytokine interleukin (IL)-8 and nitric oxide (NO) production in HT-29 colon epithelial cells and RAW264.7 macrophages, respectively. Among these triterpenoids, compound 4, which showed the most potent inhibitory activity, effectively down-regulated LPS-induced protein expression of inducible nitric oxide synthase (iNOS) in RAW264.7 cells and in HT-29 cells. Also, compound 4 concentration-dependently inhibited the levels of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 in macrophage cells. These triterpenoids isolated from A. japonica fruits are thought to contribute to the anti-inflammatory activities of macrophage and colon epithelial cells, which are important for regulating the colon immune system. They are expected to be potential candidates for therapeutic agents against inflammatory bowel disease.</description><subject>Alnus - chemistry</subject><subject>Alnus japonica</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Chemical compounds</subject><subject>Colon</subject><subject>Cytokines - biosynthesis</subject><subject>Epithelial cells</subject><subject>Fruit - chemistry</subject><subject>Fruits</subject><subject>HT-29 cell</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Immune system</subject><subject>inflammatory bowel disease (IBD)</subject><subject>Inflammatory bowel diseases</subject><subject>Inflammatory Bowel Diseases - drug therapy</subject><subject>Interleukin 6</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Intestine</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - antagonists & inhibitors</subject><subject>Macrophages</subject><subject>Mice</subject><subject>Mountains</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Synthase Type II - antagonists & inhibitors</subject><subject>Nitric-oxide synthase</subject><subject>RAW 264.7 Cells</subject><subject>RAW264.7 cell</subject><subject>Structure-Activity Relationship</subject><subject>Triterpenes - isolation & purification</subject><subject>Triterpenes - pharmacology</subject><subject>triterpenoid</subject><subject>Triterpenoids</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2P0zAQhiMEYsvCkSuyxIVLij_ir2NVLbuRikBQxNFyHId1ldjBTg799zjNUiQu47Hn8cxrv0XxFsEtwpX42IzNtkGshFBI-qzYIFLxkmJEnxcbKJEoGaLipniV0glCyCEmL4sbLATjnLBNcT5GN9k4Wh9cm0CdQq8n24IuhgHsej8ncNJj8M5oUPtH17ilevj6vax9O5uc177r9TDoKcQz-Gxbt2QJOA8ejiWWYG_7PgHtW_Bt9xOzasvXo9fFi073yb55Wm-LH5_ujvuH8vDlvt7vDqXJYqfSVpRh0jHCCBIdt8IijTHuBNdIai2E5MhIDgXT1AiEJKGG2iZvKOMCSnJbfFj7jjH8nm2a1OCSyQq0t2FOarlBJKQEZfT9f-gpzNFndReKcSQgy1S5UiaGlKLt1BjdoONZIagWT1T2RGVP1MWTzL976jo3g22v9F8TMnC_Arma_7kPvnfe_pttEm9c6IPCEPHctIJQKoiFQrSqcqAQS0opWx6wXzud0qR_2esoHSdnensRVkEll3AVeK2aRx2V9eQPoMazFw</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>Lee, Mina</creator><creator>Shim, Sun-Yup</creator><creator>Sung, Sang Hyun</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>Triterpenoids Isolated from Alnus japonica Inhibited LPS-Induced Inflammatory Mediators in HT-29 Cells and RAW264.7 Cells</title><author>Lee, Mina ; Shim, Sun-Yup ; Sung, Sang Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c702t-e45623f636318f7e8e1a222f87a19aa88971c97086a5c811935c5eba5c5678093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alnus - chemistry</topic><topic>Alnus japonica</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Chemical compounds</topic><topic>Colon</topic><topic>Cytokines - biosynthesis</topic><topic>Epithelial cells</topic><topic>Fruit - chemistry</topic><topic>Fruits</topic><topic>HT-29 cell</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Immune system</topic><topic>inflammatory bowel disease (IBD)</topic><topic>Inflammatory bowel diseases</topic><topic>Inflammatory Bowel Diseases - drug therapy</topic><topic>Interleukin 6</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Intestine</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - antagonists & inhibitors</topic><topic>Macrophages</topic><topic>Mice</topic><topic>Mountains</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Synthase Type II - antagonists & inhibitors</topic><topic>Nitric-oxide synthase</topic><topic>RAW 264.7 Cells</topic><topic>RAW264.7 cell</topic><topic>Structure-Activity Relationship</topic><topic>Triterpenes - isolation & purification</topic><topic>Triterpenes - pharmacology</topic><topic>triterpenoid</topic><topic>Triterpenoids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Mina</creatorcontrib><creatorcontrib>Shim, Sun-Yup</creatorcontrib><creatorcontrib>Sung, Sang Hyun</creatorcontrib><creatorcontrib>aCollege of Pharmacy</creatorcontrib><creatorcontrib>bSuncheon Research Center for Natural Medicines</creatorcontrib><creatorcontrib>Seoul National University</creatorcontrib><creatorcontrib>Sunchon National University</creatorcontrib><creatorcontrib>cResearch Institute of Life and Pharmaceutical Sciences</creatorcontrib><creatorcontrib>dCollege of Pharmacy and Research Institute of Pharmaceutical Sciences</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Mina</au><au>Shim, Sun-Yup</au><au>Sung, Sang Hyun</au><aucorp>aCollege of Pharmacy</aucorp><aucorp>bSuncheon Research Center for Natural Medicines</aucorp><aucorp>Seoul National University</aucorp><aucorp>Sunchon National University</aucorp><aucorp>cResearch Institute of Life and Pharmaceutical Sciences</aucorp><aucorp>dCollege of Pharmacy and Research Institute of Pharmaceutical Sciences</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triterpenoids Isolated from Alnus japonica Inhibited LPS-Induced Inflammatory Mediators in HT-29 Cells and RAW264.7 Cells</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2017</date><risdate>2017</risdate><volume>40</volume><issue>9</issue><spage>1544</spage><epage>1550</epage><pages>1544-1550</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Alnus japonica (Betulaceae) is a broad-leaved tree easily found in damp regions within the mountains of Korea and Japan. Four triterpenoids (1–4) from the fruits of A. japonica, including the newly isolated 3β-hydroxy-lanost-9(11),23(24)-dien-25,26-diol (3), inhibited the lipopolysaccharide (LPS)-induced expression of the chemotactic cytokine interleukin (IL)-8 and nitric oxide (NO) production in HT-29 colon epithelial cells and RAW264.7 macrophages, respectively. Among these triterpenoids, compound 4, which showed the most potent inhibitory activity, effectively down-regulated LPS-induced protein expression of inducible nitric oxide synthase (iNOS) in RAW264.7 cells and in HT-29 cells. Also, compound 4 concentration-dependently inhibited the levels of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 in macrophage cells. These triterpenoids isolated from A. japonica fruits are thought to contribute to the anti-inflammatory activities of macrophage and colon epithelial cells, which are important for regulating the colon immune system. They are expected to be potential candidates for therapeutic agents against inflammatory bowel disease.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>28867736</pmid><doi>10.1248/bpb.b16-00895</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 2017/09/01, Vol.40(9), pp.1544-1550 |
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| subjects | Alnus - chemistry Alnus japonica Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Chemical compounds Colon Cytokines - biosynthesis Epithelial cells Fruit - chemistry Fruits HT-29 cell HT29 Cells Humans Immune system inflammatory bowel disease (IBD) Inflammatory bowel diseases Inflammatory Bowel Diseases - drug therapy Interleukin 6 Interleukin-6 - biosynthesis Intestine Lipopolysaccharides Lipopolysaccharides - antagonists & inhibitors Macrophages Mice Mountains Nitric oxide Nitric Oxide - metabolism Nitric Oxide Synthase Type II - antagonists & inhibitors Nitric-oxide synthase RAW 264.7 Cells RAW264.7 cell Structure-Activity Relationship Triterpenes - isolation & purification Triterpenes - pharmacology triterpenoid Triterpenoids |
| title | Triterpenoids Isolated from Alnus japonica Inhibited LPS-Induced Inflammatory Mediators in HT-29 Cells and RAW264.7 Cells |
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