Comparison of Laboratory‐Developed and Commercial Monoclonal Antibody‐Based Sandwich Enzyme‐Linked Immunosorbent Assays for Almond (Prunus dulcis) Detection and Quantification

A commercially available monoclonal antibody (mAb)‐based direct sandwich enzyme‐linked immunosorbent assay (ELISA) kit (BioFront Technologies, Tallahassee, Fla., U.S.A.) was compared with an in‐house developed mAb 4C10‐based ELISA for almond detection. The assays were comparable in sensitivity (limi...

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Veröffentlicht in:	Journal of food science 2017-10, Vol.82 (10), p.2504-2515
Hauptverfasser:	Liu, Changqi, Chhabra, Guneet S., Zhao, Jing, Zaffran, Valerie D., Gupta, Sahil, Roux, Kenneth H., Gradziel, Thomas M., Sathe, Shridhar K.
Format:	Artikel
Sprache:	eng
Schlagworte:	4C10 Allergens - analysis almond almonds Antibodies, Monoclonal - analysis Antigens Assaying Autoclaving Blanching Consumers cross reaction Cross Reactions Cross-reactivity detection limit ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - economics Enzyme-Linked Immunosorbent Assay - methods Enzymes Food Food industry food matrix Food processing Food processing industry foods Frying Immunoassays Laboratories mAb Monoclonal antibodies MonoTrace Prepared foods Prunus dulcis Prunus dulcis - chemistry Recovery Regulatory agencies Reproducibility Reproducibility of Results roasting Seeds Seeds - chemistry Sensitivity and Specificity
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