Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions
Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at differ...
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| Veröffentlicht in: | Animal science journal 2018-01, Vol.89 (1), p.42-51 |
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| creator | Juanpanich, Theesit Suttirojpattana, Tayita Takayama, Mari Liang, Yuanyuan Dochi, Osamu Parnpai, Rangsun Imai, Kei |
| description | Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG. In experiment 2, intact two‐cell and eight‐cell stage embryos were vitrified in the same solutions used in experiment 1. The EG + PG produced more dead embryos than the EG (P |
| doi_str_mv | 10.1111/asj.12890 |
| format | Article |
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This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG. In experiment 2, intact two‐cell and eight‐cell stage embryos were vitrified in the same solutions used in experiment 1. The EG + PG produced more dead embryos than the EG (P < 0.05). In the EG, the rate of blastocyst formation was similar for the vitrified two‐ and eight‐cell embryos and the non‐vitrified ywo‐cell embryos. In experiment 3, separated blastomeres of two‐ and eight‐cell embryos were vitrified in EG. There was no difference in the rate of blastocyst formation and total number of cells between the two vitrified groups. In summary, at the blastocyst stage, EG + PG was superior, based on both survival rates and cell numbers; however, at the 2–8 cell stage, the use of EG alone was better than the other groups.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/asj.12890</identifier><identifier>PMID: 28856787</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Animals ; Blastocyst ; Blastocysts ; Blastomeres ; bovine ; Cattle ; Cell Survival ; Cells, Cultured ; Cryoprotectants ; Cryoprotective Agents ; Cryoprotectors ; Developmental stages ; early stages ; Embryo Culture Techniques ; embryo development ; Embryo Transfer ; Embryonic Development ; Embryos ; Ethylene Glycol ; Experiments ; Female ; Fertilization in Vitro ; In Vitro Oocyte Maturation Techniques ; Propylene ; Propylene Glycol ; separated blastomere ; Separation ; Solutions ; Survival ; Vitrification</subject><ispartof>Animal science journal, 2018-01, Vol.89 (1), p.42-51</ispartof><rights>2017 Japanese Society of Animal Science</rights><rights>2017 Japanese Society of Animal Science.</rights><rights>Copyright © 2018 Japanese Society of Animal Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4430-4f5d83a750e3d32966d97d4d6696cfeb8baf0558fe127e33c2ca93ce127409643</citedby><cites>FETCH-LOGICAL-c4430-4f5d83a750e3d32966d97d4d6696cfeb8baf0558fe127e33c2ca93ce127409643</cites><orcidid>0000-0003-4377-6645 ; 0000-0001-9827-5990</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fasj.12890$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fasj.12890$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28856787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juanpanich, Theesit</creatorcontrib><creatorcontrib>Suttirojpattana, Tayita</creatorcontrib><creatorcontrib>Takayama, Mari</creatorcontrib><creatorcontrib>Liang, Yuanyuan</creatorcontrib><creatorcontrib>Dochi, Osamu</creatorcontrib><creatorcontrib>Parnpai, Rangsun</creatorcontrib><creatorcontrib>Imai, Kei</creatorcontrib><title>Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG. In experiment 2, intact two‐cell and eight‐cell stage embryos were vitrified in the same solutions used in experiment 1. The EG + PG produced more dead embryos than the EG (P < 0.05). In the EG, the rate of blastocyst formation was similar for the vitrified two‐ and eight‐cell embryos and the non‐vitrified ywo‐cell embryos. In experiment 3, separated blastomeres of two‐ and eight‐cell embryos were vitrified in EG. There was no difference in the rate of blastocyst formation and total number of cells between the two vitrified groups. In summary, at the blastocyst stage, EG + PG was superior, based on both survival rates and cell numbers; however, at the 2–8 cell stage, the use of EG alone was better than the other groups.</description><subject>Animals</subject><subject>Blastocyst</subject><subject>Blastocysts</subject><subject>Blastomeres</subject><subject>bovine</subject><subject>Cattle</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Cryoprotectants</subject><subject>Cryoprotective Agents</subject><subject>Cryoprotectors</subject><subject>Developmental stages</subject><subject>early stages</subject><subject>Embryo Culture Techniques</subject><subject>embryo development</subject><subject>Embryo Transfer</subject><subject>Embryonic Development</subject><subject>Embryos</subject><subject>Ethylene Glycol</subject><subject>Experiments</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>In Vitro Oocyte Maturation Techniques</subject><subject>Propylene</subject><subject>Propylene Glycol</subject><subject>separated blastomere</subject><subject>Separation</subject><subject>Solutions</subject><subject>Survival</subject><subject>Vitrification</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9KHTEUh0OxVKtd-AIl4KZdjObfzCRLkWorQhfW9ZBJTiSXmck0yUy5L9Nnba7XFhGazUnO-c5H4IfQKSXntJwLnTbnlElF3qAj2gpSEcXUQblzISquBD1E71PaEEJbRep36JBJWTetbI_Q7_slrn7VA9aTxRZWGMI8wpRLx4RxhgyTARwc7sPqJ8Aw9nEbEtYZW-8cxMK-2ktZP0J6EiaYddQZLO4HnXIYC18mLkPEq8_RO2909mHCfnrhS2FYdt10gt46PST48FyP0cP1lx9XX6u77zffri7vKiMEJ5VwtZVctzUBbjlTTWNVa4VtGtUYB73stSN1LR1Q1gLnhhmtuNm9BFGN4Mfo0947x_BzgZS70ScDw6AnCEvqqOKCSaJEW9CzV-gmLHEqvyuUbGrFhGKF-rynTAwpRXDdHP2o47ajpNuF1pXQuqfQCvvx2bj0I9h_5N-UCnCxB375Abb_N3WX97d75R-h4qTR</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Juanpanich, Theesit</creator><creator>Suttirojpattana, Tayita</creator><creator>Takayama, Mari</creator><creator>Liang, Yuanyuan</creator><creator>Dochi, Osamu</creator><creator>Parnpai, Rangsun</creator><creator>Imai, Kei</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4377-6645</orcidid><orcidid>https://orcid.org/0000-0001-9827-5990</orcidid></search><sort><creationdate>201801</creationdate><title>Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions</title><author>Juanpanich, Theesit ; Suttirojpattana, Tayita ; Takayama, Mari ; Liang, Yuanyuan ; Dochi, Osamu ; Parnpai, Rangsun ; Imai, Kei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-4f5d83a750e3d32966d97d4d6696cfeb8baf0558fe127e33c2ca93ce127409643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Blastocyst</topic><topic>Blastocysts</topic><topic>Blastomeres</topic><topic>bovine</topic><topic>Cattle</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Cryoprotectants</topic><topic>Cryoprotective Agents</topic><topic>Cryoprotectors</topic><topic>Developmental stages</topic><topic>early stages</topic><topic>Embryo Culture Techniques</topic><topic>embryo development</topic><topic>Embryo Transfer</topic><topic>Embryonic Development</topic><topic>Embryos</topic><topic>Ethylene Glycol</topic><topic>Experiments</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>In Vitro Oocyte Maturation Techniques</topic><topic>Propylene</topic><topic>Propylene Glycol</topic><topic>separated blastomere</topic><topic>Separation</topic><topic>Solutions</topic><topic>Survival</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juanpanich, Theesit</creatorcontrib><creatorcontrib>Suttirojpattana, Tayita</creatorcontrib><creatorcontrib>Takayama, Mari</creatorcontrib><creatorcontrib>Liang, Yuanyuan</creatorcontrib><creatorcontrib>Dochi, Osamu</creatorcontrib><creatorcontrib>Parnpai, Rangsun</creatorcontrib><creatorcontrib>Imai, Kei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juanpanich, Theesit</au><au>Suttirojpattana, Tayita</au><au>Takayama, Mari</au><au>Liang, Yuanyuan</au><au>Dochi, Osamu</au><au>Parnpai, Rangsun</au><au>Imai, Kei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2018-01</date><risdate>2018</risdate><volume>89</volume><issue>1</issue><spage>42</spage><epage>51</epage><pages>42-51</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG. In experiment 2, intact two‐cell and eight‐cell stage embryos were vitrified in the same solutions used in experiment 1. The EG + PG produced more dead embryos than the EG (P < 0.05). In the EG, the rate of blastocyst formation was similar for the vitrified two‐ and eight‐cell embryos and the non‐vitrified ywo‐cell embryos. In experiment 3, separated blastomeres of two‐ and eight‐cell embryos were vitrified in EG. There was no difference in the rate of blastocyst formation and total number of cells between the two vitrified groups. In summary, at the blastocyst stage, EG + PG was superior, based on both survival rates and cell numbers; however, at the 2–8 cell stage, the use of EG alone was better than the other groups.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>28856787</pmid><doi>10.1111/asj.12890</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4377-6645</orcidid><orcidid>https://orcid.org/0000-0001-9827-5990</orcidid></addata></record> |
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| identifier | ISSN: 1344-3941 |
| ispartof | Animal science journal, 2018-01, Vol.89 (1), p.42-51 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library; Alma/SFX Local Collection |
| subjects | Animals Blastocyst Blastocysts Blastomeres bovine Cattle Cell Survival Cells, Cultured Cryoprotectants Cryoprotective Agents Cryoprotectors Developmental stages early stages Embryo Culture Techniques embryo development Embryo Transfer Embryonic Development Embryos Ethylene Glycol Experiments Female Fertilization in Vitro In Vitro Oocyte Maturation Techniques Propylene Propylene Glycol separated blastomere Separation Solutions Survival Vitrification |
| title | Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions |
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