Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum

Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in phy...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ACS sensors 2017-09, Vol.2 (9), p.1278-1286
Hauptverfasser: Gutiérrez-Sanz, Óscar, Andoy, Nesha M, Filipiak, Marcin S, Haustein, Natalie, Tarasov, Alexey
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1286
container_issue 9
container_start_page 1278
container_title ACS sensors
container_volume 2
creator Gutiérrez-Sanz, Óscar
Andoy, Nesha M
Filipiak, Marcin S
Haustein, Natalie
Tarasov, Alexey
description Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in physiological samples (
doi_str_mv 10.1021/acssensors.7b00187
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1933939445</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1933939445</sourcerecordid><originalsourceid>FETCH-LOGICAL-a342t-6759d68b46033ffe6ab6ac610dba7637dd27a5d1b490488285cf95fbc2d314ea3</originalsourceid><addsrcrecordid>eNp9kDtPwzAURi0EolXpH2BAGRma4lcce4RCAakICYoYIzu-EakSu9jJwL8nqOUxMfkO53ySD0KnBM8JpuRClzGCiz7EeW4wJjI_QGPKcpUyofjhn3uEpjFu8MBkgmYSH6MRlTJjVLIxeriuA5TdLFlpA026DACzRDubPOltbZN10C7WsfMhvdIRbHLftr3zFrpBqr1Lape8vvkGkmcIfXuCjirdRJju3wl6Wd6sF3fp6vH2fnG5SjXjtEtFnikrpOECM1ZVILQRuhQEW6NzwXJraa4zSwxXmEtJZVZWKqtMSS0jHDSboPPd7jb49x5iV7R1LKFptAPfx4IoxhRTnGcDSndoGXyMAapiG-pWh4-C4OKrZPFbstiXHKSz_X5vWrA_yne3AZjvgEEuNr4Pbvjuf4ufGQqA7w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1933939445</pqid></control><display><type>article</type><title>Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum</title><source>ACS Publications</source><creator>Gutiérrez-Sanz, Óscar ; Andoy, Nesha M ; Filipiak, Marcin S ; Haustein, Natalie ; Tarasov, Alexey</creator><creatorcontrib>Gutiérrez-Sanz, Óscar ; Andoy, Nesha M ; Filipiak, Marcin S ; Haustein, Natalie ; Tarasov, Alexey</creatorcontrib><description>Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in physiological samples (&lt;1 nm) has been a major drawback so far, preventing direct measurements in serum. In this work, we demonstrate how tailoring the sensing surface with short specific biological receptors and a polymer polyethylene glycol (PEG) can strongly enhance the sensor response. In addition, the sensor performance can be dramatically improved if the measurements are performed at elevated temperatures (37 °C instead of 21 °C). With this novel approach, highly sensitive and selective detection of a representative immunosensing parameterhuman thyroid-stimulating hormoneis shown over a wide measuring range with subpicomolar detection limits in whole serum. To the best of our knowledge, this is the first demonstration of direct immunodetection in whole serum using transistor-based biosensors, without the need for sample pretreatment, labeling, or washing steps. The presented sensor is low-cost, can be easily integrated into portable diagnostics devices, and offers a competitive performance compared to state-of-the-art central laboratory analyzers.</description><identifier>ISSN: 2379-3694</identifier><identifier>EISSN: 2379-3694</identifier><identifier>DOI: 10.1021/acssensors.7b00187</identifier><identifier>PMID: 28853283</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><ispartof>ACS sensors, 2017-09, Vol.2 (9), p.1278-1286</ispartof><rights>Copyright © 2017 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a342t-6759d68b46033ffe6ab6ac610dba7637dd27a5d1b490488285cf95fbc2d314ea3</citedby><cites>FETCH-LOGICAL-a342t-6759d68b46033ffe6ab6ac610dba7637dd27a5d1b490488285cf95fbc2d314ea3</cites><orcidid>0000-0002-5326-3328 ; 0000-0001-7559-9204 ; 0000-0003-2811-5988 ; 0000-0002-7649-4890</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acssensors.7b00187$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acssensors.7b00187$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28853283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gutiérrez-Sanz, Óscar</creatorcontrib><creatorcontrib>Andoy, Nesha M</creatorcontrib><creatorcontrib>Filipiak, Marcin S</creatorcontrib><creatorcontrib>Haustein, Natalie</creatorcontrib><creatorcontrib>Tarasov, Alexey</creatorcontrib><title>Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum</title><title>ACS sensors</title><addtitle>ACS Sens</addtitle><description>Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in physiological samples (&lt;1 nm) has been a major drawback so far, preventing direct measurements in serum. In this work, we demonstrate how tailoring the sensing surface with short specific biological receptors and a polymer polyethylene glycol (PEG) can strongly enhance the sensor response. In addition, the sensor performance can be dramatically improved if the measurements are performed at elevated temperatures (37 °C instead of 21 °C). With this novel approach, highly sensitive and selective detection of a representative immunosensing parameterhuman thyroid-stimulating hormoneis shown over a wide measuring range with subpicomolar detection limits in whole serum. To the best of our knowledge, this is the first demonstration of direct immunodetection in whole serum using transistor-based biosensors, without the need for sample pretreatment, labeling, or washing steps. The presented sensor is low-cost, can be easily integrated into portable diagnostics devices, and offers a competitive performance compared to state-of-the-art central laboratory analyzers.</description><issn>2379-3694</issn><issn>2379-3694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPwzAURi0EolXpH2BAGRma4lcce4RCAakICYoYIzu-EakSu9jJwL8nqOUxMfkO53ySD0KnBM8JpuRClzGCiz7EeW4wJjI_QGPKcpUyofjhn3uEpjFu8MBkgmYSH6MRlTJjVLIxeriuA5TdLFlpA026DACzRDubPOltbZN10C7WsfMhvdIRbHLftr3zFrpBqr1Lape8vvkGkmcIfXuCjirdRJju3wl6Wd6sF3fp6vH2fnG5SjXjtEtFnikrpOECM1ZVILQRuhQEW6NzwXJraa4zSwxXmEtJZVZWKqtMSS0jHDSboPPd7jb49x5iV7R1LKFptAPfx4IoxhRTnGcDSndoGXyMAapiG-pWh4-C4OKrZPFbstiXHKSz_X5vWrA_yne3AZjvgEEuNr4Pbvjuf4ufGQqA7w</recordid><startdate>20170922</startdate><enddate>20170922</enddate><creator>Gutiérrez-Sanz, Óscar</creator><creator>Andoy, Nesha M</creator><creator>Filipiak, Marcin S</creator><creator>Haustein, Natalie</creator><creator>Tarasov, Alexey</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5326-3328</orcidid><orcidid>https://orcid.org/0000-0001-7559-9204</orcidid><orcidid>https://orcid.org/0000-0003-2811-5988</orcidid><orcidid>https://orcid.org/0000-0002-7649-4890</orcidid></search><sort><creationdate>20170922</creationdate><title>Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum</title><author>Gutiérrez-Sanz, Óscar ; Andoy, Nesha M ; Filipiak, Marcin S ; Haustein, Natalie ; Tarasov, Alexey</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a342t-6759d68b46033ffe6ab6ac610dba7637dd27a5d1b490488285cf95fbc2d314ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez-Sanz, Óscar</creatorcontrib><creatorcontrib>Andoy, Nesha M</creatorcontrib><creatorcontrib>Filipiak, Marcin S</creatorcontrib><creatorcontrib>Haustein, Natalie</creatorcontrib><creatorcontrib>Tarasov, Alexey</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS sensors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutiérrez-Sanz, Óscar</au><au>Andoy, Nesha M</au><au>Filipiak, Marcin S</au><au>Haustein, Natalie</au><au>Tarasov, Alexey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum</atitle><jtitle>ACS sensors</jtitle><addtitle>ACS Sens</addtitle><date>2017-09-22</date><risdate>2017</risdate><volume>2</volume><issue>9</issue><spage>1278</spage><epage>1286</epage><pages>1278-1286</pages><issn>2379-3694</issn><eissn>2379-3694</eissn><abstract>Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in physiological samples (&lt;1 nm) has been a major drawback so far, preventing direct measurements in serum. In this work, we demonstrate how tailoring the sensing surface with short specific biological receptors and a polymer polyethylene glycol (PEG) can strongly enhance the sensor response. In addition, the sensor performance can be dramatically improved if the measurements are performed at elevated temperatures (37 °C instead of 21 °C). With this novel approach, highly sensitive and selective detection of a representative immunosensing parameterhuman thyroid-stimulating hormoneis shown over a wide measuring range with subpicomolar detection limits in whole serum. To the best of our knowledge, this is the first demonstration of direct immunodetection in whole serum using transistor-based biosensors, without the need for sample pretreatment, labeling, or washing steps. The presented sensor is low-cost, can be easily integrated into portable diagnostics devices, and offers a competitive performance compared to state-of-the-art central laboratory analyzers.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>28853283</pmid><doi>10.1021/acssensors.7b00187</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5326-3328</orcidid><orcidid>https://orcid.org/0000-0001-7559-9204</orcidid><orcidid>https://orcid.org/0000-0003-2811-5988</orcidid><orcidid>https://orcid.org/0000-0002-7649-4890</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 2379-3694
ispartof ACS sensors, 2017-09, Vol.2 (9), p.1278-1286
issn 2379-3694
2379-3694
language eng
recordid cdi_proquest_miscellaneous_1933939445
source ACS Publications
title Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T07%3A37%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct,%20Label-Free,%20and%20Rapid%20Transistor-Based%20Immunodetection%20in%20Whole%20Serum&rft.jtitle=ACS%20sensors&rft.au=Gutie%CC%81rrez-Sanz,%20O%CC%81scar&rft.date=2017-09-22&rft.volume=2&rft.issue=9&rft.spage=1278&rft.epage=1286&rft.pages=1278-1286&rft.issn=2379-3694&rft.eissn=2379-3694&rft_id=info:doi/10.1021/acssensors.7b00187&rft_dat=%3Cproquest_cross%3E1933939445%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1933939445&rft_id=info:pmid/28853283&rfr_iscdi=true