Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR
The aim of our work was to detect Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of L. monocytogenes, using specific primers to amplify a 149 bp fragment from the metalloprote...
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| Veröffentlicht in: | Food control 2009-04, Vol.20 (4), p.430-432 |
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| creator | Vanegas, Maria Consuelo Vásquez, Elizabeth Martinez, Aida Juliana Rueda, Adriana M. |
| description | The aim of our work was to detect
Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of
L. monocytogenes, using specific primers to amplify a 149
bp fragment from the metalloprotease gene (
mpl). In this study on a total number of 81 samples, 21 gave positive results for
L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods. |
| doi_str_mv | 10.1016/j.foodcont.2008.07.007 |
| format | Article |
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Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of
L. monocytogenes, using specific primers to amplify a 149
bp fragment from the metalloprotease gene (
mpl). In this study on a total number of 81 samples, 21 gave positive results for
L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods.</description><identifier>ISSN: 0956-7135</identifier><identifier>EISSN: 1873-7129</identifier><identifier>DOI: 10.1016/j.foodcont.2008.07.007</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>bacterial contamination ; food contamination ; food pathogens ; Listeria monocytogenes ; microbial detection ; milk analysis ; milk composition ; polymerase chain reaction ; raw milk ; Raw whole milk ; Real-time PCR ; real-time polymerase chain reaction ; whole milk</subject><ispartof>Food control, 2009-04, Vol.20 (4), p.430-432</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-7f1a757b72a281b75e0e7ea67d6cfc0bab2c77e03016b2305e60a3c18efdd08b3</citedby><cites>FETCH-LOGICAL-c367t-7f1a757b72a281b75e0e7ea67d6cfc0bab2c77e03016b2305e60a3c18efdd08b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956713508001795$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Vanegas, Maria Consuelo</creatorcontrib><creatorcontrib>Vásquez, Elizabeth</creatorcontrib><creatorcontrib>Martinez, Aida Juliana</creatorcontrib><creatorcontrib>Rueda, Adriana M.</creatorcontrib><title>Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR</title><title>Food control</title><description>The aim of our work was to detect
Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of
L. monocytogenes, using specific primers to amplify a 149
bp fragment from the metalloprotease gene (
mpl). In this study on a total number of 81 samples, 21 gave positive results for
L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods.</description><subject>bacterial contamination</subject><subject>food contamination</subject><subject>food pathogens</subject><subject>Listeria monocytogenes</subject><subject>microbial detection</subject><subject>milk analysis</subject><subject>milk composition</subject><subject>polymerase chain reaction</subject><subject>raw milk</subject><subject>Raw whole milk</subject><subject>Real-time PCR</subject><subject>real-time polymerase chain reaction</subject><subject>whole milk</subject><issn>0956-7135</issn><issn>1873-7129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkE-P0zAQxS3ESpRdvgL4xC1hHCue9AYqf6VKoIU9W44z3rokmWKnu-q3x6Vw5jRzeO_NvJ8QLxXUCpR5s68D8-B5XuoGoKsBawB8IlaqQ12hatZPxQrWrSm7bp-J5znvARSCgpXYvaeF_BJ5lhzkNuaFUnRy4pn9aeF7minLOMvkHuXjjkeSUxx_ysBJ7o6Tm2W5m4_T4U9C0W145KkvCf1JJnJjtcSJ5LfN7Y24Cm7M9OLvvBZ3Hz_82Hyutl8_fdm821ZeG1wqDMphiz02rulUjy0BITmDg_HBQ-_6xiMS6NK8bzS0ZMBprzoKwwBdr6_F60vuIfGvI-XFTjF7Gkc3Ex-zVWutmw5MEZqL0CfOOVGwhxQnl05WgT2DtXv7D6w9g7WAtoAtxlcXY3Bs3X2K2d59b0CVl1qjOzxHv70oqBR9iJRs9pFmT0NMBbYdOP7vyG9VCY_6</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Vanegas, Maria Consuelo</creator><creator>Vásquez, Elizabeth</creator><creator>Martinez, Aida Juliana</creator><creator>Rueda, Adriana M.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090401</creationdate><title>Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR</title><author>Vanegas, Maria Consuelo ; Vásquez, Elizabeth ; Martinez, Aida Juliana ; Rueda, Adriana M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-7f1a757b72a281b75e0e7ea67d6cfc0bab2c77e03016b2305e60a3c18efdd08b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>bacterial contamination</topic><topic>food contamination</topic><topic>food pathogens</topic><topic>Listeria monocytogenes</topic><topic>microbial detection</topic><topic>milk analysis</topic><topic>milk composition</topic><topic>polymerase chain reaction</topic><topic>raw milk</topic><topic>Raw whole milk</topic><topic>Real-time PCR</topic><topic>real-time polymerase chain reaction</topic><topic>whole milk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vanegas, Maria Consuelo</creatorcontrib><creatorcontrib>Vásquez, Elizabeth</creatorcontrib><creatorcontrib>Martinez, Aida Juliana</creatorcontrib><creatorcontrib>Rueda, Adriana M.</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vanegas, Maria Consuelo</au><au>Vásquez, Elizabeth</au><au>Martinez, Aida Juliana</au><au>Rueda, Adriana M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR</atitle><jtitle>Food control</jtitle><date>2009-04-01</date><risdate>2009</risdate><volume>20</volume><issue>4</issue><spage>430</spage><epage>432</epage><pages>430-432</pages><issn>0956-7135</issn><eissn>1873-7129</eissn><abstract>The aim of our work was to detect
Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of
L. monocytogenes, using specific primers to amplify a 149
bp fragment from the metalloprotease gene (
mpl). In this study on a total number of 81 samples, 21 gave positive results for
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| ispartof | Food control, 2009-04, Vol.20 (4), p.430-432 |
| issn | 0956-7135 1873-7129 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | bacterial contamination food contamination food pathogens Listeria monocytogenes microbial detection milk analysis milk composition polymerase chain reaction raw milk Raw whole milk Real-time PCR real-time polymerase chain reaction whole milk |
| title | Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR |
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