An Efficient Method for Generation of Knockout Human Embryonic Stem Cells Using CRISPR/Cas9 System

Human embryonic stem cells (hESCs) represent a promising tool to study functions of genes during development, to model diseases, and to even develop therapies when combined with gene editing techniques such as CRISPR/CRISPR-associated protein-9 nuclease (Cas9) system. However, the process of disrupt...

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Veröffentlicht in:	Stem cells and development 2017-11, Vol.26 (21), p.1521-1527
Hauptverfasser:	Bohaciakova, Dasa, Renzova, Tereza, Fedorova, Veronika, Barak, Martin, Kunova Bosakova, Michaela, Hampl, Ales, Cajanek, Lukas
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Line Cells, Cultured CRISPR-Cas Systems Down-Regulation Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Gene Knockout Techniques - methods Humans Mice MicroRNAs - genetics Technical Note Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism
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