Comparison of human umbilical cord blood processing with or without hydroxyethyl starch
BACKGROUND Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell rec...
Gespeichert in:
| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2017-11, Vol.57 (11), p.2758-2766 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2766 |
|---|---|
| container_issue | 11 |
| container_start_page | 2758 |
| container_title | Transfusion (Philadelphia, Pa.) |
| container_volume | 57 |
| creator | Souri, Milad Nikougoftar Zarif, Mahin Rasouli, Mahboobeh Golzadeh, Khadijeh Nakhlestani Hagh, Mozhdeh Ezzati, Nasim Atarodi, Kamran |
| description | BACKGROUND
Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell recovery and viability of UCB processed using the Sepax automated system in the presence and absence of HES.
STUDY DESIGN AND METHODS
Thirty UCB bags with a total nucleated cell (TNC) count of more than 2.5 × 109 were divided in two bags with equal volume. HES solution was added to one bag and another was intact. Both bags were processed with the Sepax. To determine cell recovery, viability, and potential of colony‐forming cells (CFCs), preprocessing, postprocessing, and thawing samples were analyzed.
RESULTS
The mean TNC recovery after processing and after thaw was significantly better with the HES method (p |
| doi_str_mv | 10.1111/trf.14290 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1932162744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1932162744</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-6d0c4fe8bf7e85d6e53086524512640910e0b188f078b9449e25770aa64f0be13</originalsourceid><addsrcrecordid>eNp10EFLwzAUB_AgipvTg19AAl700O0lTdv0KMOpMBBk4rGkaWo70mYmLbPf3rhND4Lv8uDx48_jj9AlgSnxM-tsOSWMpnCExiQKk4CmaXSMxgCMBISEdITOnFsDgDfkFI0o52Ecchijt7lpNsLWzrTYlLjqG9HivslrXUuhsTS2wLk2psAba6Ryrm7f8bbuKmzsbpu-w9VQWPM5qK4aNHadsLI6Ryel0E5dHPYEvS7uV_PHYPn88DS_WwYyjEII4gIkKxXPy0TxqIiVP_I4oiwiNGaQElCQE85LSHieMpYqGiUJCBGzEnJFwgm62ef69z565bqsqZ1UWotWmd5lJA0piWnCmKfXf-ja9Lb133kVA0up79Cr272S1jhnVZltbN0IO2QEsu-2M992tmvb26tDYp83qviVP_V6MNuDba3V8H9StnpZ7CO_AEVsiEs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1960492142</pqid></control><display><type>article</type><title>Comparison of human umbilical cord blood processing with or without hydroxyethyl starch</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Souri, Milad ; Nikougoftar Zarif, Mahin ; Rasouli, Mahboobeh ; Golzadeh, Khadijeh ; Nakhlestani Hagh, Mozhdeh ; Ezzati, Nasim ; Atarodi, Kamran</creator><creatorcontrib>Souri, Milad ; Nikougoftar Zarif, Mahin ; Rasouli, Mahboobeh ; Golzadeh, Khadijeh ; Nakhlestani Hagh, Mozhdeh ; Ezzati, Nasim ; Atarodi, Kamran</creatorcontrib><description>BACKGROUND
Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell recovery and viability of UCB processed using the Sepax automated system in the presence and absence of HES.
STUDY DESIGN AND METHODS
Thirty UCB bags with a total nucleated cell (TNC) count of more than 2.5 × 109 were divided in two bags with equal volume. HES solution was added to one bag and another was intact. Both bags were processed with the Sepax. To determine cell recovery, viability, and potential of colony‐forming cells (CFCs), preprocessing, postprocessing, and thawing samples were analyzed.
RESULTS
The mean TNC recovery after processing and after thaw was significantly better with the HES method (p < 0.01 for the postprocessing step and p < 0.05 for the postthaw step). There were no significant differences to mononucleated cells (MNCs) and CD34+ cell recovery between the two methods after processing and after thaw. TNC and MNC viability was significantly higher without HES after processing and after thaw (p < 0.01). The results of the CFC assay were similar for both methods after processing and after thaw.
CONCLUSION
These results showed that processing of UCB using the Sepax system with the without‐HES protocol due to the lower manipulation of samples could be used as an eligible protocol to reduce the volume of UCB.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.14290</identifier><identifier>PMID: 28836380</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antigens, CD34 - analysis ; Blood ; Blood Banking - methods ; Blood Banks - standards ; CD34 antigen ; Cell Count ; Cell Survival ; Chlorofluorocarbons ; Colony-forming cells ; Cord blood ; Cryopreservation ; Fetal Blood - cytology ; Humans ; Hydroxyethyl starch ; Hydroxyethyl Starch Derivatives - pharmacology ; Infant, Newborn ; Preprocessing ; Recovery ; Starch ; Stem Cells - cytology ; Thawing ; Umbilical cord ; Viability</subject><ispartof>Transfusion (Philadelphia, Pa.), 2017-11, Vol.57 (11), p.2758-2766</ispartof><rights>2017 AABB</rights><rights>2017 AABB.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-6d0c4fe8bf7e85d6e53086524512640910e0b188f078b9449e25770aa64f0be13</citedby><cites>FETCH-LOGICAL-c3530-6d0c4fe8bf7e85d6e53086524512640910e0b188f078b9449e25770aa64f0be13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.14290$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.14290$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28836380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Souri, Milad</creatorcontrib><creatorcontrib>Nikougoftar Zarif, Mahin</creatorcontrib><creatorcontrib>Rasouli, Mahboobeh</creatorcontrib><creatorcontrib>Golzadeh, Khadijeh</creatorcontrib><creatorcontrib>Nakhlestani Hagh, Mozhdeh</creatorcontrib><creatorcontrib>Ezzati, Nasim</creatorcontrib><creatorcontrib>Atarodi, Kamran</creatorcontrib><title>Comparison of human umbilical cord blood processing with or without hydroxyethyl starch</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND
Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell recovery and viability of UCB processed using the Sepax automated system in the presence and absence of HES.
STUDY DESIGN AND METHODS
Thirty UCB bags with a total nucleated cell (TNC) count of more than 2.5 × 109 were divided in two bags with equal volume. HES solution was added to one bag and another was intact. Both bags were processed with the Sepax. To determine cell recovery, viability, and potential of colony‐forming cells (CFCs), preprocessing, postprocessing, and thawing samples were analyzed.
RESULTS
The mean TNC recovery after processing and after thaw was significantly better with the HES method (p < 0.01 for the postprocessing step and p < 0.05 for the postthaw step). There were no significant differences to mononucleated cells (MNCs) and CD34+ cell recovery between the two methods after processing and after thaw. TNC and MNC viability was significantly higher without HES after processing and after thaw (p < 0.01). The results of the CFC assay were similar for both methods after processing and after thaw.
CONCLUSION
These results showed that processing of UCB using the Sepax system with the without‐HES protocol due to the lower manipulation of samples could be used as an eligible protocol to reduce the volume of UCB.</description><subject>Antigens, CD34 - analysis</subject><subject>Blood</subject><subject>Blood Banking - methods</subject><subject>Blood Banks - standards</subject><subject>CD34 antigen</subject><subject>Cell Count</subject><subject>Cell Survival</subject><subject>Chlorofluorocarbons</subject><subject>Colony-forming cells</subject><subject>Cord blood</subject><subject>Cryopreservation</subject><subject>Fetal Blood - cytology</subject><subject>Humans</subject><subject>Hydroxyethyl starch</subject><subject>Hydroxyethyl Starch Derivatives - pharmacology</subject><subject>Infant, Newborn</subject><subject>Preprocessing</subject><subject>Recovery</subject><subject>Starch</subject><subject>Stem Cells - cytology</subject><subject>Thawing</subject><subject>Umbilical cord</subject><subject>Viability</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10EFLwzAUB_AgipvTg19AAl700O0lTdv0KMOpMBBk4rGkaWo70mYmLbPf3rhND4Lv8uDx48_jj9AlgSnxM-tsOSWMpnCExiQKk4CmaXSMxgCMBISEdITOnFsDgDfkFI0o52Ecchijt7lpNsLWzrTYlLjqG9HivslrXUuhsTS2wLk2psAba6Ryrm7f8bbuKmzsbpu-w9VQWPM5qK4aNHadsLI6Ryel0E5dHPYEvS7uV_PHYPn88DS_WwYyjEII4gIkKxXPy0TxqIiVP_I4oiwiNGaQElCQE85LSHieMpYqGiUJCBGzEnJFwgm62ef69z565bqsqZ1UWotWmd5lJA0piWnCmKfXf-ja9Lb133kVA0up79Cr272S1jhnVZltbN0IO2QEsu-2M992tmvb26tDYp83qviVP_V6MNuDba3V8H9StnpZ7CO_AEVsiEs</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Souri, Milad</creator><creator>Nikougoftar Zarif, Mahin</creator><creator>Rasouli, Mahboobeh</creator><creator>Golzadeh, Khadijeh</creator><creator>Nakhlestani Hagh, Mozhdeh</creator><creator>Ezzati, Nasim</creator><creator>Atarodi, Kamran</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201711</creationdate><title>Comparison of human umbilical cord blood processing with or without hydroxyethyl starch</title><author>Souri, Milad ; Nikougoftar Zarif, Mahin ; Rasouli, Mahboobeh ; Golzadeh, Khadijeh ; Nakhlestani Hagh, Mozhdeh ; Ezzati, Nasim ; Atarodi, Kamran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-6d0c4fe8bf7e85d6e53086524512640910e0b188f078b9449e25770aa64f0be13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antigens, CD34 - analysis</topic><topic>Blood</topic><topic>Blood Banking - methods</topic><topic>Blood Banks - standards</topic><topic>CD34 antigen</topic><topic>Cell Count</topic><topic>Cell Survival</topic><topic>Chlorofluorocarbons</topic><topic>Colony-forming cells</topic><topic>Cord blood</topic><topic>Cryopreservation</topic><topic>Fetal Blood - cytology</topic><topic>Humans</topic><topic>Hydroxyethyl starch</topic><topic>Hydroxyethyl Starch Derivatives - pharmacology</topic><topic>Infant, Newborn</topic><topic>Preprocessing</topic><topic>Recovery</topic><topic>Starch</topic><topic>Stem Cells - cytology</topic><topic>Thawing</topic><topic>Umbilical cord</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Souri, Milad</creatorcontrib><creatorcontrib>Nikougoftar Zarif, Mahin</creatorcontrib><creatorcontrib>Rasouli, Mahboobeh</creatorcontrib><creatorcontrib>Golzadeh, Khadijeh</creatorcontrib><creatorcontrib>Nakhlestani Hagh, Mozhdeh</creatorcontrib><creatorcontrib>Ezzati, Nasim</creatorcontrib><creatorcontrib>Atarodi, Kamran</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Souri, Milad</au><au>Nikougoftar Zarif, Mahin</au><au>Rasouli, Mahboobeh</au><au>Golzadeh, Khadijeh</au><au>Nakhlestani Hagh, Mozhdeh</au><au>Ezzati, Nasim</au><au>Atarodi, Kamran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of human umbilical cord blood processing with or without hydroxyethyl starch</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2017-11</date><risdate>2017</risdate><volume>57</volume><issue>11</issue><spage>2758</spage><epage>2766</epage><pages>2758-2766</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><abstract>BACKGROUND
Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell recovery and viability of UCB processed using the Sepax automated system in the presence and absence of HES.
STUDY DESIGN AND METHODS
Thirty UCB bags with a total nucleated cell (TNC) count of more than 2.5 × 109 were divided in two bags with equal volume. HES solution was added to one bag and another was intact. Both bags were processed with the Sepax. To determine cell recovery, viability, and potential of colony‐forming cells (CFCs), preprocessing, postprocessing, and thawing samples were analyzed.
RESULTS
The mean TNC recovery after processing and after thaw was significantly better with the HES method (p < 0.01 for the postprocessing step and p < 0.05 for the postthaw step). There were no significant differences to mononucleated cells (MNCs) and CD34+ cell recovery between the two methods after processing and after thaw. TNC and MNC viability was significantly higher without HES after processing and after thaw (p < 0.01). The results of the CFC assay were similar for both methods after processing and after thaw.
CONCLUSION
These results showed that processing of UCB using the Sepax system with the without‐HES protocol due to the lower manipulation of samples could be used as an eligible protocol to reduce the volume of UCB.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28836380</pmid><doi>10.1111/trf.14290</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-1132 |
| ispartof | Transfusion (Philadelphia, Pa.), 2017-11, Vol.57 (11), p.2758-2766 |
| issn | 0041-1132 1537-2995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1932162744 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antigens, CD34 - analysis Blood Blood Banking - methods Blood Banks - standards CD34 antigen Cell Count Cell Survival Chlorofluorocarbons Colony-forming cells Cord blood Cryopreservation Fetal Blood - cytology Humans Hydroxyethyl starch Hydroxyethyl Starch Derivatives - pharmacology Infant, Newborn Preprocessing Recovery Starch Stem Cells - cytology Thawing Umbilical cord Viability |
| title | Comparison of human umbilical cord blood processing with or without hydroxyethyl starch |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T13%3A22%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20human%20umbilical%20cord%20blood%20processing%20with%20or%20without%20hydroxyethyl%20starch&rft.jtitle=Transfusion%20(Philadelphia,%20Pa.)&rft.au=Souri,%20Milad&rft.date=2017-11&rft.volume=57&rft.issue=11&rft.spage=2758&rft.epage=2766&rft.pages=2758-2766&rft.issn=0041-1132&rft.eissn=1537-2995&rft_id=info:doi/10.1111/trf.14290&rft_dat=%3Cproquest_cross%3E1932162744%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1960492142&rft_id=info:pmid/28836380&rfr_iscdi=true |