E prostanoid 2 (EP2)-EP4-mediated suppression of antigen-specific human T-cell responses by prostaglandin E sub(2)

Prostaglandin E sub(2) (PGE sub(2)) is a lipid mediator that displays important immunomodulatory properties, such as polarization of cytokine production by T cells. Recent investigations have revealed that the effect of PGE sub(2) on cytokine production is greatly influenced by external stimuli; how...

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Veröffentlicht in:Immunology 2006-07, Vol.118 (3), p.343-352
Hauptverfasser: Okano, Mitsuhiro, Sugata, Yuji, Fujiwara, Tazuko, Matsumoto, Rie, Nishibori, Masahiro, Shimizu, Kenji, Maeda, Megumi, Kimura, Yoshinobu, Kariya, Shin, Hattori, Hisashi, Yokoyama, Minehiko, Kino, Kosuke, Nishizaki, Kazunori
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container_issue 3
container_start_page 343
container_title Immunology
container_volume 118
creator Okano, Mitsuhiro
Sugata, Yuji
Fujiwara, Tazuko
Matsumoto, Rie
Nishibori, Masahiro
Shimizu, Kenji
Maeda, Megumi
Kimura, Yoshinobu
Kariya, Shin
Hattori, Hisashi
Yokoyama, Minehiko
Kino, Kosuke
Nishizaki, Kazunori
description Prostaglandin E sub(2) (PGE sub(2)) is a lipid mediator that displays important immunomodulatory properties, such as polarization of cytokine production by T cells. Recent investigations have revealed that the effect of PGE sub(2) on cytokine production is greatly influenced by external stimuli; however, it is unclear whether PGE sub(2) plays a significant role in major histocompatibility complex-mediated antigen-specific T-cell responses via binding to one of four subtypes of E prostanoid (EP) receptor alone or in combination. In the present study, we sought to determine the effect of PGE sub(2) on antigen-specific CD4 super(+) T-cell responses in humans, especially in terms of receptor specificity. We used purified protein derivative (PPD) and Cry j 1 as T helper type 1 (Th1) and Th2-inducing antigens, respectively. We generated several different Cry j 1- and PPD-specific T-cell lines (TCLs). PGE sub(2) significantly and dose-dependently inhibited the proliferation and subsequent production of interleukin-4 by Cry j 1-specific TCLs and of interferon- gamma by PPD-specific TCLs upon antigen stimulation. Administration of EP2 receptor agonist and EP4 receptor agonist suppressed these responses in an adenylate cyclase-dependent manner, while EP1 and EP3 receptor agonists did not. Messenger RNA for EP2, EP3 and EP4, but not EP1, receptors were detected in Cry j 1- and PPD-specific TCLs, and no differences in EP receptor expression were observed between them. Furthermore, PGE sub(2) and EP2 receptor agonist significantly inhibited interleukin-5 and interferon- gamma production by peripheral blood mononuclear cells in response to Cry j 1 and PPD stimulation, respectively. These results suggest that PGE sub(2) suppresses both Th1- and Th2-polarized antigen-specific human T-cell responses via a cAMP-dependent EP2-EP4-mediated pathway.
doi_str_mv 10.1111/j.1365-2567.2006.02376.x
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Administration of EP2 receptor agonist and EP4 receptor agonist suppressed these responses in an adenylate cyclase-dependent manner, while EP1 and EP3 receptor agonists did not. Messenger RNA for EP2, EP3 and EP4, but not EP1, receptors were detected in Cry j 1- and PPD-specific TCLs, and no differences in EP receptor expression were observed between them. Furthermore, PGE sub(2) and EP2 receptor agonist significantly inhibited interleukin-5 and interferon- gamma production by peripheral blood mononuclear cells in response to Cry j 1 and PPD stimulation, respectively. 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title E prostanoid 2 (EP2)-EP4-mediated suppression of antigen-specific human T-cell responses by prostaglandin E sub(2)
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