Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss
Aim Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women wi...
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| Veröffentlicht in: | The journal of obstetrics and gynaecology research 2017-11, Vol.43 (11), p.1678-1686 |
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| container_title | The journal of obstetrics and gynaecology research |
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| creator | Fukui, Atsushi Funamizu, Ayano Fukuhara, Rie Shibahara, Hiroaki |
| description | Aim
Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women with recurrent pregnancy loss (RPL). We also investigated the expression of NCR in peripheral blood NK cells in pregnant women with and without a history of RPL.
Methods
The expression of NCR (NKp46, NKp44, and NKp30) in NK cells (CD56dim and CD56bright) in the uterine endometrium was analyzed using 3‐color flow cytometry. Cytokine (tumor necrosis factor‐α and interferon‐γ) production was also analyzed. NK cells from the mid‐secretory endometrium of 28 women with RPL, 34 women with implantation failure, and 74 controls were collected and mechanically dispersed using a tissue grinder. The expression of NCR in peripheral blood NK cells from pregnant women with (n = 17) and without (n = 91) a history of RPL was analyzed.
Results
The percentages of NKp46+ NK cells were significantly lower in both women with RPL and pregnant women with a history of RPL. The percentages of tumor necrosis factor‐α‐ and/or interferon‐γ‐producing uterine endometrial NK cells were significantly lower in women with RPL compared with controls.
Conclusion
The changes in NCR expression and cytokine production, especially decreased NKp46 expression in endometrial NK cells, suggests the presence of abnormal NK cell regulation in women with reproductive failures. |
| doi_str_mv | 10.1111/jog.13448 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1930487287</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1930487287</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4128-732021cadd334409e19fb854c20d1027f560000a88c095828e11d57a601069ce3</originalsourceid><addsrcrecordid>eNqVksFu1DAQhgMC0VI48ALIEhcqkdZOnMQ5VqvSgir1AufI60x2vXXsYDtq8_ZMsluEkPaAD4mlfPPN73iS5AOjFwzX5c5tLljOuXiZnDLOq5RWRfkK9zlnqaBVeZK8DWFHKatqJt4kJ5kQrBAFP32xun4aPISgnSWuI1bG0UtD1BRddE9a6TgRDwqG6Hwg0rbLpwdtgQzetaOKS6UlYFvXQ_Qaq58tD9oY8ESBMYFoSx6RwKeO29k5eg82ogY2Vlo1EeNCIM4T3Q9G2igXdSe1GT18WXrHLRD4n8AIDeD1sIUZWhvn2qPpDkHi3zEl2eqApmnudSzzu-R1J02A94f3WfLz6_WP1W16d3_zbXV1lyrOMpFWeUYzpmTb5nhXtAZWd2u8BJXRltGs6oqS4pJCKFoXIhPAWFtUsqSMlrWC_Cz5vPfin_81QohNr8McX1pwY2hYnVMuqkxUiH76B9250VtMh1QheCkEn6nzPaU8HsND1wxe99JPDaPNPFlYtWmWyUL248E4rnto_5DPo4TA5R541Aam46bm-_3NXvkbEmrh6g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1958468847</pqid></control><display><type>article</type><title>Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Fukui, Atsushi ; Funamizu, Ayano ; Fukuhara, Rie ; Shibahara, Hiroaki</creator><creatorcontrib>Fukui, Atsushi ; Funamizu, Ayano ; Fukuhara, Rie ; Shibahara, Hiroaki</creatorcontrib><description>Aim
Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women with recurrent pregnancy loss (RPL). We also investigated the expression of NCR in peripheral blood NK cells in pregnant women with and without a history of RPL.
Methods
The expression of NCR (NKp46, NKp44, and NKp30) in NK cells (CD56dim and CD56bright) in the uterine endometrium was analyzed using 3‐color flow cytometry. Cytokine (tumor necrosis factor‐α and interferon‐γ) production was also analyzed. NK cells from the mid‐secretory endometrium of 28 women with RPL, 34 women with implantation failure, and 74 controls were collected and mechanically dispersed using a tissue grinder. The expression of NCR in peripheral blood NK cells from pregnant women with (n = 17) and without (n = 91) a history of RPL was analyzed.
Results
The percentages of NKp46+ NK cells were significantly lower in both women with RPL and pregnant women with a history of RPL. The percentages of tumor necrosis factor‐α‐ and/or interferon‐γ‐producing uterine endometrial NK cells were significantly lower in women with RPL compared with controls.
Conclusion
The changes in NCR expression and cytokine production, especially decreased NKp46 expression in endometrial NK cells, suggests the presence of abnormal NK cell regulation in women with reproductive failures.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.13448</identifier><identifier>PMID: 28815854</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>Abortion, Habitual - blood ; Abortion, Habitual - immunology ; Abortion, Habitual - metabolism ; Adult ; cytokine ; Cytokines ; Cytokines - immunology ; Cytokines - metabolism ; Cytotoxicity ; Embryo Implantation ; Endometrium ; Endometrium - immunology ; Endometrium - metabolism ; Female ; Flow cytometry ; Humans ; Implantation ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; Lymphocyte receptors ; Miscarriage ; Natural killer cells ; NK cell ; NKp46 ; Peripheral blood ; Pregnancy ; Pregnancy - metabolism ; Receptors, Natural Cytotoxicity Triggering - immunology ; Receptors, Natural Cytotoxicity Triggering - metabolism ; recurrent pregnancy loss ; T cell receptors ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Uterus ; γ-Interferon</subject><ispartof>The journal of obstetrics and gynaecology research, 2017-11, Vol.43 (11), p.1678-1686</ispartof><rights>2017 Japan Society of Obstetrics and Gynecology</rights><rights>2017 Japan Society of Obstetrics and Gynecology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4128-732021cadd334409e19fb854c20d1027f560000a88c095828e11d57a601069ce3</citedby><cites>FETCH-LOGICAL-c4128-732021cadd334409e19fb854c20d1027f560000a88c095828e11d57a601069ce3</cites><orcidid>0000-0002-9059-7458</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.13448$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.13448$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28815854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukui, Atsushi</creatorcontrib><creatorcontrib>Funamizu, Ayano</creatorcontrib><creatorcontrib>Fukuhara, Rie</creatorcontrib><creatorcontrib>Shibahara, Hiroaki</creatorcontrib><title>Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J Obstet Gynaecol Res</addtitle><description>Aim
Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women with recurrent pregnancy loss (RPL). We also investigated the expression of NCR in peripheral blood NK cells in pregnant women with and without a history of RPL.
Methods
The expression of NCR (NKp46, NKp44, and NKp30) in NK cells (CD56dim and CD56bright) in the uterine endometrium was analyzed using 3‐color flow cytometry. Cytokine (tumor necrosis factor‐α and interferon‐γ) production was also analyzed. NK cells from the mid‐secretory endometrium of 28 women with RPL, 34 women with implantation failure, and 74 controls were collected and mechanically dispersed using a tissue grinder. The expression of NCR in peripheral blood NK cells from pregnant women with (n = 17) and without (n = 91) a history of RPL was analyzed.
Results
The percentages of NKp46+ NK cells were significantly lower in both women with RPL and pregnant women with a history of RPL. The percentages of tumor necrosis factor‐α‐ and/or interferon‐γ‐producing uterine endometrial NK cells were significantly lower in women with RPL compared with controls.
Conclusion
The changes in NCR expression and cytokine production, especially decreased NKp46 expression in endometrial NK cells, suggests the presence of abnormal NK cell regulation in women with reproductive failures.</description><subject>Abortion, Habitual - blood</subject><subject>Abortion, Habitual - immunology</subject><subject>Abortion, Habitual - metabolism</subject><subject>Adult</subject><subject>cytokine</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Cytokines - metabolism</subject><subject>Cytotoxicity</subject><subject>Embryo Implantation</subject><subject>Endometrium</subject><subject>Endometrium - immunology</subject><subject>Endometrium - metabolism</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Humans</subject><subject>Implantation</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Lymphocyte receptors</subject><subject>Miscarriage</subject><subject>Natural killer cells</subject><subject>NK cell</subject><subject>NKp46</subject><subject>Peripheral blood</subject><subject>Pregnancy</subject><subject>Pregnancy - metabolism</subject><subject>Receptors, Natural Cytotoxicity Triggering - immunology</subject><subject>Receptors, Natural Cytotoxicity Triggering - metabolism</subject><subject>recurrent pregnancy loss</subject><subject>T cell receptors</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Uterus</subject><subject>γ-Interferon</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVksFu1DAQhgMC0VI48ALIEhcqkdZOnMQ5VqvSgir1AufI60x2vXXsYDtq8_ZMsluEkPaAD4mlfPPN73iS5AOjFwzX5c5tLljOuXiZnDLOq5RWRfkK9zlnqaBVeZK8DWFHKatqJt4kJ5kQrBAFP32xun4aPISgnSWuI1bG0UtD1BRddE9a6TgRDwqG6Hwg0rbLpwdtgQzetaOKS6UlYFvXQ_Qaq58tD9oY8ESBMYFoSx6RwKeO29k5eg82ogY2Vlo1EeNCIM4T3Q9G2igXdSe1GT18WXrHLRD4n8AIDeD1sIUZWhvn2qPpDkHi3zEl2eqApmnudSzzu-R1J02A94f3WfLz6_WP1W16d3_zbXV1lyrOMpFWeUYzpmTb5nhXtAZWd2u8BJXRltGs6oqS4pJCKFoXIhPAWFtUsqSMlrWC_Cz5vPfin_81QohNr8McX1pwY2hYnVMuqkxUiH76B9250VtMh1QheCkEn6nzPaU8HsND1wxe99JPDaPNPFlYtWmWyUL248E4rnto_5DPo4TA5R541Aam46bm-_3NXvkbEmrh6g</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Fukui, Atsushi</creator><creator>Funamizu, Ayano</creator><creator>Fukuhara, Rie</creator><creator>Shibahara, Hiroaki</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9059-7458</orcidid></search><sort><creationdate>201711</creationdate><title>Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss</title><author>Fukui, Atsushi ; Funamizu, Ayano ; Fukuhara, Rie ; Shibahara, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4128-732021cadd334409e19fb854c20d1027f560000a88c095828e11d57a601069ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abortion, Habitual - blood</topic><topic>Abortion, Habitual - immunology</topic><topic>Abortion, Habitual - metabolism</topic><topic>Adult</topic><topic>cytokine</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Cytokines - metabolism</topic><topic>Cytotoxicity</topic><topic>Embryo Implantation</topic><topic>Endometrium</topic><topic>Endometrium - immunology</topic><topic>Endometrium - metabolism</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Humans</topic><topic>Implantation</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Lymphocyte receptors</topic><topic>Miscarriage</topic><topic>Natural killer cells</topic><topic>NK cell</topic><topic>NKp46</topic><topic>Peripheral blood</topic><topic>Pregnancy</topic><topic>Pregnancy - metabolism</topic><topic>Receptors, Natural Cytotoxicity Triggering - immunology</topic><topic>Receptors, Natural Cytotoxicity Triggering - metabolism</topic><topic>recurrent pregnancy loss</topic><topic>T cell receptors</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Uterus</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukui, Atsushi</creatorcontrib><creatorcontrib>Funamizu, Ayano</creatorcontrib><creatorcontrib>Fukuhara, Rie</creatorcontrib><creatorcontrib>Shibahara, Hiroaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukui, Atsushi</au><au>Funamizu, Ayano</au><au>Fukuhara, Rie</au><au>Shibahara, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J Obstet Gynaecol Res</addtitle><date>2017-11</date><risdate>2017</risdate><volume>43</volume><issue>11</issue><spage>1678</spage><epage>1686</epage><pages>1678-1686</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aim
Natural cytotoxicity receptors (NCR) are unique markers that regulate natural killer (NK) cell cytotoxicity and cytokine production. In this study, we investigated the expression of NCR (NKp46, NKp44, and NKp30) and cytokine production in NK cells derived from the uterine endometrium of women with recurrent pregnancy loss (RPL). We also investigated the expression of NCR in peripheral blood NK cells in pregnant women with and without a history of RPL.
Methods
The expression of NCR (NKp46, NKp44, and NKp30) in NK cells (CD56dim and CD56bright) in the uterine endometrium was analyzed using 3‐color flow cytometry. Cytokine (tumor necrosis factor‐α and interferon‐γ) production was also analyzed. NK cells from the mid‐secretory endometrium of 28 women with RPL, 34 women with implantation failure, and 74 controls were collected and mechanically dispersed using a tissue grinder. The expression of NCR in peripheral blood NK cells from pregnant women with (n = 17) and without (n = 91) a history of RPL was analyzed.
Results
The percentages of NKp46+ NK cells were significantly lower in both women with RPL and pregnant women with a history of RPL. The percentages of tumor necrosis factor‐α‐ and/or interferon‐γ‐producing uterine endometrial NK cells were significantly lower in women with RPL compared with controls.
Conclusion
The changes in NCR expression and cytokine production, especially decreased NKp46 expression in endometrial NK cells, suggests the presence of abnormal NK cell regulation in women with reproductive failures.</abstract><cop>Australia</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28815854</pmid><doi>10.1111/jog.13448</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9059-7458</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abortion, Habitual - blood Abortion, Habitual - immunology Abortion, Habitual - metabolism Adult cytokine Cytokines Cytokines - immunology Cytokines - metabolism Cytotoxicity Embryo Implantation Endometrium Endometrium - immunology Endometrium - metabolism Female Flow cytometry Humans Implantation Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Lymphocyte receptors Miscarriage Natural killer cells NK cell NKp46 Peripheral blood Pregnancy Pregnancy - metabolism Receptors, Natural Cytotoxicity Triggering - immunology Receptors, Natural Cytotoxicity Triggering - metabolism recurrent pregnancy loss T cell receptors Tumor necrosis factor Tumor necrosis factor-TNF Uterus γ-Interferon |
| title | Expression of natural cytotoxicity receptors and cytokine production on endometrial natural killer cells in women with recurrent pregnancy loss or implantation failure, and the expression of natural cytotoxicity receptors on peripheral blood natural killer cells in pregnant women with a history of recurrent pregnancy loss |
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