Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L
Key message Resistant and susceptible lines in Brassica rapa have different immune responses against Fusarium oxysporum inoculation. Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans ( Foc ) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to...
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| Veröffentlicht in: | Plant cell reports 2017-12, Vol.36 (12), p.1841-1854 |
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| creator | Miyaji, Naomi Shimizu, Motoki Miyazaki, Junji Osabe, Kenji Sato, Maho Ebe, Yusuke Takada, Satoko Kaji, Makoto Dennis, Elizabeth S. Fujimoto, Ryo Okazaki, Keiichi |
| description | Key message
Resistant and susceptible lines in
Brassica rapa
have different immune responses against
Fusarium oxysporum
inoculation.
Fusarium yellows caused by
Fusarium oxysporum
f. sp.
conglutinans
(
Foc
) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to
Foc
is still unknown. By comparing with and without
Foc
inoculation in both resistant and susceptible lines of Chinese cabbage (
Brassica rapa
var.
pekinensis
), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by
Foc
inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that
Foc
inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between
B. rapa
and
Arabidopsis thaliana
after
F. oxysporum
inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and
A. thaliana
. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against
F. oxysporum
. |
| doi_str_mv | 10.1007/s00299-017-2198-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1930478722</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1959136788</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-d2a68313699b81bd7db854ca1c1b745b4b32259fc37df01f6f28b26fff1ec233</originalsourceid><addsrcrecordid>eNp1kc2KFDEUhYMoTjv6AG4k4MZNaX6qK8lSG0eFBjezcBeSVCIZUkmZm2Ls1_CJzdDjD4KrG7jfOfeEg9BzSl5TQsQbIIQpNRAqBkaVHNQDtKMjZwMj_MtDtCOC0UEIOl6gJwA3hPSlmB6jCyYlVZMcd-jHoSyrqRFKxiXgVk0GV-PayuLxWkuIyQO2J3y1Qce2BZfvJ1hL7a-Yi9uSabFrrW-33uc_2MmnVG4BVw8RmskNmzxj2MD5tUWbPE4xd-uY8btqAKIzuJrV4ONT9CiYBP7Z_bxE11fvrw8fh-PnD58Ob4-DGyVrw8zMJDnlk1JWUjuL2cr96Ax11Ipxb0fLGdur4LiYA6FhCkxaNoUQqHeM80v06mzbf_lt89D0Enu4lEz2ZQNNFSejkIKxjr78B70pW809XKf2qmcQUnaKnilXC0D1Qa81LqaeNCX6ri997kv3vvRdX1p1zYt7580ufv6t-FVQB9gZgL7KX3396_R_XX8C9m6kLw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1959136788</pqid></control><display><type>article</type><title>Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Miyaji, Naomi ; Shimizu, Motoki ; Miyazaki, Junji ; Osabe, Kenji ; Sato, Maho ; Ebe, Yusuke ; Takada, Satoko ; Kaji, Makoto ; Dennis, Elizabeth S. ; Fujimoto, Ryo ; Okazaki, Keiichi</creator><creatorcontrib>Miyaji, Naomi ; Shimizu, Motoki ; Miyazaki, Junji ; Osabe, Kenji ; Sato, Maho ; Ebe, Yusuke ; Takada, Satoko ; Kaji, Makoto ; Dennis, Elizabeth S. ; Fujimoto, Ryo ; Okazaki, Keiichi</creatorcontrib><description>Key message
Resistant and susceptible lines in
Brassica rapa
have different immune responses against
Fusarium oxysporum
inoculation.
Fusarium yellows caused by
Fusarium oxysporum
f. sp.
conglutinans
(
Foc
) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to
Foc
is still unknown. By comparing with and without
Foc
inoculation in both resistant and susceptible lines of Chinese cabbage (
Brassica rapa
var.
pekinensis
), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by
Foc
inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that
Foc
inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between
B. rapa
and
Arabidopsis thaliana
after
F. oxysporum
inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and
A. thaliana
. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against
F. oxysporum
.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-017-2198-9</identifier><identifier>PMID: 28819684</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Brassica ; Brassica - drug effects ; Brassica - genetics ; Brassica - microbiology ; Brassica oleracea ; Brassica rapa ; Brassica rapa - drug effects ; Brassica rapa - genetics ; Brassica rapa - microbiology ; Cell Biology ; Chinese cabbage ; Chitin ; Chitin - pharmacology ; Ethylenes - pharmacology ; Fusarium ; Fusarium - physiology ; Fusarium oxysporum ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Plant - physiology ; Genes ; Genotype & phenotype ; Host plants ; Immune response ; Inoculation ; Life Sciences ; Original Article ; Plant Biochemistry ; Plant diseases ; Plant Sciences ; Plants (botany) ; Polymerase chain reaction ; Transcriptome - genetics ; Tryptophan ; Yellows</subject><ispartof>Plant cell reports, 2017-12, Vol.36 (12), p.1841-1854</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Plant Cell Reports is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-d2a68313699b81bd7db854ca1c1b745b4b32259fc37df01f6f28b26fff1ec233</citedby><cites>FETCH-LOGICAL-c482t-d2a68313699b81bd7db854ca1c1b745b4b32259fc37df01f6f28b26fff1ec233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-017-2198-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-017-2198-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28819684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyaji, Naomi</creatorcontrib><creatorcontrib>Shimizu, Motoki</creatorcontrib><creatorcontrib>Miyazaki, Junji</creatorcontrib><creatorcontrib>Osabe, Kenji</creatorcontrib><creatorcontrib>Sato, Maho</creatorcontrib><creatorcontrib>Ebe, Yusuke</creatorcontrib><creatorcontrib>Takada, Satoko</creatorcontrib><creatorcontrib>Kaji, Makoto</creatorcontrib><creatorcontrib>Dennis, Elizabeth S.</creatorcontrib><creatorcontrib>Fujimoto, Ryo</creatorcontrib><creatorcontrib>Okazaki, Keiichi</creatorcontrib><title>Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
Resistant and susceptible lines in
Brassica rapa
have different immune responses against
Fusarium oxysporum
inoculation.
Fusarium yellows caused by
Fusarium oxysporum
f. sp.
conglutinans
(
Foc
) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to
Foc
is still unknown. By comparing with and without
Foc
inoculation in both resistant and susceptible lines of Chinese cabbage (
Brassica rapa
var.
pekinensis
), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by
Foc
inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that
Foc
inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between
B. rapa
and
Arabidopsis thaliana
after
F. oxysporum
inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and
A. thaliana
. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against
F. oxysporum
.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brassica</subject><subject>Brassica - drug effects</subject><subject>Brassica - genetics</subject><subject>Brassica - microbiology</subject><subject>Brassica oleracea</subject><subject>Brassica rapa</subject><subject>Brassica rapa - drug effects</subject><subject>Brassica rapa - genetics</subject><subject>Brassica rapa - microbiology</subject><subject>Cell Biology</subject><subject>Chinese cabbage</subject><subject>Chitin</subject><subject>Chitin - pharmacology</subject><subject>Ethylenes - pharmacology</subject><subject>Fusarium</subject><subject>Fusarium - physiology</subject><subject>Fusarium oxysporum</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genes</subject><subject>Genotype & phenotype</subject><subject>Host plants</subject><subject>Immune response</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant diseases</subject><subject>Plant Sciences</subject><subject>Plants (botany)</subject><subject>Polymerase chain reaction</subject><subject>Transcriptome - genetics</subject><subject>Tryptophan</subject><subject>Yellows</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc2KFDEUhYMoTjv6AG4k4MZNaX6qK8lSG0eFBjezcBeSVCIZUkmZm2Ls1_CJzdDjD4KrG7jfOfeEg9BzSl5TQsQbIIQpNRAqBkaVHNQDtKMjZwMj_MtDtCOC0UEIOl6gJwA3hPSlmB6jCyYlVZMcd-jHoSyrqRFKxiXgVk0GV-PayuLxWkuIyQO2J3y1Qce2BZfvJ1hL7a-Yi9uSabFrrW-33uc_2MmnVG4BVw8RmskNmzxj2MD5tUWbPE4xd-uY8btqAKIzuJrV4ONT9CiYBP7Z_bxE11fvrw8fh-PnD58Ob4-DGyVrw8zMJDnlk1JWUjuL2cr96Ax11Ipxb0fLGdur4LiYA6FhCkxaNoUQqHeM80v06mzbf_lt89D0Enu4lEz2ZQNNFSejkIKxjr78B70pW809XKf2qmcQUnaKnilXC0D1Qa81LqaeNCX6ri997kv3vvRdX1p1zYt7580ufv6t-FVQB9gZgL7KX3396_R_XX8C9m6kLw</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Miyaji, Naomi</creator><creator>Shimizu, Motoki</creator><creator>Miyazaki, Junji</creator><creator>Osabe, Kenji</creator><creator>Sato, Maho</creator><creator>Ebe, Yusuke</creator><creator>Takada, Satoko</creator><creator>Kaji, Makoto</creator><creator>Dennis, Elizabeth S.</creator><creator>Fujimoto, Ryo</creator><creator>Okazaki, Keiichi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L</title><author>Miyaji, Naomi ; Shimizu, Motoki ; Miyazaki, Junji ; Osabe, Kenji ; Sato, Maho ; Ebe, Yusuke ; Takada, Satoko ; Kaji, Makoto ; Dennis, Elizabeth S. ; Fujimoto, Ryo ; Okazaki, Keiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-d2a68313699b81bd7db854ca1c1b745b4b32259fc37df01f6f28b26fff1ec233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Brassica</topic><topic>Brassica - drug effects</topic><topic>Brassica - genetics</topic><topic>Brassica - microbiology</topic><topic>Brassica oleracea</topic><topic>Brassica rapa</topic><topic>Brassica rapa - drug effects</topic><topic>Brassica rapa - genetics</topic><topic>Brassica rapa - microbiology</topic><topic>Cell Biology</topic><topic>Chinese cabbage</topic><topic>Chitin</topic><topic>Chitin - pharmacology</topic><topic>Ethylenes - pharmacology</topic><topic>Fusarium</topic><topic>Fusarium - physiology</topic><topic>Fusarium oxysporum</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genes</topic><topic>Genotype & phenotype</topic><topic>Host plants</topic><topic>Immune response</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant diseases</topic><topic>Plant Sciences</topic><topic>Plants (botany)</topic><topic>Polymerase chain reaction</topic><topic>Transcriptome - genetics</topic><topic>Tryptophan</topic><topic>Yellows</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyaji, Naomi</creatorcontrib><creatorcontrib>Shimizu, Motoki</creatorcontrib><creatorcontrib>Miyazaki, Junji</creatorcontrib><creatorcontrib>Osabe, Kenji</creatorcontrib><creatorcontrib>Sato, Maho</creatorcontrib><creatorcontrib>Ebe, Yusuke</creatorcontrib><creatorcontrib>Takada, Satoko</creatorcontrib><creatorcontrib>Kaji, Makoto</creatorcontrib><creatorcontrib>Dennis, Elizabeth S.</creatorcontrib><creatorcontrib>Fujimoto, Ryo</creatorcontrib><creatorcontrib>Okazaki, Keiichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyaji, Naomi</au><au>Shimizu, Motoki</au><au>Miyazaki, Junji</au><au>Osabe, Kenji</au><au>Sato, Maho</au><au>Ebe, Yusuke</au><au>Takada, Satoko</au><au>Kaji, Makoto</au><au>Dennis, Elizabeth S.</au><au>Fujimoto, Ryo</au><au>Okazaki, Keiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>36</volume><issue>12</issue><spage>1841</spage><epage>1854</epage><pages>1841-1854</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
Resistant and susceptible lines in
Brassica rapa
have different immune responses against
Fusarium oxysporum
inoculation.
Fusarium yellows caused by
Fusarium oxysporum
f. sp.
conglutinans
(
Foc
) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to
Foc
is still unknown. By comparing with and without
Foc
inoculation in both resistant and susceptible lines of Chinese cabbage (
Brassica rapa
var.
pekinensis
), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by
Foc
inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that
Foc
inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between
B. rapa
and
Arabidopsis thaliana
after
F. oxysporum
inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and
A. thaliana
. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against
F. oxysporum
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28819684</pmid><doi>10.1007/s00299-017-2198-9</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2017-12, Vol.36 (12), p.1841-1854 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1930478722 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Biomedical and Life Sciences Biotechnology Brassica Brassica - drug effects Brassica - genetics Brassica - microbiology Brassica oleracea Brassica rapa Brassica rapa - drug effects Brassica rapa - genetics Brassica rapa - microbiology Cell Biology Chinese cabbage Chitin Chitin - pharmacology Ethylenes - pharmacology Fusarium Fusarium - physiology Fusarium oxysporum Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology Genes Genotype & phenotype Host plants Immune response Inoculation Life Sciences Original Article Plant Biochemistry Plant diseases Plant Sciences Plants (botany) Polymerase chain reaction Transcriptome - genetics Tryptophan Yellows |
| title | Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L |
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