Real-time qRT-PCR assay for the detection of miRNAs using bi-directional extension sequences

Highly specific detection of miRNAs was performed using a novel bi-directional extension (BDE) assay. After reverse transcription, the cDNA was hybridized to a uniquely designed specific BDE sequence; this cDNA-BDE hybrid forms the PCR template. The PCR template was amplified in a SYBR Green-based q...

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Veröffentlicht in:	Analytical biochemistry 2017-11, Vol.536, p.32-35
Hauptverfasser:	Kim, Kyung Jin, Kwak, Jiwon, Lee, Jae-Hoon, Lee, Soo Suk
Format:	Artikel
Sprache:	eng
Schlagworte:	Bi-directional extension (BDE) DNA, Complementary - genetics Humans microRNA (miRNA) MicroRNAs - genetics Poly(A) tailing assay Real-Time Polymerase Chain Reaction Reverse transcription RT-qPCR Sensitivity and Specificity
Online-Zugang:	Volltext
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