Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages
. A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resi...
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| Veröffentlicht in: | The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2017-08, Vol.40 (8), p.72-7, Article 72 |
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| creator | Uddin, Md. Hemayet Wang, Huabin Rogerson, Fraser M. Lee, Peter Vee-Sin Zhang, Xuehua |
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A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named “Jaffa”. The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1
α
(IL-1
α
) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1
α
treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques.
Graphical abstract |
| doi_str_mv | 10.1140/epje/i2017-11561-1 |
| format | Article |
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A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named “Jaffa”. The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1
α
(IL-1
α
) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1
α
treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques.
Graphical abstract</description><identifier>ISSN: 1292-8941</identifier><identifier>EISSN: 1292-895X</identifier><identifier>DOI: 10.1140/epje/i2017-11561-1</identifier><identifier>PMID: 28803430</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aggrecans - chemistry ; Aggrecans - genetics ; Aggrecans - metabolism ; Animals ; Arthritis ; Atomic force microscopy ; Biological and Medical Physics ; Biophysics ; Cartilage ; Cartilage, Articular - chemistry ; Cartilage, Articular - metabolism ; Cartilage, Articular - ultrastructure ; Complex Fluids and Microfluidics ; Complex Systems ; Condensed matter physics ; Degradation ; Elasticity ; Endopeptidases - metabolism ; Genetic modification ; Interleukin-1 - metabolism ; Interleukins ; Mechanical properties ; Mice ; Morphology ; Mutation ; Nanostructure ; Nanotechnology ; Pathogenesis ; Physics ; Physics and Astronomy ; Polymer Sciences ; Proteolysis ; Regular Article ; Rodents ; Soft and Granular Matter ; Stiffness ; Structural stability ; Surfaces and Interfaces ; Thin Films</subject><ispartof>The European physical journal. E, Soft matter and biological physics, 2017-08, Vol.40 (8), p.72-7, Article 72</ispartof><rights>EDP Sciences, SIF, Springer-Verlag GmbH Germany 2017</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-cd49b3ae5c6a73342000824009e1c510a3c10333cbc1cec11d64c46d1c62a94d3</citedby><cites>FETCH-LOGICAL-c441t-cd49b3ae5c6a73342000824009e1c510a3c10333cbc1cec11d64c46d1c62a94d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1140/epje/i2017-11561-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1140/epje/i2017-11561-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28803430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uddin, Md. Hemayet</creatorcontrib><creatorcontrib>Wang, Huabin</creatorcontrib><creatorcontrib>Rogerson, Fraser M.</creatorcontrib><creatorcontrib>Lee, Peter Vee-Sin</creatorcontrib><creatorcontrib>Zhang, Xuehua</creatorcontrib><title>Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages</title><title>The European physical journal. E, Soft matter and biological physics</title><addtitle>Eur. Phys. J. E</addtitle><addtitle>Eur Phys J E Soft Matter</addtitle><description>.
A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named “Jaffa”. The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1
α
(IL-1
α
) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1
α
treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques.
Graphical abstract</description><subject>Aggrecans - chemistry</subject><subject>Aggrecans - genetics</subject><subject>Aggrecans - metabolism</subject><subject>Animals</subject><subject>Arthritis</subject><subject>Atomic force microscopy</subject><subject>Biological and Medical Physics</subject><subject>Biophysics</subject><subject>Cartilage</subject><subject>Cartilage, Articular - chemistry</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - ultrastructure</subject><subject>Complex Fluids and Microfluidics</subject><subject>Complex Systems</subject><subject>Condensed matter physics</subject><subject>Degradation</subject><subject>Elasticity</subject><subject>Endopeptidases - metabolism</subject><subject>Genetic modification</subject><subject>Interleukin-1 - metabolism</subject><subject>Interleukins</subject><subject>Mechanical properties</subject><subject>Mice</subject><subject>Morphology</subject><subject>Mutation</subject><subject>Nanostructure</subject><subject>Nanotechnology</subject><subject>Pathogenesis</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Polymer Sciences</subject><subject>Proteolysis</subject><subject>Regular Article</subject><subject>Rodents</subject><subject>Soft and Granular Matter</subject><subject>Stiffness</subject><subject>Structural stability</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><issn>1292-8941</issn><issn>1292-895X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9qFTEQxhdRbK2-gBey4E1v1maS7L9LKbUKBW8s9C7kzM5uc8gma5JFzpP4uqZ72iKCV5Nhft83E76ieA_sE4BkF7Ts6cJwBm0FUDdQwYviFHjPq66v714-vyWcFG9i3DPGsky8Lk541zEhBTstfl-NI2GKpR_LmMy8Wp1oKPU0BULtvD1Ek4eudLmJqC2Vsw_Lvbd-MrkttRvKmfBeu61dgl8oJEOb4y9jhyodFtqwJ1MdqQqUfZN2qZzXYzFIJeostXqi-LZ4NWob6d1jPStuv1z9uPxa3Xy__nb5-aZCKSFVOMh-JzTV2OhWCMnzHzsuGesJsAamBQITQuAOAQkBhkaibAbAhuteDuKsOD_65sN_rhSTmk1EslY78mtU0POu7URXs4x-_Afd-zW4fN1GQQtt32eKHykMPsZAo1qCmXU4KGDqITb1EJvaYlNbbAqy6MOj9bqbaXiWPOWUAXEEYh65icJfu_9v-wfCEagw</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Uddin, Md. Hemayet</creator><creator>Wang, Huabin</creator><creator>Rogerson, Fraser M.</creator><creator>Lee, Peter Vee-Sin</creator><creator>Zhang, Xuehua</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages</title><author>Uddin, Md. Hemayet ; Wang, Huabin ; Rogerson, Fraser M. ; Lee, Peter Vee-Sin ; Zhang, Xuehua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-cd49b3ae5c6a73342000824009e1c510a3c10333cbc1cec11d64c46d1c62a94d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aggrecans - chemistry</topic><topic>Aggrecans - genetics</topic><topic>Aggrecans - metabolism</topic><topic>Animals</topic><topic>Arthritis</topic><topic>Atomic force microscopy</topic><topic>Biological and Medical Physics</topic><topic>Biophysics</topic><topic>Cartilage</topic><topic>Cartilage, Articular - chemistry</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cartilage, Articular - ultrastructure</topic><topic>Complex Fluids and Microfluidics</topic><topic>Complex Systems</topic><topic>Condensed matter physics</topic><topic>Degradation</topic><topic>Elasticity</topic><topic>Endopeptidases - metabolism</topic><topic>Genetic modification</topic><topic>Interleukin-1 - metabolism</topic><topic>Interleukins</topic><topic>Mechanical properties</topic><topic>Mice</topic><topic>Morphology</topic><topic>Mutation</topic><topic>Nanostructure</topic><topic>Nanotechnology</topic><topic>Pathogenesis</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><topic>Polymer Sciences</topic><topic>Proteolysis</topic><topic>Regular Article</topic><topic>Rodents</topic><topic>Soft and Granular Matter</topic><topic>Stiffness</topic><topic>Structural stability</topic><topic>Surfaces and Interfaces</topic><topic>Thin Films</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uddin, Md. Hemayet</creatorcontrib><creatorcontrib>Wang, Huabin</creatorcontrib><creatorcontrib>Rogerson, Fraser M.</creatorcontrib><creatorcontrib>Lee, Peter Vee-Sin</creatorcontrib><creatorcontrib>Zhang, Xuehua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European physical journal. E, Soft matter and biological physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uddin, Md. Hemayet</au><au>Wang, Huabin</au><au>Rogerson, Fraser M.</au><au>Lee, Peter Vee-Sin</au><au>Zhang, Xuehua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages</atitle><jtitle>The European physical journal. E, Soft matter and biological physics</jtitle><stitle>Eur. Phys. J. E</stitle><addtitle>Eur Phys J E Soft Matter</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>40</volume><issue>8</issue><spage>72</spage><epage>7</epage><pages>72-7</pages><artnum>72</artnum><issn>1292-8941</issn><eissn>1292-895X</eissn><abstract>.
A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named “Jaffa”. The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1
α
(IL-1
α
) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1
α
treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28803430</pmid><doi>10.1140/epje/i2017-11561-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Aggrecans - chemistry Aggrecans - genetics Aggrecans - metabolism Animals Arthritis Atomic force microscopy Biological and Medical Physics Biophysics Cartilage Cartilage, Articular - chemistry Cartilage, Articular - metabolism Cartilage, Articular - ultrastructure Complex Fluids and Microfluidics Complex Systems Condensed matter physics Degradation Elasticity Endopeptidases - metabolism Genetic modification Interleukin-1 - metabolism Interleukins Mechanical properties Mice Morphology Mutation Nanostructure Nanotechnology Pathogenesis Physics Physics and Astronomy Polymer Sciences Proteolysis Regular Article Rodents Soft and Granular Matter Stiffness Structural stability Surfaces and Interfaces Thin Films |
| title | Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages |
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