Metagenomic alkaline protease from mangrove sediment

Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease‐producing clone from a metageno...

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Veröffentlicht in:	Journal of basic microbiology 2017-11, Vol.57 (11), p.962-973
Hauptverfasser:	Pessoa, Tharcilla B. A., Rezende, Rachel P., Marques, Eric de Lima Silva, Pirovani, Carlos P., dos Santos, Thalis F., dos Santos Gonçalves, Ana C., Romano, Carla C., Dotivo, Natielle C., Freitas, Ana C. O., Salay, Luiz C., Dias, João C. T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus - enzymology Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Biotechnology Brazil Chromatography, Gel cloning Endopeptidases - chemistry Endopeptidases - genetics Endopeptidases - isolation & purification Endopeptidases - metabolism Enzyme Activation Enzyme Assays Enzyme Stability Geologic Sediments - microbiology Hot Temperature Hydrogen-Ion Concentration Kinetics Metagenome Metagenomics Molecular Weight Peptide Hydrolases - chemistry Peptide Hydrolases - genetics Peptide Hydrolases - isolation & purification purification Salts Sequence Alignment Substrate Specificity Tandem Mass Spectrometry Temperature thermostability thermotolerance Wetlands
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