High-throughput screening of hybridoma supernatants using multiplexed fluorescent cell barcoding on live cells

With current available assay formats using either immobilized protein (ELISA, enzyme-linked immunosorbent assay) or immunostaining of fixed cells for primary monoclonal antibody (mAb) screening, researchers often fail to identify and characterize antibodies that recognize the native conformation of...

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Veröffentlicht in:	Journal of immunological methods 2017-12, Vol.451, p.20-27
Hauptverfasser:	Lu, Mei, Chan, Brian M., Schow, Peter W., Chang, Wesley S., King, Chadwick T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Monoclonal - metabolism Antibody Formation Antibody Specificity Antigens, Surface - genetics Antigens, Surface - immunology Barcode Binding Sites, Antibody Cell Separation - methods CHO Cells Cricetulus Female Flow cytometry Flow Cytometry - methods Fluorescent Dyes - chemistry HEK293 Cells High-throughput High-Throughput Screening Assays - methods Humans Hybridoma screening Hybridomas - immunology Hybridomas - metabolism Mice, Inbred C57BL Multiplex Predictive Value of Tests Protein Binding Reproducibility of Results Transfection
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