Shiitake ( Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in HEp-2 cells
Shiitake ( Lentinula edodes (Berkeley) Pegler) is one of the most consumed mushrooms, for both therapeutic purposes and as food, therefore, the study of its biological properties is of great interest for producers and consumers. Aqueous extracts of the shiitake mushroom ( L. edodes (Berkeley) Pegler...
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| Veröffentlicht in: | Toxicology in vitro 2006-12, Vol.20 (8), p.1555-1559 |
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| container_title | Toxicology in vitro |
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| creator | Miyaji, C.K. Poersch, A. Ribeiro, L.R. Eira, A.F. Cólus, I.M.S. |
| description | Shiitake (
Lentinula edodes (Berkeley) Pegler) is one of the most consumed mushrooms, for both therapeutic purposes and as food, therefore, the study of its biological properties is of great interest for producers and consumers. Aqueous extracts of the shiitake mushroom (
L. edodes (Berkeley) Pegler) were evaluated by the micronucleus test (MN) in HEp-2 cells
in vitro, to analyze their possible mutagenic and antimutagenic activities. None of the three extract concentrations tested (0.5, 1.0 and 1.5
mg/mL) presented mutagenicity at any of the preparation temperatures (4
°C, 22
±
2
°C and 60
°C). In the antimutagenicity evaluation, all extract concentrations at all preparation temperatures presented a strong protective activity for the HEp-2 cells in response to the alkylating agent methyl methanesulfonate (MMS) in the different treatment protocols: pre-treatment, simultaneous treatment and post-treatment. The extracts prepared at 22
±
2
°C presented the lowest frequencies of MN in the evaluations of mutagenicity and antimutagenicity, indicating these as the best option for potential therapeutic use. |
| doi_str_mv | 10.1016/j.tiv.2006.07.004 |
| format | Article |
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Lentinula edodes (Berkeley) Pegler) is one of the most consumed mushrooms, for both therapeutic purposes and as food, therefore, the study of its biological properties is of great interest for producers and consumers. Aqueous extracts of the shiitake mushroom (
L. edodes (Berkeley) Pegler) were evaluated by the micronucleus test (MN) in HEp-2 cells
in vitro, to analyze their possible mutagenic and antimutagenic activities. None of the three extract concentrations tested (0.5, 1.0 and 1.5
mg/mL) presented mutagenicity at any of the preparation temperatures (4
°C, 22
±
2
°C and 60
°C). In the antimutagenicity evaluation, all extract concentrations at all preparation temperatures presented a strong protective activity for the HEp-2 cells in response to the alkylating agent methyl methanesulfonate (MMS) in the different treatment protocols: pre-treatment, simultaneous treatment and post-treatment. The extracts prepared at 22
±
2
°C presented the lowest frequencies of MN in the evaluations of mutagenicity and antimutagenicity, indicating these as the best option for potential therapeutic use.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2006.07.004</identifier><identifier>PMID: 16949247</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Antimutagenic Agents - pharmacology ; Antimutagenicity ; Cell Line ; Cell Line, Tumor ; Eukaryotic Cells - drug effects ; Humans ; Lentinula edodes ; Methyl Methanesulfonate - pharmacology ; Micronucleus test ; Micronucleus Tests ; Mutagenicity ; Mutagens - chemistry ; Mutagens - toxicity ; Shiitake ; Shiitake Mushrooms - chemistry ; Temperature</subject><ispartof>Toxicology in vitro, 2006-12, Vol.20 (8), p.1555-1559</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-9097418d20c87e01a1a6bdbfa42d4c8cd87a55fed7e2da36e04a472d8892ddce3</citedby><cites>FETCH-LOGICAL-c382t-9097418d20c87e01a1a6bdbfa42d4c8cd87a55fed7e2da36e04a472d8892ddce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tiv.2006.07.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16949247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyaji, C.K.</creatorcontrib><creatorcontrib>Poersch, A.</creatorcontrib><creatorcontrib>Ribeiro, L.R.</creatorcontrib><creatorcontrib>Eira, A.F.</creatorcontrib><creatorcontrib>Cólus, I.M.S.</creatorcontrib><title>Shiitake ( Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in HEp-2 cells</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Shiitake (
Lentinula edodes (Berkeley) Pegler) is one of the most consumed mushrooms, for both therapeutic purposes and as food, therefore, the study of its biological properties is of great interest for producers and consumers. Aqueous extracts of the shiitake mushroom (
L. edodes (Berkeley) Pegler) were evaluated by the micronucleus test (MN) in HEp-2 cells
in vitro, to analyze their possible mutagenic and antimutagenic activities. None of the three extract concentrations tested (0.5, 1.0 and 1.5
mg/mL) presented mutagenicity at any of the preparation temperatures (4
°C, 22
±
2
°C and 60
°C). In the antimutagenicity evaluation, all extract concentrations at all preparation temperatures presented a strong protective activity for the HEp-2 cells in response to the alkylating agent methyl methanesulfonate (MMS) in the different treatment protocols: pre-treatment, simultaneous treatment and post-treatment. The extracts prepared at 22
±
2
°C presented the lowest frequencies of MN in the evaluations of mutagenicity and antimutagenicity, indicating these as the best option for potential therapeutic use.</description><subject>Antimutagenic Agents - pharmacology</subject><subject>Antimutagenicity</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Eukaryotic Cells - drug effects</subject><subject>Humans</subject><subject>Lentinula edodes</subject><subject>Methyl Methanesulfonate - pharmacology</subject><subject>Micronucleus test</subject><subject>Micronucleus Tests</subject><subject>Mutagenicity</subject><subject>Mutagens - chemistry</subject><subject>Mutagens - toxicity</subject><subject>Shiitake</subject><subject>Shiitake Mushrooms - chemistry</subject><subject>Temperature</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LJDEQhoO46Oj6A7xITjIeureS7umk8aTDqAsDu7DrOWSSas3YH2OSFv33ZpgBb0JBXZ73peoh5JxBzoBVv9Z5dG85B6hyEDlAeUAmTIo6K5gQh2QCUoqMF0VxTE5CWAPATHI4Isesqsual2JC2n_PzkX9gnRKl9hH14-tpmgHi4FOb9G_YIsfV_QvPrXoryi-R69NDFSnod1gEx4HT4eGds74oR9Ni4663o4Gbdr0YbHJODXYtuEn-dHoNuDZfp-Sx7vF__lDtvxz_3t-s8xMIXnMaqhFyaTlYKRAYJrpamVXjS65LY00Vgo9mzVoBXKriwqh1KXgVsqaW2uwOCWXu96NH15HDFF1Lmwv0D0OY1Cs5lLwYpZAtgPT5SF4bNTGu077D8VAbRWrtUqK1VaxAqGS4pS52JePqw7tV2LvNAHXOwDTi28OvQrGYZ90OI8mKju4b-o_AYmcjK0</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Miyaji, C.K.</creator><creator>Poersch, A.</creator><creator>Ribeiro, L.R.</creator><creator>Eira, A.F.</creator><creator>Cólus, I.M.S.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20061201</creationdate><title>Shiitake ( Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in HEp-2 cells</title><author>Miyaji, C.K. ; Poersch, A. ; Ribeiro, L.R. ; Eira, A.F. ; Cólus, I.M.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-9097418d20c87e01a1a6bdbfa42d4c8cd87a55fed7e2da36e04a472d8892ddce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antimutagenic Agents - pharmacology</topic><topic>Antimutagenicity</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Eukaryotic Cells - drug effects</topic><topic>Humans</topic><topic>Lentinula edodes</topic><topic>Methyl Methanesulfonate - pharmacology</topic><topic>Micronucleus test</topic><topic>Micronucleus Tests</topic><topic>Mutagenicity</topic><topic>Mutagens - chemistry</topic><topic>Mutagens - toxicity</topic><topic>Shiitake</topic><topic>Shiitake Mushrooms - chemistry</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miyaji, C.K.</creatorcontrib><creatorcontrib>Poersch, A.</creatorcontrib><creatorcontrib>Ribeiro, L.R.</creatorcontrib><creatorcontrib>Eira, A.F.</creatorcontrib><creatorcontrib>Cólus, I.M.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyaji, C.K.</au><au>Poersch, A.</au><au>Ribeiro, L.R.</au><au>Eira, A.F.</au><au>Cólus, I.M.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shiitake ( Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in HEp-2 cells</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>20</volume><issue>8</issue><spage>1555</spage><epage>1559</epage><pages>1555-1559</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>Shiitake (
Lentinula edodes (Berkeley) Pegler) is one of the most consumed mushrooms, for both therapeutic purposes and as food, therefore, the study of its biological properties is of great interest for producers and consumers. Aqueous extracts of the shiitake mushroom (
L. edodes (Berkeley) Pegler) were evaluated by the micronucleus test (MN) in HEp-2 cells
in vitro, to analyze their possible mutagenic and antimutagenic activities. None of the three extract concentrations tested (0.5, 1.0 and 1.5
mg/mL) presented mutagenicity at any of the preparation temperatures (4
°C, 22
±
2
°C and 60
°C). In the antimutagenicity evaluation, all extract concentrations at all preparation temperatures presented a strong protective activity for the HEp-2 cells in response to the alkylating agent methyl methanesulfonate (MMS) in the different treatment protocols: pre-treatment, simultaneous treatment and post-treatment. The extracts prepared at 22
±
2
°C presented the lowest frequencies of MN in the evaluations of mutagenicity and antimutagenicity, indicating these as the best option for potential therapeutic use.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16949247</pmid><doi>10.1016/j.tiv.2006.07.004</doi><tpages>5</tpages></addata></record> |
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| subjects | Antimutagenic Agents - pharmacology Antimutagenicity Cell Line Cell Line, Tumor Eukaryotic Cells - drug effects Humans Lentinula edodes Methyl Methanesulfonate - pharmacology Micronucleus test Micronucleus Tests Mutagenicity Mutagens - chemistry Mutagens - toxicity Shiitake Shiitake Mushrooms - chemistry Temperature |
| title | Shiitake ( Lentinula edodes (Berkeley) Pegler) extracts as a modulator of micronuclei induced in HEp-2 cells |
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