Solid-phase analytical derivatization for gas-chromatography–mass-spectrometry-based metabolomics

A novel derivatization method for gas chromatography/mass spectrometry (GC/MS)-based metabolomics was developed, based on solid-phase analytical derivatization (SPAD) with methoximation followed by trimethylsilylation. This SPAD method realized derivatization on solid phases combining strong anion e...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2017-12, Vol.124 (6), p.700-706
Hauptverfasser:	Takeo, Emi, Sasano, Ryoichi, Shimma, Shuichi, Bamba, Takeshi, Fukusaki, Eiichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetamides Amino acids Derivatization Fluoroacetates Gas Chromatography-Mass Spectrometry - methods Gas chromatography–mass spectrometry Hexanes Humans Metabolome Metabolomics Metabolomics - methods Organic acids Organic bases Sample preparation Solid phase Trimethylsilyl Compounds
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