Supplementation of lycopene in maturation media improves bovine embryo quality in vitro
This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-sta...
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| Veröffentlicht in: | Theriogenology 2017-11, Vol.103, p.173-184 |
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| creator | Chowdhury, M.M.R. Choi, Byung-Hyun Khan, Imran Lee, Kyeong-Lim Mesalam, Ayman Song, Seok-Hwan Xu, Lianguang Joo, Myeong-Don Afrin, Fahmida Kong, Il-Keun |
| description | This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P |
| doi_str_mv | 10.1016/j.theriogenology.2017.08.003 |
| format | Article |
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Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0.05), Immunocytochemistry confirmed that diminished protein expression of nuclear factor kappa B (NFκB), cyclooxygenase-2 (COX2), and 8-oxoguanine (indicated by ROS) and relative mRNA expression of the Caspase-3, NFκB, COX2, iNOS and BCL2-associated X (BAX) was significantly (P < 0.05) lower whereas the anti-apoptotic gene BCL2 was significantly (P < 0.05) higher in the 0.2 μM lycopene-supplemented group than the control. In conclusion, lycopene improves blastocyst quality by overcoming unfavorable conditions in in vitro culture systems.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2017.08.003</identifier><identifier>PMID: 28800556</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Apoptosis - drug effects ; Blastocyst - cytology ; Blastocyst - physiology ; Bovine ; Carotenoids - chemistry ; Carotenoids - pharmacology ; Cattle - embryology ; Culture Media ; Cumulus Cells ; Embryo culture ; Embryo Culture Techniques - veterinary ; Embryonic Development - drug effects ; Fertilization in Vitro ; Free radicals ; In Vitro Oocyte Maturation Techniques ; Lycopene ; Molecular Structure ; Oocytes - physiology ; Oxidative stress ; Reactive Oxygen Species ; RNA, Messenger</subject><ispartof>Theriogenology, 2017-11, Vol.103, p.173-184</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-6a6d939db095a4e2349847f881625a7700df2c566f882741c8cbb6c9def51e0c3</citedby><cites>FETCH-LOGICAL-c386t-6a6d939db095a4e2349847f881625a7700df2c566f882741c8cbb6c9def51e0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2017.08.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28800556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chowdhury, M.M.R.</creatorcontrib><creatorcontrib>Choi, Byung-Hyun</creatorcontrib><creatorcontrib>Khan, Imran</creatorcontrib><creatorcontrib>Lee, Kyeong-Lim</creatorcontrib><creatorcontrib>Mesalam, Ayman</creatorcontrib><creatorcontrib>Song, Seok-Hwan</creatorcontrib><creatorcontrib>Xu, Lianguang</creatorcontrib><creatorcontrib>Joo, Myeong-Don</creatorcontrib><creatorcontrib>Afrin, Fahmida</creatorcontrib><creatorcontrib>Kong, Il-Keun</creatorcontrib><title>Supplementation of lycopene in maturation media improves bovine embryo quality in vitro</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0.05), Immunocytochemistry confirmed that diminished protein expression of nuclear factor kappa B (NFκB), cyclooxygenase-2 (COX2), and 8-oxoguanine (indicated by ROS) and relative mRNA expression of the Caspase-3, NFκB, COX2, iNOS and BCL2-associated X (BAX) was significantly (P < 0.05) lower whereas the anti-apoptotic gene BCL2 was significantly (P < 0.05) higher in the 0.2 μM lycopene-supplemented group than the control. In conclusion, lycopene improves blastocyst quality by overcoming unfavorable conditions in in vitro culture systems.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - physiology</subject><subject>Bovine</subject><subject>Carotenoids - chemistry</subject><subject>Carotenoids - pharmacology</subject><subject>Cattle - embryology</subject><subject>Culture Media</subject><subject>Cumulus Cells</subject><subject>Embryo culture</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>Embryonic Development - drug effects</subject><subject>Fertilization in Vitro</subject><subject>Free radicals</subject><subject>In Vitro Oocyte Maturation Techniques</subject><subject>Lycopene</subject><subject>Molecular Structure</subject><subject>Oocytes - physiology</subject><subject>Oxidative stress</subject><subject>Reactive Oxygen Species</subject><subject>RNA, Messenger</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtq3TAQhkVpaE7SvkLxIotu7IwsW5agmxByg0AXSSE7IcvjVAfbciT5gN8mz5Ini8JJC9l1JRh9_1w-Qk4oFBQoP90W8Q966x5xcoN7XIsSaFOAKADYJ7KhopE5Kxn9TDYAkuVc0odDchTCFhLBOf1CDkshAOqab8jD3TLPA444RR2tmzLXZ8Nq3IwTZnbKRh0Xv_8ZsbM6s-Ps3Q5D1rqdTQyOrV9d9rTowcY1RV6edzZ695Uc9HoI-O39PSa_Ly_uz6_z219XN-dnt7lhgseca95JJrsWZK0rLFklRdX0QlBe1rppALq-NDXnqVQ2FTXCtC03ssO-pgiGHZMf-75pracFQ1SjDQaHQU_olqCoLEVNGat4Qn_uUeNdCB57NXs7ar8qCurNrdqqj27Vm1sFQiVzKf79fdLSJhf_wn9lJuByD2C6d2fRq2AsTiZ582ii6pz9v0mvqYuWZQ</recordid><startdate>201711</startdate><enddate>201711</enddate><creator>Chowdhury, M.M.R.</creator><creator>Choi, Byung-Hyun</creator><creator>Khan, Imran</creator><creator>Lee, Kyeong-Lim</creator><creator>Mesalam, Ayman</creator><creator>Song, Seok-Hwan</creator><creator>Xu, Lianguang</creator><creator>Joo, Myeong-Don</creator><creator>Afrin, Fahmida</creator><creator>Kong, Il-Keun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201711</creationdate><title>Supplementation of lycopene in maturation media improves bovine embryo quality in vitro</title><author>Chowdhury, M.M.R. ; Choi, Byung-Hyun ; Khan, Imran ; Lee, Kyeong-Lim ; Mesalam, Ayman ; Song, Seok-Hwan ; Xu, Lianguang ; Joo, Myeong-Don ; Afrin, Fahmida ; Kong, Il-Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-6a6d939db095a4e2349847f881625a7700df2c566f882741c8cbb6c9def51e0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - physiology</topic><topic>Bovine</topic><topic>Carotenoids - chemistry</topic><topic>Carotenoids - pharmacology</topic><topic>Cattle - embryology</topic><topic>Culture Media</topic><topic>Cumulus Cells</topic><topic>Embryo culture</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>Embryonic Development - drug effects</topic><topic>Fertilization in Vitro</topic><topic>Free radicals</topic><topic>In Vitro Oocyte Maturation Techniques</topic><topic>Lycopene</topic><topic>Molecular Structure</topic><topic>Oocytes - physiology</topic><topic>Oxidative stress</topic><topic>Reactive Oxygen Species</topic><topic>RNA, Messenger</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chowdhury, M.M.R.</creatorcontrib><creatorcontrib>Choi, Byung-Hyun</creatorcontrib><creatorcontrib>Khan, Imran</creatorcontrib><creatorcontrib>Lee, Kyeong-Lim</creatorcontrib><creatorcontrib>Mesalam, Ayman</creatorcontrib><creatorcontrib>Song, Seok-Hwan</creatorcontrib><creatorcontrib>Xu, Lianguang</creatorcontrib><creatorcontrib>Joo, Myeong-Don</creatorcontrib><creatorcontrib>Afrin, Fahmida</creatorcontrib><creatorcontrib>Kong, Il-Keun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chowdhury, M.M.R.</au><au>Choi, Byung-Hyun</au><au>Khan, Imran</au><au>Lee, Kyeong-Lim</au><au>Mesalam, Ayman</au><au>Song, Seok-Hwan</au><au>Xu, Lianguang</au><au>Joo, Myeong-Don</au><au>Afrin, Fahmida</au><au>Kong, Il-Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supplementation of lycopene in maturation media improves bovine embryo quality in vitro</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2017-11</date><risdate>2017</risdate><volume>103</volume><spage>173</spage><epage>184</epage><pages>173-184</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0.05), Immunocytochemistry confirmed that diminished protein expression of nuclear factor kappa B (NFκB), cyclooxygenase-2 (COX2), and 8-oxoguanine (indicated by ROS) and relative mRNA expression of the Caspase-3, NFκB, COX2, iNOS and BCL2-associated X (BAX) was significantly (P < 0.05) lower whereas the anti-apoptotic gene BCL2 was significantly (P < 0.05) higher in the 0.2 μM lycopene-supplemented group than the control. In conclusion, lycopene improves blastocyst quality by overcoming unfavorable conditions in in vitro culture systems.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28800556</pmid><doi>10.1016/j.theriogenology.2017.08.003</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Blastocyst - cytology Blastocyst - physiology Bovine Carotenoids - chemistry Carotenoids - pharmacology Cattle - embryology Culture Media Cumulus Cells Embryo culture Embryo Culture Techniques - veterinary Embryonic Development - drug effects Fertilization in Vitro Free radicals In Vitro Oocyte Maturation Techniques Lycopene Molecular Structure Oocytes - physiology Oxidative stress Reactive Oxygen Species RNA, Messenger |
| title | Supplementation of lycopene in maturation media improves bovine embryo quality in vitro |
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