6S RNA is involved in acid resistance and invasion of epithelial cells in Salmonella enterica serovar Typhimurium
Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood. We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimur...
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| Veröffentlicht in: | Future microbiology 2017-09, Vol.12 (12), p.1045-1057 |
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| creator | Ren, Jie Sang, Yu Qin, Ran Cui, Zhongli Yao, Yu-Feng |
| description | Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood.
We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium.
It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3.0. Additionally, genes in Salmonella pathogenicity island-1 were downregulated in the 6S RNA knockout strain. The loss of 6S RNA significantly reduced S. Typhimurium invasion ability in HeLa cells and virulence in a mouse model.
These findings demonstrate that 6S RNA plays an important role in S. Typhimurium survival under extremely acid conditions and for invasion of epithelial cells. |
| doi_str_mv | 10.2217/fmb-2017-0055 |
| format | Article |
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We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium.
It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3.0. Additionally, genes in Salmonella pathogenicity island-1 were downregulated in the 6S RNA knockout strain. The loss of 6S RNA significantly reduced S. Typhimurium invasion ability in HeLa cells and virulence in a mouse model.
These findings demonstrate that 6S RNA plays an important role in S. Typhimurium survival under extremely acid conditions and for invasion of epithelial cells.</description><identifier>ISSN: 1746-0913</identifier><identifier>EISSN: 1746-0921</identifier><identifier>DOI: 10.2217/fmb-2017-0055</identifier><identifier>PMID: 28796533</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Animals ; Antibiotics ; Bacteria ; Deoxyribonucleic acid ; Disease Models, Animal ; DNA ; E coli ; Epithelial cells ; Epithelial Cells - microbiology ; Epithelial Cells - pathology ; Gene expression ; Gene Expression Regulation, Bacterial ; Gene Knockout Techniques ; Genes, Bacterial - genetics ; Genomic Islands - genetics ; Gram-positive bacteria ; HeLa Cells ; Humans ; Hydrogen-Ion Concentration ; Infections ; Mice ; Mice, Inbred BALB C ; Pathogenicity ; Pathogens ; pH effects ; Plasmids ; Proteins ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA, Bacterial - genetics ; RNA, Bacterial - isolation & purification ; RNA, Bacterial - physiology ; RNA, Small Untranslated - genetics ; RNA, Small Untranslated - physiology ; RNA, Untranslated - genetics ; RNA, Untranslated - physiology ; Salmonella ; Salmonella enterica ; Salmonella typhimurium - genetics ; Salmonella typhimurium - pathogenicity ; Sequence Analysis, RNA - methods ; Serogroup ; Stress, Physiological - genetics ; Virulence ; Virulence - genetics</subject><ispartof>Future microbiology, 2017-09, Vol.12 (12), p.1045-1057</ispartof><rights>Copyright Future Medicine Ltd Sep 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-1e49703a854396e603329b7d97a72a50bd4850498b10cb2698afb1b38c8583973</citedby><cites>FETCH-LOGICAL-c321t-1e49703a854396e603329b7d97a72a50bd4850498b10cb2698afb1b38c8583973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28796533$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Jie</creatorcontrib><creatorcontrib>Sang, Yu</creatorcontrib><creatorcontrib>Qin, Ran</creatorcontrib><creatorcontrib>Cui, Zhongli</creatorcontrib><creatorcontrib>Yao, Yu-Feng</creatorcontrib><title>6S RNA is involved in acid resistance and invasion of epithelial cells in Salmonella enterica serovar Typhimurium</title><title>Future microbiology</title><addtitle>Future Microbiol</addtitle><description>Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood.
We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium.
It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3.0. Additionally, genes in Salmonella pathogenicity island-1 were downregulated in the 6S RNA knockout strain. The loss of 6S RNA significantly reduced S. Typhimurium invasion ability in HeLa cells and virulence in a mouse model.
These findings demonstrate that 6S RNA plays an important role in S. Typhimurium survival under extremely acid conditions and for invasion of epithelial cells.</description><subject>Animals</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Models, Animal</subject><subject>DNA</subject><subject>E coli</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - microbiology</subject><subject>Epithelial Cells - pathology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Knockout Techniques</subject><subject>Genes, Bacterial - genetics</subject><subject>Genomic Islands - genetics</subject><subject>Gram-positive bacteria</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infections</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>pH effects</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Bacterial - isolation & purification</subject><subject>RNA, Bacterial - physiology</subject><subject>RNA, Small Untranslated - genetics</subject><subject>RNA, Small Untranslated - physiology</subject><subject>RNA, Untranslated - genetics</subject><subject>RNA, Untranslated - physiology</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - pathogenicity</subject><subject>Sequence Analysis, RNA - methods</subject><subject>Serogroup</subject><subject>Stress, Physiological - genetics</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><issn>1746-0913</issn><issn>1746-0921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkc1LxDAQxYMorq4evUrAi5dqJmma5CjiF4iCu55D2qZslrbZTdqC_70tu-7B07wZfjM85iF0BeSOUhD3VZMnlIBICOH8CJ2BSLOEKArHBw1shs5jXI-EBAWnaEalUBln7AxtswX--njALmLXDr4ebDkKbApX4mCji51pC4tNO40HE51vsa-w3bhuZWtnalzYup6W8cLUjW_HzmDbdja4wuBogx9MwMufzco1fXB9c4FOKlNHe7mvc_T9_LR8fE3eP1_eHh_ek4JR6BKwqRKEGclTpjKbEcaoykWphBHUcJKXqeQkVTIHUuQ0U9JUOeRMFpJLpgSbo9vd3U3w297GTjcuTmZNa30fNSgqOUAGfERv_qFr34d2dKcpo1JSnhE1UsmOKoKPMdhKb4JrTPjRQPSUhR6z0FMWespi5K_3V_u8seWB_ns--wWBoIOH</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Ren, Jie</creator><creator>Sang, Yu</creator><creator>Qin, Ran</creator><creator>Cui, Zhongli</creator><creator>Yao, Yu-Feng</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>6S RNA is involved in acid resistance and invasion of epithelial cells in Salmonella enterica serovar Typhimurium</title><author>Ren, Jie ; Sang, Yu ; Qin, Ran ; Cui, Zhongli ; Yao, Yu-Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-1e49703a854396e603329b7d97a72a50bd4850498b10cb2698afb1b38c8583973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Deoxyribonucleic acid</topic><topic>Disease Models, Animal</topic><topic>DNA</topic><topic>E coli</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - microbiology</topic><topic>Epithelial Cells - pathology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Knockout Techniques</topic><topic>Genes, Bacterial - genetics</topic><topic>Genomic Islands - genetics</topic><topic>Gram-positive bacteria</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infections</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>pH effects</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Bacterial - isolation & purification</topic><topic>RNA, Bacterial - physiology</topic><topic>RNA, Small Untranslated - genetics</topic><topic>RNA, Small Untranslated - physiology</topic><topic>RNA, Untranslated - genetics</topic><topic>RNA, Untranslated - physiology</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - pathogenicity</topic><topic>Sequence Analysis, RNA - methods</topic><topic>Serogroup</topic><topic>Stress, Physiological - genetics</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Jie</creatorcontrib><creatorcontrib>Sang, Yu</creatorcontrib><creatorcontrib>Qin, Ran</creatorcontrib><creatorcontrib>Cui, Zhongli</creatorcontrib><creatorcontrib>Yao, Yu-Feng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Future microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Jie</au><au>Sang, Yu</au><au>Qin, Ran</au><au>Cui, Zhongli</au><au>Yao, Yu-Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>6S RNA is involved in acid resistance and invasion of epithelial cells in Salmonella enterica serovar Typhimurium</atitle><jtitle>Future microbiology</jtitle><addtitle>Future Microbiol</addtitle><date>2017-09</date><risdate>2017</risdate><volume>12</volume><issue>12</issue><spage>1045</spage><epage>1057</epage><pages>1045-1057</pages><issn>1746-0913</issn><eissn>1746-0921</eissn><abstract>Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood.
We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium.
It identified that 6S RNA encoded by the ssrS was significantly upregulated at pH 3.0. The 6S RNA knockout strain showed a reduced ability to survive at pH 3.0. Additionally, genes in Salmonella pathogenicity island-1 were downregulated in the 6S RNA knockout strain. The loss of 6S RNA significantly reduced S. Typhimurium invasion ability in HeLa cells and virulence in a mouse model.
These findings demonstrate that 6S RNA plays an important role in S. Typhimurium survival under extremely acid conditions and for invasion of epithelial cells.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>28796533</pmid><doi>10.2217/fmb-2017-0055</doi><tpages>13</tpages></addata></record> |
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| ispartof | Future microbiology, 2017-09, Vol.12 (12), p.1045-1057 |
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| subjects | Animals Antibiotics Bacteria Deoxyribonucleic acid Disease Models, Animal DNA E coli Epithelial cells Epithelial Cells - microbiology Epithelial Cells - pathology Gene expression Gene Expression Regulation, Bacterial Gene Knockout Techniques Genes, Bacterial - genetics Genomic Islands - genetics Gram-positive bacteria HeLa Cells Humans Hydrogen-Ion Concentration Infections Mice Mice, Inbred BALB C Pathogenicity Pathogens pH effects Plasmids Proteins Ribonucleic acid RNA RNA polymerase RNA, Bacterial - genetics RNA, Bacterial - isolation & purification RNA, Bacterial - physiology RNA, Small Untranslated - genetics RNA, Small Untranslated - physiology RNA, Untranslated - genetics RNA, Untranslated - physiology Salmonella Salmonella enterica Salmonella typhimurium - genetics Salmonella typhimurium - pathogenicity Sequence Analysis, RNA - methods Serogroup Stress, Physiological - genetics Virulence Virulence - genetics |
| title | 6S RNA is involved in acid resistance and invasion of epithelial cells in Salmonella enterica serovar Typhimurium |
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