Increased Activity and Involvement of Caspase-3 in Radiation-Induced Apoptosis in Neural Cells Precursors from Developing Rat Brain
Using primary cultures of neural precursor cells of cortex from developing rat brain, we demonstrated the involvement of caspase-3 in the apoptotic process induced by gamma irradiation. The precursor nature of cells was confirmed by nestin and GFAP immunoreactivity and by the capacity of differentia...
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| Veröffentlicht in: | Neurotoxicology (Park Forest South) 2004-03, Vol.25 (3), p.387-398 |
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| creator | Michelin, Severino del Rosario Perez, Maria Dubner, Diana Gisone, Pablo |
| description | Using primary cultures of neural precursor cells of cortex from developing rat brain, we demonstrated the involvement of caspase-3 in the apoptotic process induced by gamma irradiation. The precursor nature of cells was confirmed by nestin and GFAP immunoreactivity and by the capacity of differentiation in neuronal and glial cells after 5 days in culture.
Neural precursors were irradiated with single doses ranging from 0.1 to 4
Gy. Cellular death, determined 24
h post-irradiation (pi) was dose-dependent and the induction of apoptosis was confirmed by nuclear condensation, DNA fragmentation and hypodiploid DNA peak represented by the “sub G1” region. For the higher doses, apoptosis was evident after 4–6
h pi and increased during 24
h. Caspase-3 activity increased with doses and was maximal at 4–6
h pi with 3
Gy and remained similar with 4
Gy. The protection from radiation-induced apoptosis by caspase-3 inhibitor, zDEVD-fmk, confirmed that this enzyme is involved in the apoptotic mechanism in this system.
The possibility of using this tissue culture system for studying the effects of ionizing radiation on morphological and molecular differentiation was considered. |
| doi_str_mv | 10.1016/j.neuro.2003.08.009 |
| format | Article |
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Neural precursors were irradiated with single doses ranging from 0.1 to 4
Gy. Cellular death, determined 24
h post-irradiation (pi) was dose-dependent and the induction of apoptosis was confirmed by nuclear condensation, DNA fragmentation and hypodiploid DNA peak represented by the “sub G1” region. For the higher doses, apoptosis was evident after 4–6
h pi and increased during 24
h. Caspase-3 activity increased with doses and was maximal at 4–6
h pi with 3
Gy and remained similar with 4
Gy. The protection from radiation-induced apoptosis by caspase-3 inhibitor, zDEVD-fmk, confirmed that this enzyme is involved in the apoptotic mechanism in this system.
The possibility of using this tissue culture system for studying the effects of ionizing radiation on morphological and molecular differentiation was considered.</description><identifier>ISSN: 0161-813X</identifier><identifier>EISSN: 1872-9711</identifier><identifier>DOI: 10.1016/j.neuro.2003.08.009</identifier><identifier>PMID: 15019301</identifier><language>eng</language><publisher>Orlando, FL: Elsevier B.V</publisher><subject>Animals ; Apoptosis - radiation effects ; Biological and medical sciences ; Biological effects of radiation ; Caspase 3 ; Caspases - metabolism ; Caspases - radiation effects ; Cell Survival - radiation effects ; Cells, Cultured ; Cerebral Cortex - cytology ; Cerebral Cortex - enzymology ; Cerebral Cortex - radiation effects ; Developing rat brain ; Dose-Response Relationship, Radiation ; Embryo, Mammalian ; Enzyme Activation - radiation effects ; Fundamental and applied biological sciences. Psychology ; Gamma irradiation ; Gamma Rays ; Ionizing radiations ; Neurons - cytology ; Neurons - enzymology ; Neurons - radiation effects ; Rats ; Rats, Wistar ; Stem Cells - cytology ; Stem Cells - enzymology ; Stem Cells - radiation effects ; Tissues, organs and organisms biophysics</subject><ispartof>Neurotoxicology (Park Forest South), 2004-03, Vol.25 (3), p.387-398</ispartof><rights>2003 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-381eca937674e7a98a93a94864f5f74a045c979abd245a9607f93445cccb66aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neuro.2003.08.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15543810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15019301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Michelin, Severino</creatorcontrib><creatorcontrib>del Rosario Perez, Maria</creatorcontrib><creatorcontrib>Dubner, Diana</creatorcontrib><creatorcontrib>Gisone, Pablo</creatorcontrib><title>Increased Activity and Involvement of Caspase-3 in Radiation-Induced Apoptosis in Neural Cells Precursors from Developing Rat Brain</title><title>Neurotoxicology (Park Forest South)</title><addtitle>Neurotoxicology</addtitle><description>Using primary cultures of neural precursor cells of cortex from developing rat brain, we demonstrated the involvement of caspase-3 in the apoptotic process induced by gamma irradiation. The precursor nature of cells was confirmed by nestin and GFAP immunoreactivity and by the capacity of differentiation in neuronal and glial cells after 5 days in culture.
Neural precursors were irradiated with single doses ranging from 0.1 to 4
Gy. Cellular death, determined 24
h post-irradiation (pi) was dose-dependent and the induction of apoptosis was confirmed by nuclear condensation, DNA fragmentation and hypodiploid DNA peak represented by the “sub G1” region. For the higher doses, apoptosis was evident after 4–6
h pi and increased during 24
h. Caspase-3 activity increased with doses and was maximal at 4–6
h pi with 3
Gy and remained similar with 4
Gy. The protection from radiation-induced apoptosis by caspase-3 inhibitor, zDEVD-fmk, confirmed that this enzyme is involved in the apoptotic mechanism in this system.
The possibility of using this tissue culture system for studying the effects of ionizing radiation on morphological and molecular differentiation was considered.</description><subject>Animals</subject><subject>Apoptosis - radiation effects</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>Caspases - radiation effects</subject><subject>Cell Survival - radiation effects</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - enzymology</subject><subject>Cerebral Cortex - radiation effects</subject><subject>Developing rat brain</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Embryo, Mammalian</subject><subject>Enzyme Activation - radiation effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma irradiation</subject><subject>Gamma Rays</subject><subject>Ionizing radiations</subject><subject>Neurons - cytology</subject><subject>Neurons - enzymology</subject><subject>Neurons - radiation effects</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - enzymology</subject><subject>Stem Cells - radiation effects</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0161-813X</issn><issn>1872-9711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2v1CAUhonReMerv8DEsNFdK7S0lIWL6_g1yY0ao4k7coaeGiYtVKBN7to_LnUm0ZUrCDzv4eUh5ClnJWe8fXkqHS7BlxVjdcm6kjF1j-x4J6tCSc7vk12meNHx-vsVeRTjiTHeyFY9JFe8YVzVjO_Ir4MzASFiT29MsqtNdxRcTw9u9eOKE7pE_UD3EOcMFTW1jn6B3kKy3hUH1y9mi85-Tj7auF1_zK1gpHscx0g_BzRLiD5EOgQ_0Te44uhn637kMYm-DmDdY_JggDHik8t6Tb69e_t1_6G4_fT-sL-5LYzoqlTUHUcDqpatFChBdXkPSnStGJpBCmCiMUoqOPaVaEC1TA6qFvnQmGPbAtTX5MV57hz8zwVj0pONJtcEh36JmqtKMs5ZBuszaIKPMeCg52AnCHeaM7251yf9x73e3GvW6ew-p55dxi_HCfu_mYvsDDy_ABANjEMAZ2z8h2tE_uP2_Kszh1nGajHoaCy6LNpmm0n33v63yG_1v6Ru</recordid><startdate>20040301</startdate><enddate>20040301</enddate><creator>Michelin, Severino</creator><creator>del Rosario Perez, Maria</creator><creator>Dubner, Diana</creator><creator>Gisone, Pablo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20040301</creationdate><title>Increased Activity and Involvement of Caspase-3 in Radiation-Induced Apoptosis in Neural Cells Precursors from Developing Rat Brain</title><author>Michelin, Severino ; del Rosario Perez, Maria ; Dubner, Diana ; Gisone, Pablo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-381eca937674e7a98a93a94864f5f74a045c979abd245a9607f93445cccb66aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Apoptosis - radiation effects</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>Caspases - radiation effects</topic><topic>Cell Survival - radiation effects</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - enzymology</topic><topic>Cerebral Cortex - radiation effects</topic><topic>Developing rat brain</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Embryo, Mammalian</topic><topic>Enzyme Activation - radiation effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma irradiation</topic><topic>Gamma Rays</topic><topic>Ionizing radiations</topic><topic>Neurons - cytology</topic><topic>Neurons - enzymology</topic><topic>Neurons - radiation effects</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - enzymology</topic><topic>Stem Cells - radiation effects</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michelin, Severino</creatorcontrib><creatorcontrib>del Rosario Perez, Maria</creatorcontrib><creatorcontrib>Dubner, Diana</creatorcontrib><creatorcontrib>Gisone, Pablo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Neurotoxicology (Park Forest South)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michelin, Severino</au><au>del Rosario Perez, Maria</au><au>Dubner, Diana</au><au>Gisone, Pablo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Activity and Involvement of Caspase-3 in Radiation-Induced Apoptosis in Neural Cells Precursors from Developing Rat Brain</atitle><jtitle>Neurotoxicology (Park Forest South)</jtitle><addtitle>Neurotoxicology</addtitle><date>2004-03-01</date><risdate>2004</risdate><volume>25</volume><issue>3</issue><spage>387</spage><epage>398</epage><pages>387-398</pages><issn>0161-813X</issn><eissn>1872-9711</eissn><abstract>Using primary cultures of neural precursor cells of cortex from developing rat brain, we demonstrated the involvement of caspase-3 in the apoptotic process induced by gamma irradiation. The precursor nature of cells was confirmed by nestin and GFAP immunoreactivity and by the capacity of differentiation in neuronal and glial cells after 5 days in culture.
Neural precursors were irradiated with single doses ranging from 0.1 to 4
Gy. Cellular death, determined 24
h post-irradiation (pi) was dose-dependent and the induction of apoptosis was confirmed by nuclear condensation, DNA fragmentation and hypodiploid DNA peak represented by the “sub G1” region. For the higher doses, apoptosis was evident after 4–6
h pi and increased during 24
h. Caspase-3 activity increased with doses and was maximal at 4–6
h pi with 3
Gy and remained similar with 4
Gy. The protection from radiation-induced apoptosis by caspase-3 inhibitor, zDEVD-fmk, confirmed that this enzyme is involved in the apoptotic mechanism in this system.
The possibility of using this tissue culture system for studying the effects of ionizing radiation on morphological and molecular differentiation was considered.</abstract><cop>Orlando, FL</cop><pub>Elsevier B.V</pub><pmid>15019301</pmid><doi>10.1016/j.neuro.2003.08.009</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Apoptosis - radiation effects Biological and medical sciences Biological effects of radiation Caspase 3 Caspases - metabolism Caspases - radiation effects Cell Survival - radiation effects Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - enzymology Cerebral Cortex - radiation effects Developing rat brain Dose-Response Relationship, Radiation Embryo, Mammalian Enzyme Activation - radiation effects Fundamental and applied biological sciences. Psychology Gamma irradiation Gamma Rays Ionizing radiations Neurons - cytology Neurons - enzymology Neurons - radiation effects Rats Rats, Wistar Stem Cells - cytology Stem Cells - enzymology Stem Cells - radiation effects Tissues, organs and organisms biophysics |
| title | Increased Activity and Involvement of Caspase-3 in Radiation-Induced Apoptosis in Neural Cells Precursors from Developing Rat Brain |
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