Cryopreservation of protoplasts of the alga Porphyra yezoensis by vitrification
Protoplasts of Porphyra yezoensis were successfully cryopreserved at liquid nitrogen (LN) temperature by vitrification and subsequently regenerated into plants. The effects of different vitrification solutions, loading and dehydration time, and thawing condition on protoplasts viability after cryopr...
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| Veröffentlicht in: | Plant science (Limerick) 2004, Vol.166 (1), p.97-102 |
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| creator | Liu, Hongquan Yu, Wengong Dai, Jixun Gong, Qianhong Yang, Kunfeng Lu, Xinzhi |
| description | Protoplasts of
Porphyra yezoensis were successfully cryopreserved at liquid nitrogen (LN) temperature by vitrification and subsequently regenerated into plants. The effects of different vitrification solutions, loading and dehydration time, and thawing condition on protoplasts viability after cryopreservation were evaluated. VS6 (10% w/v dimethyl sulfoxide (DMSO), 30% w/v glycerol, 10% sucrose in seawater) seems to be a preferable vitrification solution for
P. yezoensis protoplasts. The best vitrification protocol using VS6 involves: loading with 25% VS6 for 5
min at 0
°C, dehydrating with ice-cold concentrated VS6 for 3
min, immediately immersing in LN, and then warming in 40
°C water bath. Using this protocol,
P. yezoensis protoplasts showed higher viability (66.5%) after cryopreservation. Although the regrowth of cryopreserved protoplasts showed a 2–3-day lag phase and recovered completely until 20 days after thawing, the growth pattern was the same as that of non-cryopreserved protoplasts. In summary, we have developed a new method for the cryopreservation of
P. yezoensis protoplasts, which allows long-term maintenance of valuable genotypes. |
| doi_str_mv | 10.1016/j.plantsci.2003.08.014 |
| format | Article |
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Porphyra yezoensis were successfully cryopreserved at liquid nitrogen (LN) temperature by vitrification and subsequently regenerated into plants. The effects of different vitrification solutions, loading and dehydration time, and thawing condition on protoplasts viability after cryopreservation were evaluated. VS6 (10% w/v dimethyl sulfoxide (DMSO), 30% w/v glycerol, 10% sucrose in seawater) seems to be a preferable vitrification solution for
P. yezoensis protoplasts. The best vitrification protocol using VS6 involves: loading with 25% VS6 for 5
min at 0
°C, dehydrating with ice-cold concentrated VS6 for 3
min, immediately immersing in LN, and then warming in 40
°C water bath. Using this protocol,
P. yezoensis protoplasts showed higher viability (66.5%) after cryopreservation. Although the regrowth of cryopreserved protoplasts showed a 2–3-day lag phase and recovered completely until 20 days after thawing, the growth pattern was the same as that of non-cryopreserved protoplasts. In summary, we have developed a new method for the cryopreservation of
P. yezoensis protoplasts, which allows long-term maintenance of valuable genotypes.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2003.08.014</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; algae and seaweeds ; Biological and medical sciences ; Cryopreservation ; drying ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. Plant material ; Genetic resources, diversity ; Genetics and breeding of economic plants ; in vitro regeneration ; Marine ; micropropagation ; Plant material ; plant regeneration ; Porphyra ; Porphyra yezoensis ; Protoplast ; protoplasts ; solutions ; temperature ; thawing ; Vitrification</subject><ispartof>Plant science (Limerick), 2004, Vol.166 (1), p.97-102</ispartof><rights>2003 Elsevier Ireland Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-92171c384dd4ace76f89e549cfa9bc26501cf58e88746725eca8c51c722a599e3</citedby><cites>FETCH-LOGICAL-c492t-92171c384dd4ace76f89e549cfa9bc26501cf58e88746725eca8c51c722a599e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945203003832$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15416782$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hongquan</creatorcontrib><creatorcontrib>Yu, Wengong</creatorcontrib><creatorcontrib>Dai, Jixun</creatorcontrib><creatorcontrib>Gong, Qianhong</creatorcontrib><creatorcontrib>Yang, Kunfeng</creatorcontrib><creatorcontrib>Lu, Xinzhi</creatorcontrib><title>Cryopreservation of protoplasts of the alga Porphyra yezoensis by vitrification</title><title>Plant science (Limerick)</title><description>Protoplasts of
Porphyra yezoensis were successfully cryopreserved at liquid nitrogen (LN) temperature by vitrification and subsequently regenerated into plants. The effects of different vitrification solutions, loading and dehydration time, and thawing condition on protoplasts viability after cryopreservation were evaluated. VS6 (10% w/v dimethyl sulfoxide (DMSO), 30% w/v glycerol, 10% sucrose in seawater) seems to be a preferable vitrification solution for
P. yezoensis protoplasts. The best vitrification protocol using VS6 involves: loading with 25% VS6 for 5
min at 0
°C, dehydrating with ice-cold concentrated VS6 for 3
min, immediately immersing in LN, and then warming in 40
°C water bath. Using this protocol,
P. yezoensis protoplasts showed higher viability (66.5%) after cryopreservation. Although the regrowth of cryopreserved protoplasts showed a 2–3-day lag phase and recovered completely until 20 days after thawing, the growth pattern was the same as that of non-cryopreserved protoplasts. In summary, we have developed a new method for the cryopreservation of
P. yezoensis protoplasts, which allows long-term maintenance of valuable genotypes.</description><subject>Agronomy. Soil science and plant productions</subject><subject>algae and seaweeds</subject><subject>Biological and medical sciences</subject><subject>Cryopreservation</subject><subject>drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetic resources, diversity</subject><subject>Genetics and breeding of economic plants</subject><subject>in vitro regeneration</subject><subject>Marine</subject><subject>micropropagation</subject><subject>Plant material</subject><subject>plant regeneration</subject><subject>Porphyra</subject><subject>Porphyra yezoensis</subject><subject>Protoplast</subject><subject>protoplasts</subject><subject>solutions</subject><subject>temperature</subject><subject>thawing</subject><subject>Vitrification</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkF1LHDEYhUNpodu1f6GdG3s303xOkjvLolYQFFqvQ8y-0SzjZEziwvTXm-kqXnoVAs857-FB6BvBHcGk_7nrpsGOJbvQUYxZh1WHCf-AVkRJ1lIq9Ee0qqBqNRf0M_qS8w5jTIWQK3S1SXOcEmRIe1tCHJvomynFEmtpLnn5lnto7HBnm-uYpvs52WaGfxHGHHJzOzf7UFLwwf2PH6FP3g4Zvr68a3Rzdvp387u9vDq_2Py6bB3XtLSaEkkcU3y75daB7L3SILh23upbR3uBifNCgVKS95IKcFY5QZyk1Aqtga3Rj0Nv3fr4BLmYh5AdDNUExKdsiKa9ZJy-DyrMGKW6gv0BdCnmnMCbKYUHm2ZDsFlEm515FW0W0QYrU0XX4PHLBZudHXyyowv5LS046aValnw_cN5GY-9SZW7-UEwYxpopoZemkwMB1dw-QDL1FowOtiGBK2Ybw3tjngGsXaG9</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Liu, Hongquan</creator><creator>Yu, Wengong</creator><creator>Dai, Jixun</creator><creator>Gong, Qianhong</creator><creator>Yang, Kunfeng</creator><creator>Lu, Xinzhi</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2004</creationdate><title>Cryopreservation of protoplasts of the alga Porphyra yezoensis by vitrification</title><author>Liu, Hongquan ; Yu, Wengong ; Dai, Jixun ; Gong, Qianhong ; Yang, Kunfeng ; Lu, Xinzhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-92171c384dd4ace76f89e549cfa9bc26501cf58e88746725eca8c51c722a599e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>algae and seaweeds</topic><topic>Biological and medical sciences</topic><topic>Cryopreservation</topic><topic>drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetic resources, diversity</topic><topic>Genetics and breeding of economic plants</topic><topic>in vitro regeneration</topic><topic>Marine</topic><topic>micropropagation</topic><topic>Plant material</topic><topic>plant regeneration</topic><topic>Porphyra</topic><topic>Porphyra yezoensis</topic><topic>Protoplast</topic><topic>protoplasts</topic><topic>solutions</topic><topic>temperature</topic><topic>thawing</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hongquan</creatorcontrib><creatorcontrib>Yu, Wengong</creatorcontrib><creatorcontrib>Dai, Jixun</creatorcontrib><creatorcontrib>Gong, Qianhong</creatorcontrib><creatorcontrib>Yang, Kunfeng</creatorcontrib><creatorcontrib>Lu, Xinzhi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hongquan</au><au>Yu, Wengong</au><au>Dai, Jixun</au><au>Gong, Qianhong</au><au>Yang, Kunfeng</au><au>Lu, Xinzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of protoplasts of the alga Porphyra yezoensis by vitrification</atitle><jtitle>Plant science (Limerick)</jtitle><date>2004</date><risdate>2004</risdate><volume>166</volume><issue>1</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>Protoplasts of
Porphyra yezoensis were successfully cryopreserved at liquid nitrogen (LN) temperature by vitrification and subsequently regenerated into plants. The effects of different vitrification solutions, loading and dehydration time, and thawing condition on protoplasts viability after cryopreservation were evaluated. VS6 (10% w/v dimethyl sulfoxide (DMSO), 30% w/v glycerol, 10% sucrose in seawater) seems to be a preferable vitrification solution for
P. yezoensis protoplasts. The best vitrification protocol using VS6 involves: loading with 25% VS6 for 5
min at 0
°C, dehydrating with ice-cold concentrated VS6 for 3
min, immediately immersing in LN, and then warming in 40
°C water bath. Using this protocol,
P. yezoensis protoplasts showed higher viability (66.5%) after cryopreservation. Although the regrowth of cryopreserved protoplasts showed a 2–3-day lag phase and recovered completely until 20 days after thawing, the growth pattern was the same as that of non-cryopreserved protoplasts. In summary, we have developed a new method for the cryopreservation of
P. yezoensis protoplasts, which allows long-term maintenance of valuable genotypes.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/j.plantsci.2003.08.014</doi><tpages>6</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions algae and seaweeds Biological and medical sciences Cryopreservation drying Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic resources, diversity Genetics and breeding of economic plants in vitro regeneration Marine micropropagation Plant material plant regeneration Porphyra Porphyra yezoensis Protoplast protoplasts solutions temperature thawing Vitrification |
| title | Cryopreservation of protoplasts of the alga Porphyra yezoensis by vitrification |
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