Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes
Exosomes are derived from various sources, including primary and cultured cell lines and body fluids. It is now evident that they are important for communication between cells. They have, therefore, been proposed as potential carriers to deliver drugs to specific sites. In this study, we examined st...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2017/08/01, Vol.40(8), pp.1183-1191 |
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| creator | Kumeda, Nahoko Ogawa, Yuko Akimoto, Yoshihiro Kawakami, Hayato Tsujimoto, Masafumi Yanoshita, Ryohei |
| description | Exosomes are derived from various sources, including primary and cultured cell lines and body fluids. It is now evident that they are important for communication between cells. They have, therefore, been proposed as potential carriers to deliver drugs to specific sites. In this study, we examined stability of exosomes derived from human saliva. Exosomes were stored at 4°C for up to 20 months and their membrane integrity assessed. Several exosomal markers, such as dipeptidyl peptidase IV (DPP IV; membrane marker) and programmed cell death 6-interacting protein (Alix, lumen marker), were retained intact after 20 months storage at 4°C. Moreover, intact exosomes could be isolated from whole saliva that had been stored at 4°C. Membrane disruption with detergents such as Triton X-100 and Nonidet P-40 caused partial solubilization of DPP IV and release of Alix into the supernatant. In contrast, sodium dodecyl sulfate treatment caused a complete disruption of the membrane. In addition, membrane stability was maintained after freezing and thawing. These results indicated that human saliva-derived exosomes are stable, maintaining their membrane integrity over a long storage period. |
| doi_str_mv | 10.1248/bpb.b16-00891 |
| format | Article |
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It is now evident that they are important for communication between cells. They have, therefore, been proposed as potential carriers to deliver drugs to specific sites. In this study, we examined stability of exosomes derived from human saliva. Exosomes were stored at 4°C for up to 20 months and their membrane integrity assessed. Several exosomal markers, such as dipeptidyl peptidase IV (DPP IV; membrane marker) and programmed cell death 6-interacting protein (Alix, lumen marker), were retained intact after 20 months storage at 4°C. Moreover, intact exosomes could be isolated from whole saliva that had been stored at 4°C. Membrane disruption with detergents such as Triton X-100 and Nonidet P-40 caused partial solubilization of DPP IV and release of Alix into the supernatant. In contrast, sodium dodecyl sulfate treatment caused a complete disruption of the membrane. In addition, membrane stability was maintained after freezing and thawing. These results indicated that human saliva-derived exosomes are stable, maintaining their membrane integrity over a long storage period.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b16-00891</identifier><identifier>PMID: 28768999</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Adult ; Apoptosis ; Body fluids ; Calcium-Binding Proteins - metabolism ; Cell Cycle Proteins - metabolism ; Cell death ; Cell lines ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cold Temperature ; detergent ; Detergents ; Detergents - pharmacology ; Dipeptidyl Peptidase 4 - metabolism ; Dipeptidyl-peptidase IV ; Endosomal Sorting Complexes Required for Transport - metabolism ; exosome ; Exosomes ; Exosomes - drug effects ; Exosomes - metabolism ; Freezing ; human whole saliva ; Humans ; Middle Aged ; Octoxynol - pharmacology ; Peptidase ; Polyethylene Glycols - pharmacology ; Saliva ; Saliva - cytology ; Sodium ; Sodium dodecyl sulfate ; Sodium lauryl sulfate ; Solubilization ; stability ; Storage ; Thawing ; Young Adult</subject><ispartof>Biological and Pharmaceutical Bulletin, 2017/08/01, Vol.40(8), pp.1183-1191</ispartof><rights>2017 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c609t-a40c980998a0ff8270614e6999b948eae8cd9976092d4fd53fb53edbef36c4a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28768999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumeda, Nahoko</creatorcontrib><creatorcontrib>Ogawa, Yuko</creatorcontrib><creatorcontrib>Akimoto, Yoshihiro</creatorcontrib><creatorcontrib>Kawakami, Hayato</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Yanoshita, Ryohei</creatorcontrib><creatorcontrib>Teikyo Heisei University</creatorcontrib><creatorcontrib>Kyorin University School of Medicine</creatorcontrib><creatorcontrib>bDepartment of Anatomy</creatorcontrib><creatorcontrib>aFaculty of Pharmaceutical Sciences</creatorcontrib><title>Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Exosomes are derived from various sources, including primary and cultured cell lines and body fluids. It is now evident that they are important for communication between cells. They have, therefore, been proposed as potential carriers to deliver drugs to specific sites. In this study, we examined stability of exosomes derived from human saliva. Exosomes were stored at 4°C for up to 20 months and their membrane integrity assessed. Several exosomal markers, such as dipeptidyl peptidase IV (DPP IV; membrane marker) and programmed cell death 6-interacting protein (Alix, lumen marker), were retained intact after 20 months storage at 4°C. Moreover, intact exosomes could be isolated from whole saliva that had been stored at 4°C. Membrane disruption with detergents such as Triton X-100 and Nonidet P-40 caused partial solubilization of DPP IV and release of Alix into the supernatant. In contrast, sodium dodecyl sulfate treatment caused a complete disruption of the membrane. In addition, membrane stability was maintained after freezing and thawing. These results indicated that human saliva-derived exosomes are stable, maintaining their membrane integrity over a long storage period.</description><subject>Adult</subject><subject>Apoptosis</subject><subject>Body fluids</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell death</subject><subject>Cell lines</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cold Temperature</subject><subject>detergent</subject><subject>Detergents</subject><subject>Detergents - pharmacology</subject><subject>Dipeptidyl Peptidase 4 - metabolism</subject><subject>Dipeptidyl-peptidase IV</subject><subject>Endosomal Sorting Complexes Required for Transport - metabolism</subject><subject>exosome</subject><subject>Exosomes</subject><subject>Exosomes - drug effects</subject><subject>Exosomes - metabolism</subject><subject>Freezing</subject><subject>human whole saliva</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Octoxynol - pharmacology</subject><subject>Peptidase</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Saliva</subject><subject>Saliva - cytology</subject><subject>Sodium</subject><subject>Sodium dodecyl sulfate</subject><subject>Sodium lauryl sulfate</subject><subject>Solubilization</subject><subject>stability</subject><subject>Storage</subject><subject>Thawing</subject><subject>Young Adult</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1P3DAQxa2qqGyhx16rSL1wCfgriX2sVhSQQD3AFVm2M1m8SuKtnVSlfz0Tlm6lXmYO8_ObN8-EfGb0nHGpLtzOnTtWl5Qqzd6RFROyKSvOqvdkRTVTZc0qdUw-5ryllDaUiw_kmKumVlrrFXlcP9lk_QQp_LFTiGMRu-IOBpfsCMXNOMEmhem5sGNb3MW0e4p93ARv--J-si70ywxfXM-DHYt724dfNj0Xl79jjgPkU3LU2T7Dp7d-Qh6-Xz6sr8vbH1c362-3pa-pnkorqdeKaq0s7TrFG1ozCTUadFoqsKB8q3WDLG9l11aic5WA1kEnai-tOCFne9ldij9nyJMZQvbQ93hDnLNhmldK10oqRL_-h27jnEY0t1A1b4RiAqlyT_kUc07QmV0KA15mGDVL7AZjNxi7eY0d-S9vqrMboD3Qf3NG4GoP4HRJL459GOHfbp8bFzBawylrUFSiLjZtGFMCi2ZcSy6oRKX1XmmbJ7uBwyqbpuB7eDUmqVFLORg8TD3-toFRvAA2M6zE</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>Kumeda, Nahoko</creator><creator>Ogawa, Yuko</creator><creator>Akimoto, Yoshihiro</creator><creator>Kawakami, Hayato</creator><creator>Tsujimoto, Masafumi</creator><creator>Yanoshita, Ryohei</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2017</creationdate><title>Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes</title><author>Kumeda, Nahoko ; Ogawa, Yuko ; Akimoto, Yoshihiro ; Kawakami, Hayato ; Tsujimoto, Masafumi ; Yanoshita, Ryohei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c609t-a40c980998a0ff8270614e6999b948eae8cd9976092d4fd53fb53edbef36c4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Apoptosis</topic><topic>Body fluids</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell death</topic><topic>Cell lines</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cold Temperature</topic><topic>detergent</topic><topic>Detergents</topic><topic>Detergents - pharmacology</topic><topic>Dipeptidyl Peptidase 4 - metabolism</topic><topic>Dipeptidyl-peptidase IV</topic><topic>Endosomal Sorting Complexes Required for Transport - metabolism</topic><topic>exosome</topic><topic>Exosomes</topic><topic>Exosomes - drug effects</topic><topic>Exosomes - metabolism</topic><topic>Freezing</topic><topic>human whole saliva</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Octoxynol - pharmacology</topic><topic>Peptidase</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Saliva</topic><topic>Saliva - cytology</topic><topic>Sodium</topic><topic>Sodium dodecyl sulfate</topic><topic>Sodium lauryl sulfate</topic><topic>Solubilization</topic><topic>stability</topic><topic>Storage</topic><topic>Thawing</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumeda, Nahoko</creatorcontrib><creatorcontrib>Ogawa, Yuko</creatorcontrib><creatorcontrib>Akimoto, Yoshihiro</creatorcontrib><creatorcontrib>Kawakami, Hayato</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Yanoshita, Ryohei</creatorcontrib><creatorcontrib>Teikyo Heisei University</creatorcontrib><creatorcontrib>Kyorin University School of Medicine</creatorcontrib><creatorcontrib>bDepartment of Anatomy</creatorcontrib><creatorcontrib>aFaculty of Pharmaceutical Sciences</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumeda, Nahoko</au><au>Ogawa, Yuko</au><au>Akimoto, Yoshihiro</au><au>Kawakami, Hayato</au><au>Tsujimoto, Masafumi</au><au>Yanoshita, Ryohei</au><aucorp>Teikyo Heisei University</aucorp><aucorp>Kyorin University School of Medicine</aucorp><aucorp>bDepartment of Anatomy</aucorp><aucorp>aFaculty of Pharmaceutical Sciences</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2017</date><risdate>2017</risdate><volume>40</volume><issue>8</issue><spage>1183</spage><epage>1191</epage><pages>1183-1191</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Exosomes are derived from various sources, including primary and cultured cell lines and body fluids. It is now evident that they are important for communication between cells. They have, therefore, been proposed as potential carriers to deliver drugs to specific sites. In this study, we examined stability of exosomes derived from human saliva. Exosomes were stored at 4°C for up to 20 months and their membrane integrity assessed. Several exosomal markers, such as dipeptidyl peptidase IV (DPP IV; membrane marker) and programmed cell death 6-interacting protein (Alix, lumen marker), were retained intact after 20 months storage at 4°C. Moreover, intact exosomes could be isolated from whole saliva that had been stored at 4°C. Membrane disruption with detergents such as Triton X-100 and Nonidet P-40 caused partial solubilization of DPP IV and release of Alix into the supernatant. In contrast, sodium dodecyl sulfate treatment caused a complete disruption of the membrane. In addition, membrane stability was maintained after freezing and thawing. These results indicated that human saliva-derived exosomes are stable, maintaining their membrane integrity over a long storage period.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>28768999</pmid><doi>10.1248/bpb.b16-00891</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Apoptosis Body fluids Calcium-Binding Proteins - metabolism Cell Cycle Proteins - metabolism Cell death Cell lines Cell Membrane - drug effects Cell Membrane - metabolism Cold Temperature detergent Detergents Detergents - pharmacology Dipeptidyl Peptidase 4 - metabolism Dipeptidyl-peptidase IV Endosomal Sorting Complexes Required for Transport - metabolism exosome Exosomes Exosomes - drug effects Exosomes - metabolism Freezing human whole saliva Humans Middle Aged Octoxynol - pharmacology Peptidase Polyethylene Glycols - pharmacology Saliva Saliva - cytology Sodium Sodium dodecyl sulfate Sodium lauryl sulfate Solubilization stability Storage Thawing Young Adult |
| title | Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes |
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