Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia
Summary Introduction Bronchial hyper‐responsiveness is usually measured with direct stimuli such as methacholine (MCh) or histamine. Adenosine 5′‐monophosphate (AMP), which acts indirectly via the secondary release of mediators, is another stimulus to measure bronchial hyper‐responsiveness. Aim To i...
Gespeichert in:
| Veröffentlicht in: | Clinical and experimental allergy 2004-01, Vol.34 (1), p.71-76 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 76 |
|---|---|
| container_issue | 1 |
| container_start_page | 71 |
| container_title | Clinical and experimental allergy |
| container_volume | 34 |
| creator | Van den berge, M. Kerstjens, H. A. M. De reus, D. M. Koëter, G. H. Kauffman, H. F. Postma, D. S. |
| description | Summary
Introduction
Bronchial hyper‐responsiveness is usually measured with direct stimuli such as methacholine (MCh) or histamine. Adenosine 5′‐monophosphate (AMP), which acts indirectly via the secondary release of mediators, is another stimulus to measure bronchial hyper‐responsiveness.
Aim
To investigate whether provocation with inhaled AMP itself initiates an inflammatory response resulting in an influx of eosinophils into the airway lumen.
Methods
We have included 21 non‐smoking atopic asthmatic subjects (mean FEV1 101% predicted, mean age 34 years). Each subject performed three sputum inductions on different days, at least seven days apart: one without previous provocation, one hour after PC20 methacholine, and one hour after PC20 AMP.
Results
After provocation with AMP, but not methacholine, the percentage of sputum eosinophils increased significantly (from 1.9±0.5% to 4.5±1% (P |
| doi_str_mv | 10.1111/j.1365-2222.2004.01832.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19258512</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>546106761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4912-a562c465396d16ba6b1647cca0d2982fdf4897a3dc5800c53a02566ac67368d63</originalsourceid><addsrcrecordid>eNqNkcFu1DAQhi0EokvhFZCFBKcm2E7s2AcO1aotSKVwAPVovI6jeEns1Hbo9sYz8Ug8CQ67aiVOzMWW_H2j8T8AQIxKnOvttsQVowXJVRKE6hJhXpFy9wis7h8egxUStC4aLuoj8CzGLUKoooI_BUe4bggijK7At8_B__BaJesdvLWph6o1zkfrDKS_f_4qRu_81Ps49SqZE7iZE3Q-wdGkXuneDxk8gda1szYRxmlO8wjN4mfLDlY9B086NUTz4nAeg6_nZ1_W74vLTxcf1qeXha4FJoWijOia0UqwFrONYhvM6kZrhVoiOOnaruaiUVWrKUdI00ohQhlTmjUV4y2rjsGbfd8p-JvZxCRHG7UZBuWMn6PEglBOMcngq3_ArZ-Dy7NlRogcZM0zxPeQDj7GYDo5BTuqcCcxkssK5FYuScslabmsQP5dgdxl9eWh_7wZTfsgHjLPwOsDoKJWQxeU0zY-cJQSjPnyo3d77tYO5u6_B5Drs9Pllv1i79uYzO7eV-G7zKE1VF5fXcg1PmfNR3Ylr6s_UvKxTg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>199918348</pqid></control><display><type>article</type><title>Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia</title><source>MEDLINE</source><source>Wiley Online Library Journals</source><creator>Van den berge, M. ; Kerstjens, H. A. M. ; De reus, D. M. ; Koëter, G. H. ; Kauffman, H. F. ; Postma, D. S.</creator><creatorcontrib>Van den berge, M. ; Kerstjens, H. A. M. ; De reus, D. M. ; Koëter, G. H. ; Kauffman, H. F. ; Postma, D. S.</creatorcontrib><description>Summary
Introduction
Bronchial hyper‐responsiveness is usually measured with direct stimuli such as methacholine (MCh) or histamine. Adenosine 5′‐monophosphate (AMP), which acts indirectly via the secondary release of mediators, is another stimulus to measure bronchial hyper‐responsiveness.
Aim
To investigate whether provocation with inhaled AMP itself initiates an inflammatory response resulting in an influx of eosinophils into the airway lumen.
Methods
We have included 21 non‐smoking atopic asthmatic subjects (mean FEV1 101% predicted, mean age 34 years). Each subject performed three sputum inductions on different days, at least seven days apart: one without previous provocation, one hour after PC20 methacholine, and one hour after PC20 AMP.
Results
After provocation with AMP, but not methacholine, the percentage of sputum eosinophils increased significantly (from 1.9±0.5% to 4.5±1% (P<0.01) and 1.9±0.5% (P=0.89)). No changes in the percentages of neutrophils, lymphocytes, macrophages, or bronchial epithelial cells were found.
Conclusion
A provocation test with AMP leads to an increased percentage of sputum eosinophils. This observation cannot be explained by a non‐specific response of the airways to a vigorous bronchoconstriction, since methacholine had no effect on inflammatory cells.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/j.1365-2222.2004.01832.x</identifier><identifier>PMID: 14720265</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>adenosine ; adenosine 5′-monophosphate ; Adenosine Monophosphate ; Administration, Inhalation ; Adult ; Allergic diseases ; Asthma - drug therapy ; Asthma - immunology ; Biological and medical sciences ; Bronchial Hyperreactivity - diagnosis ; Bronchial Provocation Tests ; Bronchoconstrictor Agents ; Cross-Over Studies ; Eosinophilia - chemically induced ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glucocorticoids - therapeutic use ; Humans ; hyper-responsiveness ; Immunopathology ; inflammation ; Male ; Medical sciences ; Methacholine Chloride ; sputum ; Sputum - immunology ; Statistics, Nonparametric</subject><ispartof>Clinical and experimental allergy, 2004-01, Vol.34 (1), p.71-76</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Jan 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4912-a562c465396d16ba6b1647cca0d2982fdf4897a3dc5800c53a02566ac67368d63</citedby><cites>FETCH-LOGICAL-c4912-a562c465396d16ba6b1647cca0d2982fdf4897a3dc5800c53a02566ac67368d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2222.2004.01832.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2222.2004.01832.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15521186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14720265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van den berge, M.</creatorcontrib><creatorcontrib>Kerstjens, H. A. M.</creatorcontrib><creatorcontrib>De reus, D. M.</creatorcontrib><creatorcontrib>Koëter, G. H.</creatorcontrib><creatorcontrib>Kauffman, H. F.</creatorcontrib><creatorcontrib>Postma, D. S.</creatorcontrib><title>Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Summary
Introduction
Bronchial hyper‐responsiveness is usually measured with direct stimuli such as methacholine (MCh) or histamine. Adenosine 5′‐monophosphate (AMP), which acts indirectly via the secondary release of mediators, is another stimulus to measure bronchial hyper‐responsiveness.
Aim
To investigate whether provocation with inhaled AMP itself initiates an inflammatory response resulting in an influx of eosinophils into the airway lumen.
Methods
We have included 21 non‐smoking atopic asthmatic subjects (mean FEV1 101% predicted, mean age 34 years). Each subject performed three sputum inductions on different days, at least seven days apart: one without previous provocation, one hour after PC20 methacholine, and one hour after PC20 AMP.
Results
After provocation with AMP, but not methacholine, the percentage of sputum eosinophils increased significantly (from 1.9±0.5% to 4.5±1% (P<0.01) and 1.9±0.5% (P=0.89)). No changes in the percentages of neutrophils, lymphocytes, macrophages, or bronchial epithelial cells were found.
Conclusion
A provocation test with AMP leads to an increased percentage of sputum eosinophils. This observation cannot be explained by a non‐specific response of the airways to a vigorous bronchoconstriction, since methacholine had no effect on inflammatory cells.</description><subject>adenosine</subject><subject>adenosine 5′-monophosphate</subject><subject>Adenosine Monophosphate</subject><subject>Administration, Inhalation</subject><subject>Adult</subject><subject>Allergic diseases</subject><subject>Asthma - drug therapy</subject><subject>Asthma - immunology</subject><subject>Biological and medical sciences</subject><subject>Bronchial Hyperreactivity - diagnosis</subject><subject>Bronchial Provocation Tests</subject><subject>Bronchoconstrictor Agents</subject><subject>Cross-Over Studies</subject><subject>Eosinophilia - chemically induced</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glucocorticoids - therapeutic use</subject><subject>Humans</subject><subject>hyper-responsiveness</subject><subject>Immunopathology</subject><subject>inflammation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methacholine Chloride</subject><subject>sputum</subject><subject>Sputum - immunology</subject><subject>Statistics, Nonparametric</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0EokvhFZCFBKcm2E7s2AcO1aotSKVwAPVovI6jeEns1Hbo9sYz8Ug8CQ67aiVOzMWW_H2j8T8AQIxKnOvttsQVowXJVRKE6hJhXpFy9wis7h8egxUStC4aLuoj8CzGLUKoooI_BUe4bggijK7At8_B__BaJesdvLWph6o1zkfrDKS_f_4qRu_81Ps49SqZE7iZE3Q-wdGkXuneDxk8gda1szYRxmlO8wjN4mfLDlY9B086NUTz4nAeg6_nZ1_W74vLTxcf1qeXha4FJoWijOia0UqwFrONYhvM6kZrhVoiOOnaruaiUVWrKUdI00ohQhlTmjUV4y2rjsGbfd8p-JvZxCRHG7UZBuWMn6PEglBOMcngq3_ArZ-Dy7NlRogcZM0zxPeQDj7GYDo5BTuqcCcxkssK5FYuScslabmsQP5dgdxl9eWh_7wZTfsgHjLPwOsDoKJWQxeU0zY-cJQSjPnyo3d77tYO5u6_B5Drs9Pllv1i79uYzO7eV-G7zKE1VF5fXcg1PmfNR3Ylr6s_UvKxTg</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Van den berge, M.</creator><creator>Kerstjens, H. A. M.</creator><creator>De reus, D. M.</creator><creator>Koëter, G. H.</creator><creator>Kauffman, H. F.</creator><creator>Postma, D. S.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope></search><sort><creationdate>200401</creationdate><title>Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia</title><author>Van den berge, M. ; Kerstjens, H. A. M. ; De reus, D. M. ; Koëter, G. H. ; Kauffman, H. F. ; Postma, D. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4912-a562c465396d16ba6b1647cca0d2982fdf4897a3dc5800c53a02566ac67368d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>adenosine</topic><topic>adenosine 5′-monophosphate</topic><topic>Adenosine Monophosphate</topic><topic>Administration, Inhalation</topic><topic>Adult</topic><topic>Allergic diseases</topic><topic>Asthma - drug therapy</topic><topic>Asthma - immunology</topic><topic>Biological and medical sciences</topic><topic>Bronchial Hyperreactivity - diagnosis</topic><topic>Bronchial Provocation Tests</topic><topic>Bronchoconstrictor Agents</topic><topic>Cross-Over Studies</topic><topic>Eosinophilia - chemically induced</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glucocorticoids - therapeutic use</topic><topic>Humans</topic><topic>hyper-responsiveness</topic><topic>Immunopathology</topic><topic>inflammation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methacholine Chloride</topic><topic>sputum</topic><topic>Sputum - immunology</topic><topic>Statistics, Nonparametric</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van den berge, M.</creatorcontrib><creatorcontrib>Kerstjens, H. A. M.</creatorcontrib><creatorcontrib>De reus, D. M.</creatorcontrib><creatorcontrib>Koëter, G. H.</creatorcontrib><creatorcontrib>Kauffman, H. F.</creatorcontrib><creatorcontrib>Postma, D. S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van den berge, M.</au><au>Kerstjens, H. A. M.</au><au>De reus, D. M.</au><au>Koëter, G. H.</au><au>Kauffman, H. F.</au><au>Postma, D. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2004-01</date><risdate>2004</risdate><volume>34</volume><issue>1</issue><spage>71</spage><epage>76</epage><pages>71-76</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Summary
Introduction
Bronchial hyper‐responsiveness is usually measured with direct stimuli such as methacholine (MCh) or histamine. Adenosine 5′‐monophosphate (AMP), which acts indirectly via the secondary release of mediators, is another stimulus to measure bronchial hyper‐responsiveness.
Aim
To investigate whether provocation with inhaled AMP itself initiates an inflammatory response resulting in an influx of eosinophils into the airway lumen.
Methods
We have included 21 non‐smoking atopic asthmatic subjects (mean FEV1 101% predicted, mean age 34 years). Each subject performed three sputum inductions on different days, at least seven days apart: one without previous provocation, one hour after PC20 methacholine, and one hour after PC20 AMP.
Results
After provocation with AMP, but not methacholine, the percentage of sputum eosinophils increased significantly (from 1.9±0.5% to 4.5±1% (P<0.01) and 1.9±0.5% (P=0.89)). No changes in the percentages of neutrophils, lymphocytes, macrophages, or bronchial epithelial cells were found.
Conclusion
A provocation test with AMP leads to an increased percentage of sputum eosinophils. This observation cannot be explained by a non‐specific response of the airways to a vigorous bronchoconstriction, since methacholine had no effect on inflammatory cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>14720265</pmid><doi>10.1111/j.1365-2222.2004.01832.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0954-7894 |
| ispartof | Clinical and experimental allergy, 2004-01, Vol.34 (1), p.71-76 |
| issn | 0954-7894 1365-2222 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19258512 |
| source | MEDLINE; Wiley Online Library Journals |
| subjects | adenosine adenosine 5′-monophosphate Adenosine Monophosphate Administration, Inhalation Adult Allergic diseases Asthma - drug therapy Asthma - immunology Biological and medical sciences Bronchial Hyperreactivity - diagnosis Bronchial Provocation Tests Bronchoconstrictor Agents Cross-Over Studies Eosinophilia - chemically induced Female Fundamental and applied biological sciences. Psychology Fundamental immunology Glucocorticoids - therapeutic use Humans hyper-responsiveness Immunopathology inflammation Male Medical sciences Methacholine Chloride sputum Sputum - immunology Statistics, Nonparametric |
| title | Provocation with adenosine 5′-monophosphate, but not methacholine, induces sputum eosinophilia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T14%3A45%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Provocation%20with%20adenosine%205%E2%80%B2-monophosphate,%20but%20not%20methacholine,%20induces%20sputum%20eosinophilia&rft.jtitle=Clinical%20and%20experimental%20allergy&rft.au=Van%20den%20berge,%20M.&rft.date=2004-01&rft.volume=34&rft.issue=1&rft.spage=71&rft.epage=76&rft.pages=71-76&rft.issn=0954-7894&rft.eissn=1365-2222&rft_id=info:doi/10.1111/j.1365-2222.2004.01832.x&rft_dat=%3Cproquest_cross%3E546106761%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=199918348&rft_id=info:pmid/14720265&rfr_iscdi=true |