Organization of Metallic Nanoparticles Using Tobacco Mosaic Virus Templates

Self-assembled cylindrical particles of wild type and recombinant tobacco mosaic virus (TMV) were used as organic templates for the controlled deposition and organization of Pt, Au, or Ag nanoparticles. Chemical reduction of [PtCl6]2- or [AuCl4]- complexes at acidic pH gave rise to the specific decoration of the external surface of wild-type TMV rods with metallic nanoparticles less than 10 nm in size. In contrast, photochemical reduction of Ag(I) salts at pH 7 resulted in nucleation and constrained growth of discrete Ag nanoparticles aligned within the 4 nm-wide internal channel. The number of encapsulated nanoparticles increased when Ag benzoate rather than Ag nitrate was used due to reduced supersaturation associated with the lower Ag/benzoate redox couple, which enhanced the surface-templating effect of the channel wall carboxylates compared with nucleation in solution. Similar experiments using a mutant TMV with reduced negative charge along the central cavity confirmed that glutamic and aspartate acid groups were involved in site-specific deposition. Our results suggest that it should be possible to prepare 1-D arrays for a wide range of inorganic quantum dots by molecular engineering of the internal and external surfaces of self-assembled TMV tubules.