Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer
BACKGROUND: Epithelial-mesenchymal transition (EMT) is one of the main events in colorectal cancer(CRC) spread. Snail-1 is a zinc transcription factor that mediates EMT in tumor cells probably by down-regulation of E-cadherin and claudin-1. OBJECTIVES: To detect the expression of epithelial markers...
Gespeichert in:
| Veröffentlicht in: | Cancer biomarkers : section A of Disease markers 2017-07, Vol.20 (1), p.107-122 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 122 |
|---|---|
| container_issue | 1 |
| container_start_page | 107 |
| container_title | Cancer biomarkers : section A of Disease markers |
| container_volume | 20 |
| creator | Rashed, Hayam E. Hussein, Samia Mosaad, Hala Abdelwahab, Mai M. Abdelhamid, Mohamed I. Mohamed, Salem Y. Mohamed, Abdel Motaleb Fayed, Alaa |
| description | BACKGROUND:
Epithelial-mesenchymal transition (EMT) is one of the main events in colorectal cancer(CRC) spread. Snail-1 is a zinc transcription factor that mediates EMT in tumor cells probably by down-regulation of E-cadherin and claudin-1.
OBJECTIVES:
To detect the expression of epithelial markers (claudin-1 and E-cadherin) and mesenchymal markers (snail-1 and vimentin) in primary cancer colon. Also, to select stage II cancer patients of a high risk that can benefit from postoperative adjuvant chemotherapy.
METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis were performed to investigate snail-1, claudin-1, E-cadherin and vimentin expressions at mRNA and protein levels in fresh tissues of cancer colon and normal colonic mucosa. The correlations between the expression of these markers and clinicopathological parameters were performed.
RESULTS:
Normal colonic mucosa revealed complete membranous expression of claudin-1, preserved E-cadherin and negative snail-1 and vimentin expressions. Compared to control, the expression of snail-1 and vimentin mRNA in cancer colon was significantly up-regulated while the expression of claudin-1 and E-cadherin mRNA was significantly down-regulated. These changes were significantly associated with stage and lymph node involvement at both mRNA and protein levels(
p
<
0.05). There were significant negative correlations between vimentin and each of E-cadherin and claudin-1 gene expression and between snail-1 and each of E-cadherin and claudin-1 gene expression. Moreover, these changes were independent predictors of recurrence of stage II cancer colon cases.
CONCLUSION:
There is a clinical significance of snail-1, claudin-1, E-cadherin and vimentin as possible markers for recognizing patients with lymph node involvement, advanced stage and high incidence of tumor recurrence in cancer colon. |
| doi_str_mv | 10.3233/CBM-170034 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_proquest_miscellaneous_1925281858</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3233_CBM-170034</sage_id><sourcerecordid>1925281858</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-f759eaed245c41f579f721e51c6bd9120cb4601a03ac99fa534e7b5880cdc3553</originalsourceid><addsrcrecordid>eNplkctOwzAQRS0EolDY8AEoEgsQUsDPJl5CxUsqggWsI9eZtC6JXexEol_Ab-M-QAg29ozmzJ0ZXYSOCL5glLHL4fVjSjKMGd9CeyTPRJoLSbdjLDKeYiJYD-2HMMOYM0LlLurRCEkp-B76fPZuYl1ojU6CmVhTGa2shsRVSTuFZAIWljVly1VumqazbmpC6_QUmgjXCXzMPYRgnF12wdxEsDaqThsIYPV00cTYQ61aKJNG-TfwITE20a6OLatx_gDtVKoOcLj5--j19uZleJ-Onu4ehlejVDOetWkV9wYFJeVCc1KJTFYZJSCIHoxLSSjWYz7ARGGmtJSVEoxDNhZ5jnWpmRCsj87WunPv3jsIbdGYoKGulQXXhYJIKmhOcpFH9OQPOnOdt3G7SEkms_jySJ2vKe1dCB6qYu5NPHJREFws7SmiPcXanggfbyS7cQPlD_rtRwRO10BQE_g177_UF33OmMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993971994</pqid></control><display><type>article</type><title>Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer</title><source>SAGE Open Access</source><creator>Rashed, Hayam E. ; Hussein, Samia ; Mosaad, Hala ; Abdelwahab, Mai M. ; Abdelhamid, Mohamed I. ; Mohamed, Salem Y. ; Mohamed, Abdel Motaleb ; Fayed, Alaa</creator><creatorcontrib>Rashed, Hayam E. ; Hussein, Samia ; Mosaad, Hala ; Abdelwahab, Mai M. ; Abdelhamid, Mohamed I. ; Mohamed, Salem Y. ; Mohamed, Abdel Motaleb ; Fayed, Alaa</creatorcontrib><description>BACKGROUND:
Epithelial-mesenchymal transition (EMT) is one of the main events in colorectal cancer(CRC) spread. Snail-1 is a zinc transcription factor that mediates EMT in tumor cells probably by down-regulation of E-cadherin and claudin-1.
OBJECTIVES:
To detect the expression of epithelial markers (claudin-1 and E-cadherin) and mesenchymal markers (snail-1 and vimentin) in primary cancer colon. Also, to select stage II cancer patients of a high risk that can benefit from postoperative adjuvant chemotherapy.
METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis were performed to investigate snail-1, claudin-1, E-cadherin and vimentin expressions at mRNA and protein levels in fresh tissues of cancer colon and normal colonic mucosa. The correlations between the expression of these markers and clinicopathological parameters were performed.
RESULTS:
Normal colonic mucosa revealed complete membranous expression of claudin-1, preserved E-cadherin and negative snail-1 and vimentin expressions. Compared to control, the expression of snail-1 and vimentin mRNA in cancer colon was significantly up-regulated while the expression of claudin-1 and E-cadherin mRNA was significantly down-regulated. These changes were significantly associated with stage and lymph node involvement at both mRNA and protein levels(
p
<
0.05). There were significant negative correlations between vimentin and each of E-cadherin and claudin-1 gene expression and between snail-1 and each of E-cadherin and claudin-1 gene expression. Moreover, these changes were independent predictors of recurrence of stage II cancer colon cases.
CONCLUSION:
There is a clinical significance of snail-1, claudin-1, E-cadherin and vimentin as possible markers for recognizing patients with lymph node involvement, advanced stage and high incidence of tumor recurrence in cancer colon.</description><identifier>ISSN: 1574-0153</identifier><identifier>EISSN: 1875-8592</identifier><identifier>DOI: 10.3233/CBM-170034</identifier><identifier>PMID: 28759954</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Adult ; Aged ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Cadherins - genetics ; Cadherins - metabolism ; Cancer ; Chemotherapy ; Claudin-1 - genetics ; Claudin-1 - metabolism ; Colon ; Colon cancer ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - surgery ; E-cadherin ; Epithelial-Mesenchymal Transition - genetics ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Health risks ; Humans ; Lymph ; Lymph nodes ; Lymphatic system ; Male ; Markers ; Mesenchyme ; Middle Aged ; Mucosa ; Neoplasm Recurrence, Local ; Patients ; Polymerase chain reaction ; Prognosis ; Reverse transcription ; Snail Family Transcription Factors - genetics ; Snail Family Transcription Factors - metabolism ; Tissues ; Tumor cells ; Vimentin ; Vimentin - genetics ; Vimentin - metabolism ; Young Adult ; Zinc</subject><ispartof>Cancer biomarkers : section A of Disease markers, 2017-07, Vol.20 (1), p.107-122</ispartof><rights>IOS Press and the authors. All rights reserved</rights><rights>Copyright IOS Press BV 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-f759eaed245c41f579f721e51c6bd9120cb4601a03ac99fa534e7b5880cdc3553</citedby><cites>FETCH-LOGICAL-c347t-f759eaed245c41f579f721e51c6bd9120cb4601a03ac99fa534e7b5880cdc3553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.3233/CBM-170034$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.3233/CBM-170034$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21957,27844,27915,27916,44936,45324</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.3233/CBM-170034?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28759954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rashed, Hayam E.</creatorcontrib><creatorcontrib>Hussein, Samia</creatorcontrib><creatorcontrib>Mosaad, Hala</creatorcontrib><creatorcontrib>Abdelwahab, Mai M.</creatorcontrib><creatorcontrib>Abdelhamid, Mohamed I.</creatorcontrib><creatorcontrib>Mohamed, Salem Y.</creatorcontrib><creatorcontrib>Mohamed, Abdel Motaleb</creatorcontrib><creatorcontrib>Fayed, Alaa</creatorcontrib><title>Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer</title><title>Cancer biomarkers : section A of Disease markers</title><addtitle>Cancer Biomark</addtitle><description>BACKGROUND:
Epithelial-mesenchymal transition (EMT) is one of the main events in colorectal cancer(CRC) spread. Snail-1 is a zinc transcription factor that mediates EMT in tumor cells probably by down-regulation of E-cadherin and claudin-1.
OBJECTIVES:
To detect the expression of epithelial markers (claudin-1 and E-cadherin) and mesenchymal markers (snail-1 and vimentin) in primary cancer colon. Also, to select stage II cancer patients of a high risk that can benefit from postoperative adjuvant chemotherapy.
METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis were performed to investigate snail-1, claudin-1, E-cadherin and vimentin expressions at mRNA and protein levels in fresh tissues of cancer colon and normal colonic mucosa. The correlations between the expression of these markers and clinicopathological parameters were performed.
RESULTS:
Normal colonic mucosa revealed complete membranous expression of claudin-1, preserved E-cadherin and negative snail-1 and vimentin expressions. Compared to control, the expression of snail-1 and vimentin mRNA in cancer colon was significantly up-regulated while the expression of claudin-1 and E-cadherin mRNA was significantly down-regulated. These changes were significantly associated with stage and lymph node involvement at both mRNA and protein levels(
p
<
0.05). There were significant negative correlations between vimentin and each of E-cadherin and claudin-1 gene expression and between snail-1 and each of E-cadherin and claudin-1 gene expression. Moreover, these changes were independent predictors of recurrence of stage II cancer colon cases.
CONCLUSION:
There is a clinical significance of snail-1, claudin-1, E-cadherin and vimentin as possible markers for recognizing patients with lymph node involvement, advanced stage and high incidence of tumor recurrence in cancer colon.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Cancer</subject><subject>Chemotherapy</subject><subject>Claudin-1 - genetics</subject><subject>Claudin-1 - metabolism</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - surgery</subject><subject>E-cadherin</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Health risks</subject><subject>Humans</subject><subject>Lymph</subject><subject>Lymph nodes</subject><subject>Lymphatic system</subject><subject>Male</subject><subject>Markers</subject><subject>Mesenchyme</subject><subject>Middle Aged</subject><subject>Mucosa</subject><subject>Neoplasm Recurrence, Local</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Prognosis</subject><subject>Reverse transcription</subject><subject>Snail Family Transcription Factors - genetics</subject><subject>Snail Family Transcription Factors - metabolism</subject><subject>Tissues</subject><subject>Tumor cells</subject><subject>Vimentin</subject><subject>Vimentin - genetics</subject><subject>Vimentin - metabolism</subject><subject>Young Adult</subject><subject>Zinc</subject><issn>1574-0153</issn><issn>1875-8592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkctOwzAQRS0EolDY8AEoEgsQUsDPJl5CxUsqggWsI9eZtC6JXexEol_Ab-M-QAg29ozmzJ0ZXYSOCL5glLHL4fVjSjKMGd9CeyTPRJoLSbdjLDKeYiJYD-2HMMOYM0LlLurRCEkp-B76fPZuYl1ojU6CmVhTGa2shsRVSTuFZAIWljVly1VumqazbmpC6_QUmgjXCXzMPYRgnF12wdxEsDaqThsIYPV00cTYQ61aKJNG-TfwITE20a6OLatx_gDtVKoOcLj5--j19uZleJ-Onu4ehlejVDOetWkV9wYFJeVCc1KJTFYZJSCIHoxLSSjWYz7ARGGmtJSVEoxDNhZ5jnWpmRCsj87WunPv3jsIbdGYoKGulQXXhYJIKmhOcpFH9OQPOnOdt3G7SEkms_jySJ2vKe1dCB6qYu5NPHJREFws7SmiPcXanggfbyS7cQPlD_rtRwRO10BQE_g177_UF33OmMg</recordid><startdate>20170719</startdate><enddate>20170719</enddate><creator>Rashed, Hayam E.</creator><creator>Hussein, Samia</creator><creator>Mosaad, Hala</creator><creator>Abdelwahab, Mai M.</creator><creator>Abdelhamid, Mohamed I.</creator><creator>Mohamed, Salem Y.</creator><creator>Mohamed, Abdel Motaleb</creator><creator>Fayed, Alaa</creator><general>SAGE Publications</general><general>IOS Press BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20170719</creationdate><title>Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer</title><author>Rashed, Hayam E. ; Hussein, Samia ; Mosaad, Hala ; Abdelwahab, Mai M. ; Abdelhamid, Mohamed I. ; Mohamed, Salem Y. ; Mohamed, Abdel Motaleb ; Fayed, Alaa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-f759eaed245c41f579f721e51c6bd9120cb4601a03ac99fa534e7b5880cdc3553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Cancer</topic><topic>Chemotherapy</topic><topic>Claudin-1 - genetics</topic><topic>Claudin-1 - metabolism</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - surgery</topic><topic>E-cadherin</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Health risks</topic><topic>Humans</topic><topic>Lymph</topic><topic>Lymph nodes</topic><topic>Lymphatic system</topic><topic>Male</topic><topic>Markers</topic><topic>Mesenchyme</topic><topic>Middle Aged</topic><topic>Mucosa</topic><topic>Neoplasm Recurrence, Local</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Prognosis</topic><topic>Reverse transcription</topic><topic>Snail Family Transcription Factors - genetics</topic><topic>Snail Family Transcription Factors - metabolism</topic><topic>Tissues</topic><topic>Tumor cells</topic><topic>Vimentin</topic><topic>Vimentin - genetics</topic><topic>Vimentin - metabolism</topic><topic>Young Adult</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rashed, Hayam E.</creatorcontrib><creatorcontrib>Hussein, Samia</creatorcontrib><creatorcontrib>Mosaad, Hala</creatorcontrib><creatorcontrib>Abdelwahab, Mai M.</creatorcontrib><creatorcontrib>Abdelhamid, Mohamed I.</creatorcontrib><creatorcontrib>Mohamed, Salem Y.</creatorcontrib><creatorcontrib>Mohamed, Abdel Motaleb</creatorcontrib><creatorcontrib>Fayed, Alaa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer biomarkers : section A of Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Rashed, Hayam E.</au><au>Hussein, Samia</au><au>Mosaad, Hala</au><au>Abdelwahab, Mai M.</au><au>Abdelhamid, Mohamed I.</au><au>Mohamed, Salem Y.</au><au>Mohamed, Abdel Motaleb</au><au>Fayed, Alaa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer</atitle><jtitle>Cancer biomarkers : section A of Disease markers</jtitle><addtitle>Cancer Biomark</addtitle><date>2017-07-19</date><risdate>2017</risdate><volume>20</volume><issue>1</issue><spage>107</spage><epage>122</epage><pages>107-122</pages><issn>1574-0153</issn><eissn>1875-8592</eissn><abstract>BACKGROUND:
Epithelial-mesenchymal transition (EMT) is one of the main events in colorectal cancer(CRC) spread. Snail-1 is a zinc transcription factor that mediates EMT in tumor cells probably by down-regulation of E-cadherin and claudin-1.
OBJECTIVES:
To detect the expression of epithelial markers (claudin-1 and E-cadherin) and mesenchymal markers (snail-1 and vimentin) in primary cancer colon. Also, to select stage II cancer patients of a high risk that can benefit from postoperative adjuvant chemotherapy.
METHODS:
Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis were performed to investigate snail-1, claudin-1, E-cadherin and vimentin expressions at mRNA and protein levels in fresh tissues of cancer colon and normal colonic mucosa. The correlations between the expression of these markers and clinicopathological parameters were performed.
RESULTS:
Normal colonic mucosa revealed complete membranous expression of claudin-1, preserved E-cadherin and negative snail-1 and vimentin expressions. Compared to control, the expression of snail-1 and vimentin mRNA in cancer colon was significantly up-regulated while the expression of claudin-1 and E-cadherin mRNA was significantly down-regulated. These changes were significantly associated with stage and lymph node involvement at both mRNA and protein levels(
p
<
0.05). There were significant negative correlations between vimentin and each of E-cadherin and claudin-1 gene expression and between snail-1 and each of E-cadherin and claudin-1 gene expression. Moreover, these changes were independent predictors of recurrence of stage II cancer colon cases.
CONCLUSION:
There is a clinical significance of snail-1, claudin-1, E-cadherin and vimentin as possible markers for recognizing patients with lymph node involvement, advanced stage and high incidence of tumor recurrence in cancer colon.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>28759954</pmid><doi>10.3233/CBM-170034</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1574-0153 |
| ispartof | Cancer biomarkers : section A of Disease markers, 2017-07, Vol.20 (1), p.107-122 |
| issn | 1574-0153 1875-8592 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1925281858 |
| source | SAGE Open Access |
| subjects | Adult Aged Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Cadherins - genetics Cadherins - metabolism Cancer Chemotherapy Claudin-1 - genetics Claudin-1 - metabolism Colon Colon cancer Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - surgery E-cadherin Epithelial-Mesenchymal Transition - genetics Female Gene expression Gene Expression Regulation, Neoplastic Health risks Humans Lymph Lymph nodes Lymphatic system Male Markers Mesenchyme Middle Aged Mucosa Neoplasm Recurrence, Local Patients Polymerase chain reaction Prognosis Reverse transcription Snail Family Transcription Factors - genetics Snail Family Transcription Factors - metabolism Tissues Tumor cells Vimentin Vimentin - genetics Vimentin - metabolism Young Adult Zinc |
| title | Prognostic significance of the genetic and the immunohistochemical expression of epithelial-mesenchymal-related markers in colon cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T18%3A31%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_AFRWT&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prognostic%20significance%20of%20the%20genetic%20and%20the%20immunohistochemical%20expression%20of%20epithelial-mesenchymal-related%20markers%20in%20colon%20cancer&rft.jtitle=Cancer%20biomarkers%20:%20section%20A%20of%20Disease%20markers&rft.au=Rashed,%20Hayam%20E.&rft.date=2017-07-19&rft.volume=20&rft.issue=1&rft.spage=107&rft.epage=122&rft.pages=107-122&rft.issn=1574-0153&rft.eissn=1875-8592&rft_id=info:doi/10.3233/CBM-170034&rft_dat=%3Cproquest_AFRWT%3E1925281858%3C/proquest_AFRWT%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1993971994&rft_id=info:pmid/28759954&rft_sage_id=10.3233_CBM-170034&rfr_iscdi=true |