New insights into the catalytic mechanism of human glycine N‐acyltransferase

Even though the glycine conjugation pathway was one of the first metabolic pathways to be discovered, this pathway remains very poorly characterized. The bi‐substrate kinetic parameters of a recombinant human glycine N‐acyltransferase (GLYAT, E.C. 2.3.1.13) were determined using the traditional colo...

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Veröffentlicht in:	Journal of biochemical and molecular toxicology 2017-11, Vol.31 (11), p.n/a
Hauptverfasser:	der Sluis, Rencia, Ungerer, Vida, Nortje, Carla, Dijk, Alberdina, Erasmus, Elardus
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyl Coenzyme A - metabolism Acyltransferases - chemistry Acyltransferases - genetics Acyltransferases - metabolism Allosteric properties Catalysis Chromatography, High Pressure Liquid - methods Colorimetry Colorimetry - methods Conjugation Enzyme kinetics enzyme mechanism Glycine Glycine - metabolism Glycine N-acyltransferase High-performance liquid chromatography Hippurates - analysis Hippurates - metabolism Hippuric acid HPLC–ESI‐MS/MS human Humans Kinetics Liquid chromatography Metabolic pathways Reaction mechanisms Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Solvents - chemistry Spectrometry, Mass, Electrospray Ionization - methods Substrate inhibition Tandem Mass Spectrometry - methods
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