Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library
Glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. If glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. While several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is i...
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| Veröffentlicht in: | Pesticide biochemistry and physiology 2017-08, Vol.140, p.65-68 |
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| container_title | Pesticide biochemistry and physiology |
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| creator | Tao, Bo Shao, Bai-Hui Qiao, Yu-Xin Wang, Xiao-Qin Chang, Shu-Jun Qiu, Li-Juan |
| description | Glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. If glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. While several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is interest in identifying additional genes for glyphosate tolerance. This research constructed a high-quality cDNA library form the glyphosate-resistant fungus Aspergillus oryzae RIB40 to identify genes that may confer resistance to glyphosate. Using a medium containing glyphosate (120mM), we screened several clones from the library. Based on a nucleotide sequence analysis, we identified a gene of unknown function (GenBank accession number: XM_001826835.2) that encoded a hypothetical 344-amino acid protein. The gene was named MFS40. Its ORF was amplified to construct an expression vector, pGEX-4T-1-MFS40, to express the protein in Escherichia coli BL21. The gene conferred glyphosate tolerance to E. coli ER2799 cells.
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| doi_str_mv | 10.1016/j.pestbp.2017.05.013 |
| format | Article |
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[Display omitted]</description><subject>Antifungal Agents - pharmacology</subject><subject>Aspergillus oryzae - drug effects</subject><subject>Aspergillus oryzae - genetics</subject><subject>Aspergillus oryzae - metabolism</subject><subject>Aspergillus oryzae RIB40</subject><subject>cDNA library</subject><subject>Drug Resistance, Fungal - genetics</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gene Library</subject><subject>Gene screening</subject><subject>Genes, Fungal</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - pharmacology</subject><subject>Glyphosate</subject><subject>Glyphosate resistance</subject><subject>MFS40</subject><subject>SDS-PAGE</subject><issn>0048-3575</issn><issn>1095-9939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A5EcvbRO2qRpLoL4DaIXPYdsOlmzdNuatMr-e7OsevQ0DPO8M8lDyCmDnAGrLpb5gHGcD3kBTOYgcmDlDpkxUCJTqlS7ZAbA66wUUhyQwxiXAKA4qH1yUNRSiEpVM4KPDXajd96a0fcdNV1D3dTZTWPa1Jp2HX2kvaOGdvhFF-16eO-jGZEGTJPRdBbpAjukLvSrRKX4YorU3jxf0dbPgwnrY7LnTBvx5Kcekbe729frh-zp5f7x-uops2VVjJlFI0VTG8mtNQaEgIZXfM6lA7BCFGnQVFhY64wsmJSuMiWCTLgsHC-r8oicb_cOof-Ykh698tFi25oO-ylqpgpeK2C1SCjfojb0MQZ0egh-ld6qGeiNYL3UW8F6I1iD0Elwip39XJjmK2z-Qr9GE3C5BTD989Nj0NF6TI4aH9COuun9_xe-Af5dj2w</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Tao, Bo</creator><creator>Shao, Bai-Hui</creator><creator>Qiao, Yu-Xin</creator><creator>Wang, Xiao-Qin</creator><creator>Chang, Shu-Jun</creator><creator>Qiu, Li-Juan</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201708</creationdate><title>Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library</title><author>Tao, Bo ; Shao, Bai-Hui ; Qiao, Yu-Xin ; Wang, Xiao-Qin ; Chang, Shu-Jun ; Qiu, Li-Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-cea75d8a74ccaa0550d464b47f00c552a74d6e2ccfa72177f6a3e0774c72f4363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antifungal Agents - pharmacology</topic><topic>Aspergillus oryzae - drug effects</topic><topic>Aspergillus oryzae - genetics</topic><topic>Aspergillus oryzae - metabolism</topic><topic>Aspergillus oryzae RIB40</topic><topic>cDNA library</topic><topic>Drug Resistance, Fungal - genetics</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gene Library</topic><topic>Gene screening</topic><topic>Genes, Fungal</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - pharmacology</topic><topic>Glyphosate</topic><topic>Glyphosate resistance</topic><topic>MFS40</topic><topic>SDS-PAGE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, Bo</creatorcontrib><creatorcontrib>Shao, Bai-Hui</creatorcontrib><creatorcontrib>Qiao, Yu-Xin</creatorcontrib><creatorcontrib>Wang, Xiao-Qin</creatorcontrib><creatorcontrib>Chang, Shu-Jun</creatorcontrib><creatorcontrib>Qiu, Li-Juan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pesticide biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, Bo</au><au>Shao, Bai-Hui</au><au>Qiao, Yu-Xin</au><au>Wang, Xiao-Qin</au><au>Chang, Shu-Jun</au><au>Qiu, Li-Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library</atitle><jtitle>Pesticide biochemistry and physiology</jtitle><addtitle>Pestic Biochem Physiol</addtitle><date>2017-08</date><risdate>2017</risdate><volume>140</volume><spage>65</spage><epage>68</epage><pages>65-68</pages><issn>0048-3575</issn><eissn>1095-9939</eissn><abstract>Glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. If glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. While several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is interest in identifying additional genes for glyphosate tolerance. This research constructed a high-quality cDNA library form the glyphosate-resistant fungus Aspergillus oryzae RIB40 to identify genes that may confer resistance to glyphosate. Using a medium containing glyphosate (120mM), we screened several clones from the library. Based on a nucleotide sequence analysis, we identified a gene of unknown function (GenBank accession number: XM_001826835.2) that encoded a hypothetical 344-amino acid protein. The gene was named MFS40. Its ORF was amplified to construct an expression vector, pGEX-4T-1-MFS40, to express the protein in Escherichia coli BL21. The gene conferred glyphosate tolerance to E. coli ER2799 cells.
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| subjects | Antifungal Agents - pharmacology Aspergillus oryzae - drug effects Aspergillus oryzae - genetics Aspergillus oryzae - metabolism Aspergillus oryzae RIB40 cDNA library Drug Resistance, Fungal - genetics Escherichia coli - drug effects Escherichia coli - genetics Gene Expression Regulation, Fungal Gene Library Gene screening Genes, Fungal Glycine - analogs & derivatives Glycine - pharmacology Glyphosate Glyphosate resistance MFS40 SDS-PAGE |
| title | Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library |
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