Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme

A novel enantioselective N-carbobenzyloxy (Cbz)-cleaving enzyme was purified to homogeneity from Sphingomonas paucimobilis SC 16113. The gene encoding the protein has been identified and its complete DNA sequence was determined. The Cbz-cleaving enzyme was expressed in Escherichia coli.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Enzyme and microbial technology 2004-03, Vol.34 (3), p.304-312
Hauptverfasser:	Nanduri, Venkata B, Goldberg, Steven, Johnston, Robert, Patel, Ramesh N
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cloning Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Heterologus expression Methods. Procedures. Technologies N-Carbobenzyloxy-cleaving enzyme Sphingomonas paucimobilis Vectors (cloning, transfer, expression). Insertion sequences and transposons
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A novel enantioselective N-carbobenzyloxy (Cbz)-cleaving enzyme was purified to homogeneity from Sphingomonas paucimobilis SC 16113. The gene encoding the protein has been identified and its complete DNA sequence was determined. The Cbz-cleaving enzyme was expressed in Escherichia coli.
ISSN:	0141-0229 1879-0909
DOI:	10.1016/j.enzmictec.2003.11.012