Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors
•Kiss2 increased CRE-luc activity in COS-7 cells expressing its cognate receptor.•Kiss2 stimulated SRE-luc activity in COS-7 cells transfected with its cognate receptor.•These stimulatory effects were reduced by inhibitors of PKA and PKC pathways, respectively.•LPXRFa-2 inhibited Kiss2-induced CRE-l...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2017-09, Vol.95, p.62-67 |
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| creator | Wang, Bin Yang, Guokun Liu, Quan Qin, Jingkai Xu, Yongjiang Li, Wensheng Liu, Xuezhou Shi, Bao |
| description | •Kiss2 increased CRE-luc activity in COS-7 cells expressing its cognate receptor.•Kiss2 stimulated SRE-luc activity in COS-7 cells transfected with its cognate receptor.•These stimulatory effects were reduced by inhibitors of PKA and PKC pathways, respectively.•LPXRFa-2 inhibited Kiss2-induced CRE-luc activity in a dose-dependent manner.
Kisspeptin (Kiss) acts as a positive regulator of reproduction by acting on gonadotropes and gonadotropin-releasing hormone (GnRH) neurons. Despite its functional significance, the intricate web of intracellular signal transduction pathways in response to Kiss is still far from being fully understood in teleosts. Accordingly, we investigated the molecular mechanism of Kiss action and its possible interaction with LPXRFa signaling in this study. In vitro functional analysis revealed that synthetic tongue sole Kiss2 decapeptide increased the cAMP responsive element-dependent luciferase (CRE-luc) activity in COS-7 cells transfected with its cognate receptor, while this stimulatory effect was markedly reduced by two inhibitors of the adenylate cyclase (AC)/protein kinase A (PKA) pathway. Similarly, Kiss2 also significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity, whereas this stimulatory effect was evidently attenuated by two inhibitors of the phospholipase C (PLC)/protein kinase C (PKC) pathway. In addition, LPXRFa-2 suppressed Kiss2-elicited CRE-luc activity in a dose-dependent manner. Taken together, Kiss2 utilizes both AC/PKA and PLC/PKC pathways to exert its functions via its cognate receptor and LPXRFa may antagonize the action of Kiss2 by inhibiting kisspeptin signaling. As far as we know, this study is the first to characterize the half-smooth tongue sole kisspeptin and LPXRFa signaling pathway in COS-7 cells transfected with their cognate receptors and provides novel information on the interaction between LPXRFa system and kisspeptin system in teleosts. |
| doi_str_mv | 10.1016/j.peptides.2017.07.014 |
| format | Article |
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Kisspeptin (Kiss) acts as a positive regulator of reproduction by acting on gonadotropes and gonadotropin-releasing hormone (GnRH) neurons. Despite its functional significance, the intricate web of intracellular signal transduction pathways in response to Kiss is still far from being fully understood in teleosts. Accordingly, we investigated the molecular mechanism of Kiss action and its possible interaction with LPXRFa signaling in this study. In vitro functional analysis revealed that synthetic tongue sole Kiss2 decapeptide increased the cAMP responsive element-dependent luciferase (CRE-luc) activity in COS-7 cells transfected with its cognate receptor, while this stimulatory effect was markedly reduced by two inhibitors of the adenylate cyclase (AC)/protein kinase A (PKA) pathway. Similarly, Kiss2 also significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity, whereas this stimulatory effect was evidently attenuated by two inhibitors of the phospholipase C (PLC)/protein kinase C (PKC) pathway. In addition, LPXRFa-2 suppressed Kiss2-elicited CRE-luc activity in a dose-dependent manner. Taken together, Kiss2 utilizes both AC/PKA and PLC/PKC pathways to exert its functions via its cognate receptor and LPXRFa may antagonize the action of Kiss2 by inhibiting kisspeptin signaling. As far as we know, this study is the first to characterize the half-smooth tongue sole kisspeptin and LPXRFa signaling pathway in COS-7 cells transfected with their cognate receptors and provides novel information on the interaction between LPXRFa system and kisspeptin system in teleosts.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2017.07.014</identifier><identifier>PMID: 28754347</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenylyl Cyclases - genetics ; Adenylyl Cyclases - metabolism ; Animals ; Cercopithecus aethiops ; COS Cells ; Cyclic AMP-Dependent Protein Kinases - genetics ; Cynoglossus semilaevis ; Fishes - genetics ; Gene Expression Regulation - genetics ; GnIH ; Gonadotrophs - chemistry ; Gonadotrophs - metabolism ; Gonadotropin-Releasing Hormone - chemistry ; Gonadotropin-Releasing Hormone - genetics ; Gonadotropins - genetics ; Gonadotropins - metabolism ; Kisspeptin ; Kisspeptins - genetics ; Kisspeptins - metabolism ; LPXRFa ; Neurons - metabolism ; Neurons - physiology ; Protein Kinase C - genetics ; Protein Kinase C - metabolism ; Reproduction - genetics ; Reproduction - physiology ; Signal Transduction - genetics ; Signaling pathway ; Transfection ; Type C Phospholipases - genetics ; Type C Phospholipases - metabolism</subject><ispartof>Peptides (New York, N.Y. : 1980), 2017-09, Vol.95, p.62-67</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-4e7ac86a82d183b99f19846d3c4d434e83eacaf2b94c495cb86b01d6447ccf3</citedby><cites>FETCH-LOGICAL-c368t-4e7ac86a82d183b99f19846d3c4d434e83eacaf2b94c495cb86b01d6447ccf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0196978117302498$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28754347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Yang, Guokun</creatorcontrib><creatorcontrib>Liu, Quan</creatorcontrib><creatorcontrib>Qin, Jingkai</creatorcontrib><creatorcontrib>Xu, Yongjiang</creatorcontrib><creatorcontrib>Li, Wensheng</creatorcontrib><creatorcontrib>Liu, Xuezhou</creatorcontrib><creatorcontrib>Shi, Bao</creatorcontrib><title>Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>•Kiss2 increased CRE-luc activity in COS-7 cells expressing its cognate receptor.•Kiss2 stimulated SRE-luc activity in COS-7 cells transfected with its cognate receptor.•These stimulatory effects were reduced by inhibitors of PKA and PKC pathways, respectively.•LPXRFa-2 inhibited Kiss2-induced CRE-luc activity in a dose-dependent manner.
Kisspeptin (Kiss) acts as a positive regulator of reproduction by acting on gonadotropes and gonadotropin-releasing hormone (GnRH) neurons. Despite its functional significance, the intricate web of intracellular signal transduction pathways in response to Kiss is still far from being fully understood in teleosts. Accordingly, we investigated the molecular mechanism of Kiss action and its possible interaction with LPXRFa signaling in this study. In vitro functional analysis revealed that synthetic tongue sole Kiss2 decapeptide increased the cAMP responsive element-dependent luciferase (CRE-luc) activity in COS-7 cells transfected with its cognate receptor, while this stimulatory effect was markedly reduced by two inhibitors of the adenylate cyclase (AC)/protein kinase A (PKA) pathway. Similarly, Kiss2 also significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity, whereas this stimulatory effect was evidently attenuated by two inhibitors of the phospholipase C (PLC)/protein kinase C (PKC) pathway. In addition, LPXRFa-2 suppressed Kiss2-elicited CRE-luc activity in a dose-dependent manner. Taken together, Kiss2 utilizes both AC/PKA and PLC/PKC pathways to exert its functions via its cognate receptor and LPXRFa may antagonize the action of Kiss2 by inhibiting kisspeptin signaling. As far as we know, this study is the first to characterize the half-smooth tongue sole kisspeptin and LPXRFa signaling pathway in COS-7 cells transfected with their cognate receptors and provides novel information on the interaction between LPXRFa system and kisspeptin system in teleosts.</description><subject>Adenylyl Cyclases - genetics</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Animals</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Cyclic AMP-Dependent Protein Kinases - genetics</subject><subject>Cynoglossus semilaevis</subject><subject>Fishes - genetics</subject><subject>Gene Expression Regulation - genetics</subject><subject>GnIH</subject><subject>Gonadotrophs - chemistry</subject><subject>Gonadotrophs - metabolism</subject><subject>Gonadotropin-Releasing Hormone - chemistry</subject><subject>Gonadotropin-Releasing Hormone - genetics</subject><subject>Gonadotropins - genetics</subject><subject>Gonadotropins - metabolism</subject><subject>Kisspeptin</subject><subject>Kisspeptins - genetics</subject><subject>Kisspeptins - metabolism</subject><subject>LPXRFa</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Protein Kinase C - genetics</subject><subject>Protein Kinase C - metabolism</subject><subject>Reproduction - genetics</subject><subject>Reproduction - physiology</subject><subject>Signal Transduction - genetics</subject><subject>Signaling pathway</subject><subject>Transfection</subject><subject>Type C Phospholipases - genetics</subject><subject>Type C Phospholipases - metabolism</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURS0EokPhFyovWTSDnXgSewcaUVpppCLKgp3l2C-Jh4wdbIdq_pMPqsO0IFZIT_Lm3Pv87kXogpI1JbR-t19PMCVrIK5LQps1yUPZM7SivKmKDa3Fc7QiVNSFaDg9Q69i3BNCGBP8JTorebNhFWtW6NeNG2xrkw9HrHSy3mHf4eRdPwOOfgS8-_zty5W6xGkAPNmorQPsQxr86PuF7b1TxqfgJ-sK-9dt8OHgHVzibLloo-2dGq3r8aTScK-O2DozazC4Pf6z8LuN8fdxLhN4e3tXNFjDOEacgnKxA52y6N6mYfG1AWufnRPgADrLfIiv0YtOjRHePL7n6O7q49ftdbG7_XSz_bArdFXzVDBolOa14qWhvGqF6KjgrDaVZianA7wCpVVXtoJpJja65XVLqKkZa7TuqnP09uQ6Bf9jhpjkIceTP6oc-DlKKkq2EYJXTUbrE6qDjzFAJ6dgDyocJSVy6VPu5VOfculTkjyUZeHF4465PYD5I3sqMAPvTwDkO39aCDI3BC7HanMcSRpv_7fjAeF4u1Q</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Wang, Bin</creator><creator>Yang, Guokun</creator><creator>Liu, Quan</creator><creator>Qin, Jingkai</creator><creator>Xu, Yongjiang</creator><creator>Li, Wensheng</creator><creator>Liu, Xuezhou</creator><creator>Shi, Bao</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors</title><author>Wang, Bin ; Yang, Guokun ; Liu, Quan ; Qin, Jingkai ; Xu, Yongjiang ; Li, Wensheng ; Liu, Xuezhou ; Shi, Bao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-4e7ac86a82d183b99f19846d3c4d434e83eacaf2b94c495cb86b01d6447ccf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenylyl Cyclases - genetics</topic><topic>Adenylyl Cyclases - metabolism</topic><topic>Animals</topic><topic>Cercopithecus aethiops</topic><topic>COS Cells</topic><topic>Cyclic AMP-Dependent Protein Kinases - genetics</topic><topic>Cynoglossus semilaevis</topic><topic>Fishes - genetics</topic><topic>Gene Expression Regulation - genetics</topic><topic>GnIH</topic><topic>Gonadotrophs - chemistry</topic><topic>Gonadotrophs - metabolism</topic><topic>Gonadotropin-Releasing Hormone - chemistry</topic><topic>Gonadotropin-Releasing Hormone - genetics</topic><topic>Gonadotropins - genetics</topic><topic>Gonadotropins - metabolism</topic><topic>Kisspeptin</topic><topic>Kisspeptins - genetics</topic><topic>Kisspeptins - metabolism</topic><topic>LPXRFa</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Protein Kinase C - genetics</topic><topic>Protein Kinase C - metabolism</topic><topic>Reproduction - genetics</topic><topic>Reproduction - physiology</topic><topic>Signal Transduction - genetics</topic><topic>Signaling pathway</topic><topic>Transfection</topic><topic>Type C Phospholipases - genetics</topic><topic>Type C Phospholipases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Yang, Guokun</creatorcontrib><creatorcontrib>Liu, Quan</creatorcontrib><creatorcontrib>Qin, Jingkai</creatorcontrib><creatorcontrib>Xu, Yongjiang</creatorcontrib><creatorcontrib>Li, Wensheng</creatorcontrib><creatorcontrib>Liu, Xuezhou</creatorcontrib><creatorcontrib>Shi, Bao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Bin</au><au>Yang, Guokun</au><au>Liu, Quan</au><au>Qin, Jingkai</au><au>Xu, Yongjiang</au><au>Li, Wensheng</au><au>Liu, Xuezhou</au><au>Shi, Bao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2017-09</date><risdate>2017</risdate><volume>95</volume><spage>62</spage><epage>67</epage><pages>62-67</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><abstract>•Kiss2 increased CRE-luc activity in COS-7 cells expressing its cognate receptor.•Kiss2 stimulated SRE-luc activity in COS-7 cells transfected with its cognate receptor.•These stimulatory effects were reduced by inhibitors of PKA and PKC pathways, respectively.•LPXRFa-2 inhibited Kiss2-induced CRE-luc activity in a dose-dependent manner.
Kisspeptin (Kiss) acts as a positive regulator of reproduction by acting on gonadotropes and gonadotropin-releasing hormone (GnRH) neurons. Despite its functional significance, the intricate web of intracellular signal transduction pathways in response to Kiss is still far from being fully understood in teleosts. Accordingly, we investigated the molecular mechanism of Kiss action and its possible interaction with LPXRFa signaling in this study. In vitro functional analysis revealed that synthetic tongue sole Kiss2 decapeptide increased the cAMP responsive element-dependent luciferase (CRE-luc) activity in COS-7 cells transfected with its cognate receptor, while this stimulatory effect was markedly reduced by two inhibitors of the adenylate cyclase (AC)/protein kinase A (PKA) pathway. Similarly, Kiss2 also significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity, whereas this stimulatory effect was evidently attenuated by two inhibitors of the phospholipase C (PLC)/protein kinase C (PKC) pathway. In addition, LPXRFa-2 suppressed Kiss2-elicited CRE-luc activity in a dose-dependent manner. Taken together, Kiss2 utilizes both AC/PKA and PLC/PKC pathways to exert its functions via its cognate receptor and LPXRFa may antagonize the action of Kiss2 by inhibiting kisspeptin signaling. As far as we know, this study is the first to characterize the half-smooth tongue sole kisspeptin and LPXRFa signaling pathway in COS-7 cells transfected with their cognate receptors and provides novel information on the interaction between LPXRFa system and kisspeptin system in teleosts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28754347</pmid><doi>10.1016/j.peptides.2017.07.014</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-9781 |
| ispartof | Peptides (New York, N.Y. : 1980), 2017-09, Vol.95, p.62-67 |
| issn | 0196-9781 1873-5169 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenylyl Cyclases - genetics Adenylyl Cyclases - metabolism Animals Cercopithecus aethiops COS Cells Cyclic AMP-Dependent Protein Kinases - genetics Cynoglossus semilaevis Fishes - genetics Gene Expression Regulation - genetics GnIH Gonadotrophs - chemistry Gonadotrophs - metabolism Gonadotropin-Releasing Hormone - chemistry Gonadotropin-Releasing Hormone - genetics Gonadotropins - genetics Gonadotropins - metabolism Kisspeptin Kisspeptins - genetics Kisspeptins - metabolism LPXRFa Neurons - metabolism Neurons - physiology Protein Kinase C - genetics Protein Kinase C - metabolism Reproduction - genetics Reproduction - physiology Signal Transduction - genetics Signaling pathway Transfection Type C Phospholipases - genetics Type C Phospholipases - metabolism |
| title | Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors |
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