Common carp metallothionein-1 gene: cDNA cloning, gene structure and expression studies

Metallothionein-1 (MT-1) cDNA clones were isolated from a common carp ( Cyprinus carpio) uninduced hepatopancreas cDNA library. Northern blot assay using the common carp (cc) MT-1 cDNA as a probe showed high fold induction of ccMT mRNA levels in the intestine and kidney following exposure to Cd 2+ a...

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Veröffentlicht in:	Biochimica et biophysica acta 2004-01, Vol.1676 (2), p.162-171
Hauptverfasser:	Chan, Pat Chun, Shiu, Carmen K.M., Wong, Felice W.-Y., Wong, Judy Ka-Yee, Lam, Kwok Lim, Chan, King Ming
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Biomarker Blotting, Northern Blotting, Southern Carps - genetics Cloning, Molecular Cyprinus carpio DNA Primers DNA, Complementary - biosynthesis DNA, Complementary - genetics DNA, Complementary - isolation & purification Gene promoter analysis and reporter gene system Gene regulation Hepatocytes - enzymology Hepatopancreas - enzymology Metal toxicity Metallothionein - biosynthesis Metallothionein - genetics Metallothionein - isolation & purification metallothionen Molecular Sequence Data PCR-cloning Polymerase Chain Reaction Primary culture of hepatocyte Promoter Regions, Genetic RNA, Messenger - analysis RNA, Messenger - biosynthesis Stress
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