Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples

Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples

Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction‐based detection system and a culture method using SM‐ID agar as the selective plating medium. The BAX method ide...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of food science 2003-04, Vol.68 (3), p.992-995, Article 992
Hauptverfasser: Wu, Chih-Chuan, Liu, Cheng-I, Tsai, Chin-En, Yeh, Kuang-Sheng
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Food contamination & poisoning
Food science
polymerase chain reaction
Pork
pork carcass
Salmonella
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 995
container_issue 3
container_start_page 992
container_title Journal of food science
container_volume 68
creator Wu, Chih-Chuan
Liu, Cheng-I
Tsai, Chin-En
Yeh, Kuang-Sheng
description Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction‐based detection system and a culture method using SM‐ID agar as the selective plating medium. The BAX method identified 14 samples as positive for Salmonella. The 8 samples identified as Salmonella‐positive by the SM‐ID method were also BAX‐positive. Inoculation studies showed BAX detected Salmonella in samples having initial 1.4 × 101 cfu/mL Salmonella inoculum prior to the enrichment process in the presence of 3.0 × 106 cfu/mL of non‐Salmonella florae. BAX provides a rapid screening alternative to the culture method for Salmonella detection.
doi_str_mv 10.1111/j.1365-2621.2003.tb08276.x
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19233229</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>782025051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4132-8d4cb56cd0cc983dcf5402ac2280079dcbc275314fa41f82ce9dd9dd00fe198b3</originalsourceid><addsrcrecordid>eNqVkUtvEzEUha0KJELKf7C66G4GP-bh6QZo-qBVVR6hgNhYjue6OPWMgz2hyb-vh1QsugLLkmWfcz9f-yB0QElO03i9zCmvyoxVjOaMEJ4PCyJYXeWbPTShdUkyLgr6DE0IYSyjtKhfoJcxLsm459UEbU5_K7dWg_U99gYr_NG7bQdBRcCzn8r2-DMoPcrZcTpr8XwbB-iw8QGfwABJ6m_xXLnO9-CcwvMVaAsRm-C7BAt3eKaCVjEmxfe3kLzdykHcR8-NchFePa5TdHN2-mX2Prv6cH4xe3eV6YJylom20Iuy0i3RuhG81aYsCFOaMUFI3bR6oVldcloYVVAjmIambdMkxABtxIJP0eGOuwr-1xriIDsb9dhqD34dJW0Y54w1yXjwxLj069Cn3pKn4KKiXCTT0c6kg48xgJGrYDsVtpISOSYil3JMRI6JyDER-ZiI3KTit0-KtR3-fP0QlHX_hnizQ9xbB9v_uFxenp3Mm_TWKcp2BJty3PwlqHAnq5rXpfx2fS75Mfsq2I_v8hN_AAfxuCw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>194386138</pqid></control><display><type>article</type><title>Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples</title><source>Wiley-Blackwell Journals</source><creator>Wu, Chih-Chuan ; Liu, Cheng-I ; Tsai, Chin-En ; Yeh, Kuang-Sheng</creator><creatorcontrib>Wu, Chih-Chuan ; Liu, Cheng-I ; Tsai, Chin-En ; Yeh, Kuang-Sheng</creatorcontrib><description>Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction‐based detection system and a culture method using SM‐ID agar as the selective plating medium. The BAX method identified 14 samples as positive for Salmonella. The 8 samples identified as Salmonella‐positive by the SM‐ID method were also BAX‐positive. Inoculation studies showed BAX detected Salmonella in samples having initial 1.4 × 101 cfu/mL Salmonella inoculum prior to the enrichment process in the presence of 3.0 × 106 cfu/mL of non‐Salmonella florae. BAX provides a rapid screening alternative to the culture method for Salmonella detection.</description><identifier>ISSN: 0022-1147</identifier><identifier>EISSN: 1750-3841</identifier><identifier>DOI: 10.1111/j.1365-2621.2003.tb08276.x</identifier><identifier>CODEN: JFDSAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteria ; Food contamination &amp; poisoning ; Food science ; polymerase chain reaction ; Pork ; pork carcass ; Salmonella</subject><ispartof>Journal of food science, 2003-04, Vol.68 (3), p.992-995, Article 992</ispartof><rights>Copyright Institute of Food Technologists Apr 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4132-8d4cb56cd0cc983dcf5402ac2280079dcbc275314fa41f82ce9dd9dd00fe198b3</citedby><cites>FETCH-LOGICAL-c4132-8d4cb56cd0cc983dcf5402ac2280079dcbc275314fa41f82ce9dd9dd00fe198b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2621.2003.tb08276.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2621.2003.tb08276.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids></links><search><creatorcontrib>Wu, Chih-Chuan</creatorcontrib><creatorcontrib>Liu, Cheng-I</creatorcontrib><creatorcontrib>Tsai, Chin-En</creatorcontrib><creatorcontrib>Yeh, Kuang-Sheng</creatorcontrib><title>Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples</title><title>Journal of food science</title><description>Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction‐based detection system and a culture method using SM‐ID agar as the selective plating medium. The BAX method identified 14 samples as positive for Salmonella. The 8 samples identified as Salmonella‐positive by the SM‐ID method were also BAX‐positive. Inoculation studies showed BAX detected Salmonella in samples having initial 1.4 × 101 cfu/mL Salmonella inoculum prior to the enrichment process in the presence of 3.0 × 106 cfu/mL of non‐Salmonella florae. BAX provides a rapid screening alternative to the culture method for Salmonella detection.</description><subject>Bacteria</subject><subject>Food contamination &amp; poisoning</subject><subject>Food science</subject><subject>polymerase chain reaction</subject><subject>Pork</subject><subject>pork carcass</subject><subject>Salmonella</subject><issn>0022-1147</issn><issn>1750-3841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqVkUtvEzEUha0KJELKf7C66G4GP-bh6QZo-qBVVR6hgNhYjue6OPWMgz2hyb-vh1QsugLLkmWfcz9f-yB0QElO03i9zCmvyoxVjOaMEJ4PCyJYXeWbPTShdUkyLgr6DE0IYSyjtKhfoJcxLsm459UEbU5_K7dWg_U99gYr_NG7bQdBRcCzn8r2-DMoPcrZcTpr8XwbB-iw8QGfwABJ6m_xXLnO9-CcwvMVaAsRm-C7BAt3eKaCVjEmxfe3kLzdykHcR8-NchFePa5TdHN2-mX2Prv6cH4xe3eV6YJylom20Iuy0i3RuhG81aYsCFOaMUFI3bR6oVldcloYVVAjmIambdMkxABtxIJP0eGOuwr-1xriIDsb9dhqD34dJW0Y54w1yXjwxLj069Cn3pKn4KKiXCTT0c6kg48xgJGrYDsVtpISOSYil3JMRI6JyDER-ZiI3KTit0-KtR3-fP0QlHX_hnizQ9xbB9v_uFxenp3Mm_TWKcp2BJty3PwlqHAnq5rXpfx2fS75Mfsq2I_v8hN_AAfxuCw</recordid><startdate>200304</startdate><enddate>200304</enddate><creator>Wu, Chih-Chuan</creator><creator>Liu, Cheng-I</creator><creator>Tsai, Chin-En</creator><creator>Yeh, Kuang-Sheng</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QR</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>200304</creationdate><title>Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples</title><author>Wu, Chih-Chuan ; Liu, Cheng-I ; Tsai, Chin-En ; Yeh, Kuang-Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4132-8d4cb56cd0cc983dcf5402ac2280079dcbc275314fa41f82ce9dd9dd00fe198b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacteria</topic><topic>Food contamination &amp; poisoning</topic><topic>Food science</topic><topic>polymerase chain reaction</topic><topic>Pork</topic><topic>pork carcass</topic><topic>Salmonella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Chih-Chuan</creatorcontrib><creatorcontrib>Liu, Cheng-I</creatorcontrib><creatorcontrib>Tsai, Chin-En</creatorcontrib><creatorcontrib>Yeh, Kuang-Sheng</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of food science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Chih-Chuan</au><au>Liu, Cheng-I</au><au>Tsai, Chin-En</au><au>Yeh, Kuang-Sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples</atitle><jtitle>Journal of food science</jtitle><date>2003-04</date><risdate>2003</risdate><volume>68</volume><issue>3</issue><spage>992</spage><epage>995</epage><pages>992-995</pages><artnum>992</artnum><issn>0022-1147</issn><eissn>1750-3841</eissn><coden>JFDSAZ</coden><abstract>Pork carcass sponge samples (n = 230) collected from 10 Taiwanese slaughter plants were screened for Salmonella using 2 methods: BAX® for Screening/Salmonella, a polymerase chain reaction‐based detection system and a culture method using SM‐ID agar as the selective plating medium. The BAX method identified 14 samples as positive for Salmonella. The 8 samples identified as Salmonella‐positive by the SM‐ID method were also BAX‐positive. Inoculation studies showed BAX detected Salmonella in samples having initial 1.4 × 101 cfu/mL Salmonella inoculum prior to the enrichment process in the presence of 3.0 × 106 cfu/mL of non‐Salmonella florae. BAX provides a rapid screening alternative to the culture method for Salmonella detection.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1365-2621.2003.tb08276.x</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-1147
ispartof Journal of food science, 2003-04, Vol.68 (3), p.992-995, Article 992
issn 0022-1147
1750-3841
language eng
recordid cdi_proquest_miscellaneous_19233229
source Wiley-Blackwell Journals
subjects Bacteria
Food contamination & poisoning
Food science
polymerase chain reaction
Pork
pork carcass
Salmonella
title Evaluation of a Polymerase Chain Reaction-Based System for Detecting Salmonella Species from Pork Carcass Sponge Samples
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T14%3A35%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20a%20Polymerase%20Chain%20Reaction-Based%20System%20for%20Detecting%20Salmonella%20Species%20from%20Pork%20Carcass%20Sponge%20Samples&rft.jtitle=Journal%20of%20food%20science&rft.au=Wu,%20Chih-Chuan&rft.date=2003-04&rft.volume=68&rft.issue=3&rft.spage=992&rft.epage=995&rft.pages=992-995&rft.artnum=992&rft.issn=0022-1147&rft.eissn=1750-3841&rft.coden=JFDSAZ&rft_id=info:doi/10.1111/j.1365-2621.2003.tb08276.x&rft_dat=%3Cproquest_cross%3E782025051%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=194386138&rft_id=info:pmid/&rfr_iscdi=true