Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty
To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP). Prospective, interventional, consecutive case series. This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years)...
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| Veröffentlicht in: | American journal of ophthalmology 2017-09, Vol.181, p.166-173 |
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| creator | Yagi-Yaguchi, Yukari Yamaguchi, Takefumi Higa, Kazunari Suzuki, Terumasa Yazu, Hiroyuki Aketa, Naohiko Satake, Yoshiyuki Tsubota, Kazuo Shimazaki, Jun |
| description | To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP).
Prospective, interventional, consecutive case series.
This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes).
The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P = .02) and IFN-γ (r = −0.412, P = .029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P = .024), MCP-1 (r = −0.444, P = .011), and IFN-γ (r = −0.474, P = .006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P < .05).
Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP. |
| doi_str_mv | 10.1016/j.ajo.2017.07.005 |
| format | Article |
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Prospective, interventional, consecutive case series.
This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes).
The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P = .02) and IFN-γ (r = −0.412, P = .029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P = .024), MCP-1 (r = −0.444, P = .011), and IFN-γ (r = −0.474, P = .006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P < .05).
Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.</description><identifier>ISSN: 0002-9394</identifier><identifier>EISSN: 1879-1891</identifier><identifier>DOI: 10.1016/j.ajo.2017.07.005</identifier><identifier>PMID: 28734818</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aged ; Aged, 80 and over ; Apoptosis ; Aqueous Humor - metabolism ; Cataracts ; Cell Count ; Cornea ; Corneal Endothelial Cell Loss - diagnosis ; Corneal Endothelial Cell Loss - etiology ; Corneal Endothelial Cell Loss - metabolism ; Cytokines ; Cytokines - metabolism ; Endothelium ; Endothelium, Corneal - pathology ; Eye surgery ; Female ; Humans ; Immunoassay ; Keratoplasty, Penetrating ; Male ; Microscopy ; Middle Aged ; Ophthalmology ; Postoperative Complications ; Postoperative Period ; Preoperative Period ; Prospective Studies ; Proteins ; Transplants & implants ; Tumor necrosis factor-TNF</subject><ispartof>American journal of ophthalmology, 2017-09, Vol.181, p.166-173</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier Limited Sep 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-585b09e31f19f9ff4282969dc7ea65ba9f26d02df44ff05809d356b30303f73</citedby><cites>FETCH-LOGICAL-c381t-585b09e31f19f9ff4282969dc7ea65ba9f26d02df44ff05809d356b30303f73</cites><orcidid>0000-0002-7371-6558 ; 0000-0002-2546-2813 ; 0000-0002-7980-7741 ; 0000-0002-2357-8265</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ajo.2017.07.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28734818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yagi-Yaguchi, Yukari</creatorcontrib><creatorcontrib>Yamaguchi, Takefumi</creatorcontrib><creatorcontrib>Higa, Kazunari</creatorcontrib><creatorcontrib>Suzuki, Terumasa</creatorcontrib><creatorcontrib>Yazu, Hiroyuki</creatorcontrib><creatorcontrib>Aketa, Naohiko</creatorcontrib><creatorcontrib>Satake, Yoshiyuki</creatorcontrib><creatorcontrib>Tsubota, Kazuo</creatorcontrib><creatorcontrib>Shimazaki, Jun</creatorcontrib><title>Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty</title><title>American journal of ophthalmology</title><addtitle>Am J Ophthalmol</addtitle><description>To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP).
Prospective, interventional, consecutive case series.
This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes).
The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P = .02) and IFN-γ (r = −0.412, P = .029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P = .024), MCP-1 (r = −0.444, P = .011), and IFN-γ (r = −0.474, P = .006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P < .05).
Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis</subject><subject>Aqueous Humor - metabolism</subject><subject>Cataracts</subject><subject>Cell Count</subject><subject>Cornea</subject><subject>Corneal Endothelial Cell Loss - diagnosis</subject><subject>Corneal Endothelial Cell Loss - etiology</subject><subject>Corneal Endothelial Cell Loss - metabolism</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Endothelium</subject><subject>Endothelium, Corneal - pathology</subject><subject>Eye surgery</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Keratoplasty, Penetrating</subject><subject>Male</subject><subject>Microscopy</subject><subject>Middle Aged</subject><subject>Ophthalmology</subject><subject>Postoperative Complications</subject><subject>Postoperative Period</subject><subject>Preoperative Period</subject><subject>Prospective Studies</subject><subject>Proteins</subject><subject>Transplants & implants</subject><subject>Tumor necrosis factor-TNF</subject><issn>0002-9394</issn><issn>1879-1891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-L1DAYxoMo7uzqB_AiAS976Zg0TZvgaRjWVRxwWQWPIZO8cVM7TU3Sgbn42U2Z1YMHyQsh5Pc-758HoVeUrCmh7dt-rfuwrgnt1qQE4U_QiopOVlRI-hStCCF1JZlsLtBlSn15tl3TPUcXtehYI6hYoV93EcIEUWd_BLz5OUOYE96ecvjhR8A7OMKQ8CaWv5SC8TqDxd98fsAa3-vJW3wPdjbZhxH7Ed-MNuQHGLwe8BaGJdVliPgORshLkfE7_rRUC9OgUz69QM-cHhK8fLyv0Jf3N1-3H6rd59uP282uMkzQXHHB90QCo45KJ51ralHLVlrTgW75XktXt5bU1jWNc4QLIi3j7Z6RclzHrtD1WXWKoUyYsjr4ZEp7elzGVVTWjBIpeVPQN_-gfZjjWHorFOOsK1trC0XPlIkhpQhOTdEfdDwpStRijepVsUYt1ihSgvCS8_pRed4fwP7N-ONFAd6dgbJxOHqIKhkPowHrI5isbPD_kf8NKxefUA</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Yagi-Yaguchi, Yukari</creator><creator>Yamaguchi, Takefumi</creator><creator>Higa, Kazunari</creator><creator>Suzuki, Terumasa</creator><creator>Yazu, Hiroyuki</creator><creator>Aketa, Naohiko</creator><creator>Satake, Yoshiyuki</creator><creator>Tsubota, Kazuo</creator><creator>Shimazaki, Jun</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7371-6558</orcidid><orcidid>https://orcid.org/0000-0002-2546-2813</orcidid><orcidid>https://orcid.org/0000-0002-7980-7741</orcidid><orcidid>https://orcid.org/0000-0002-2357-8265</orcidid></search><sort><creationdate>201709</creationdate><title>Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty</title><author>Yagi-Yaguchi, Yukari ; Yamaguchi, Takefumi ; Higa, Kazunari ; Suzuki, Terumasa ; Yazu, Hiroyuki ; Aketa, Naohiko ; Satake, Yoshiyuki ; Tsubota, Kazuo ; Shimazaki, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-585b09e31f19f9ff4282969dc7ea65ba9f26d02df44ff05809d356b30303f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis</topic><topic>Aqueous Humor - metabolism</topic><topic>Cataracts</topic><topic>Cell Count</topic><topic>Cornea</topic><topic>Corneal Endothelial Cell Loss - diagnosis</topic><topic>Corneal Endothelial Cell Loss - etiology</topic><topic>Corneal Endothelial Cell Loss - metabolism</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Endothelium</topic><topic>Endothelium, Corneal - pathology</topic><topic>Eye surgery</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Keratoplasty, Penetrating</topic><topic>Male</topic><topic>Microscopy</topic><topic>Middle Aged</topic><topic>Ophthalmology</topic><topic>Postoperative Complications</topic><topic>Postoperative Period</topic><topic>Preoperative Period</topic><topic>Prospective Studies</topic><topic>Proteins</topic><topic>Transplants & implants</topic><topic>Tumor necrosis factor-TNF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yagi-Yaguchi, Yukari</creatorcontrib><creatorcontrib>Yamaguchi, Takefumi</creatorcontrib><creatorcontrib>Higa, Kazunari</creatorcontrib><creatorcontrib>Suzuki, Terumasa</creatorcontrib><creatorcontrib>Yazu, Hiroyuki</creatorcontrib><creatorcontrib>Aketa, Naohiko</creatorcontrib><creatorcontrib>Satake, Yoshiyuki</creatorcontrib><creatorcontrib>Tsubota, Kazuo</creatorcontrib><creatorcontrib>Shimazaki, Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yagi-Yaguchi, Yukari</au><au>Yamaguchi, Takefumi</au><au>Higa, Kazunari</au><au>Suzuki, Terumasa</au><au>Yazu, Hiroyuki</au><au>Aketa, Naohiko</au><au>Satake, Yoshiyuki</au><au>Tsubota, Kazuo</au><au>Shimazaki, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty</atitle><jtitle>American journal of ophthalmology</jtitle><addtitle>Am J Ophthalmol</addtitle><date>2017-09</date><risdate>2017</risdate><volume>181</volume><spage>166</spage><epage>173</epage><pages>166-173</pages><issn>0002-9394</issn><eissn>1879-1891</eissn><abstract>To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP).
Prospective, interventional, consecutive case series.
This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm2 at 6 months (24 eyes), and ECD < 1200 cells/mm2 at 6 months (13 eyes).
The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = −0.428, P = .02) and IFN-γ (r = −0.412, P = .029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = −0.399, P = .024), MCP-1 (r = −0.444, P = .011), and IFN-γ (r = −0.474, P = .006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm2 compared with those with ECD ≥ 1200 cells/mm2 at 6 months (P < .05).
Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28734818</pmid><doi>10.1016/j.ajo.2017.07.005</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7371-6558</orcidid><orcidid>https://orcid.org/0000-0002-2546-2813</orcidid><orcidid>https://orcid.org/0000-0002-7980-7741</orcidid><orcidid>https://orcid.org/0000-0002-2357-8265</orcidid></addata></record> |
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| subjects | Aged Aged, 80 and over Apoptosis Aqueous Humor - metabolism Cataracts Cell Count Cornea Corneal Endothelial Cell Loss - diagnosis Corneal Endothelial Cell Loss - etiology Corneal Endothelial Cell Loss - metabolism Cytokines Cytokines - metabolism Endothelium Endothelium, Corneal - pathology Eye surgery Female Humans Immunoassay Keratoplasty, Penetrating Male Microscopy Middle Aged Ophthalmology Postoperative Complications Postoperative Period Preoperative Period Prospective Studies Proteins Transplants & implants Tumor necrosis factor-TNF |
| title | Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty |
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