A new PPARγ/DNA origami biochromatography and offline high performance liquid chromatography-mass spectrometry method for screening PPARγ receptor antagonists from ginsenosides

To rapidly identify novel PPARγ ligands, a robust binding assay amenable to high-efficiency screening toward PPARγ would be desirable. In this study, a new PPARγ assembled on DNA origami (PPARγ/DNA origami) biochromatography drug screening model was constructed and evaluated. The method was used to screen active ingredients acted on PPARγ from the total ginsenosides. The total ginsenosides were handled on this biochromatography column by HPLC. The collected retention fraction from the biochromatography column was analyzed by HPLC and HPLC/MS. The results showed that ginsenoside Re from the total ginsenosides was the targeted component which could act on PPARγ receptor in similar manner of rosiglitazone as a control drug. This method will be a useful method for drug screening with natural medicinal herbs as a leading compound resource, compared with previous drug screening, this method without the need for complex and time-consuming separation steps previously. •A new PPARγ/DNA origami biochromatography-offline-HPLC/MS drug screening method was constructed and evaluated.•It was the first time for this method who had accurate identification ability to be used to screen active ingredient from traditional Chinese medicine.•This method was time saving, simple and needn’t complex pre-separation and extraction steps.