Cloned Calves from Chromatin Remodeled In Vitro

We have developed a novel system for remodeling mammalian somatic nuclei in vitro prior to cloning by nuclear transplantation. The system involves permeabilization of the donor cell and chromatin condensation in a mitotic cell extract to promote removal of nuclear factors solubilized during chromoso...

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Veröffentlicht in:	Biology of reproduction 2004-01, Vol.70 (1), p.146-153
Hauptverfasser:	Sullivan, E J, Kasinathan, S, Kasinathan, P, Robl, J M, Collas, P
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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