Optical biosensor optimized for continuous in-line glucose monitoring in animal cell culture
Biosensors for continuous glucose monitoring in bioreactors could provide a valuable tool for optimizing culture conditions in biotechnological applications. We have developed an optical biosensor for long-term continuous glucose monitoring and demonstrated a tight glucose level control during cell...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2017-09, Vol.409 (24), p.5711-5721 |
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| creator | Tric, Mircea Lederle, Mario Neuner, Lisa Dolgowjasow, Igor Wiedemann, Philipp Wölfl, Stefan Werner, Tobias |
| description | Biosensors for continuous glucose monitoring in bioreactors could provide a valuable tool for optimizing culture conditions in biotechnological applications. We have developed an optical biosensor for long-term continuous glucose monitoring and demonstrated a tight glucose level control during cell culture in disposable bioreactors. The in-line sensor is based on a commercially available oxygen sensor that is coated with cross-linked glucose oxidase (GOD). The dynamic range of the sensor was tuned by a hydrophilic perforated diffusion membrane with an optimized permeability for glucose and oxygen. The biosensor was thoroughly characterized by experimental data and numerical simulations, which enabled insights into the internal concentration profile of the deactivating by-product hydrogen peroxide. The simulations were carried out with a one-dimensional biosensor model and revealed that, in addition to the internal hydrogen peroxide concentration, the turnover rate of the enzyme GOD plays a crucial role for biosensor stability. In the light of this finding, the glucose sensor was optimized to reach a long functional stability (>52 days) under continuous glucose monitoring conditions with a dynamic range of 0–20 mM and a response time of
t
90
≤ 10 min. In addition, we demonstrated that the sensor was sterilizable with beta and UV irradiation and only subjected to minor cross sensitivity to oxygen, when an oxygen reference sensor was applied.
Graphical abstract
Measuring setup of a glucose biosensor in a shake flask for continuous glucose monitoring in mammalian cell culture |
| doi_str_mv | 10.1007/s00216-017-0511-7 |
| format | Article |
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t
90
≤ 10 min. In addition, we demonstrated that the sensor was sterilizable with beta and UV irradiation and only subjected to minor cross sensitivity to oxygen, when an oxygen reference sensor was applied.
Graphical abstract
Measuring setup of a glucose biosensor in a shake flask for continuous glucose monitoring in mammalian cell culture</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-017-0511-7</identifier><identifier>PMID: 28730310</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Beta rays ; Biochemistry ; Bioreactors ; Biosensors ; Biotechnology ; Blood sugar monitoring ; Cell culture ; Cells ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Computer simulation ; Crosslinking ; Darbepoetin alfa ; Deactivation ; Dextrose ; Dynamic range ; Dynamic stability ; Food Science ; Glucose ; Glucose monitoring ; Glucose oxidase ; Hydrogen peroxide ; Irradiation ; Laboratory Medicine ; Mathematical models ; Membrane permeability ; Monitoring ; Monitoring/Environmental Analysis ; Oxidases ; Oxygen ; Oxygen probes ; Permeability ; Research Paper ; Response time ; Sensors ; Turnover rate ; Ultraviolet radiation</subject><ispartof>Analytical and bioanalytical chemistry, 2017-09, Vol.409 (24), p.5711-5721</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-f7dddab2c711463ff8f16e9d018b75affdf045d55a790bf06e8de325ed90c6793</citedby><cites>FETCH-LOGICAL-c476t-f7dddab2c711463ff8f16e9d018b75affdf045d55a790bf06e8de325ed90c6793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-017-0511-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-017-0511-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28730310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tric, Mircea</creatorcontrib><creatorcontrib>Lederle, Mario</creatorcontrib><creatorcontrib>Neuner, Lisa</creatorcontrib><creatorcontrib>Dolgowjasow, Igor</creatorcontrib><creatorcontrib>Wiedemann, Philipp</creatorcontrib><creatorcontrib>Wölfl, Stefan</creatorcontrib><creatorcontrib>Werner, Tobias</creatorcontrib><title>Optical biosensor optimized for continuous in-line glucose monitoring in animal cell culture</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Biosensors for continuous glucose monitoring in bioreactors could provide a valuable tool for optimizing culture conditions in biotechnological applications. We have developed an optical biosensor for long-term continuous glucose monitoring and demonstrated a tight glucose level control during cell culture in disposable bioreactors. The in-line sensor is based on a commercially available oxygen sensor that is coated with cross-linked glucose oxidase (GOD). The dynamic range of the sensor was tuned by a hydrophilic perforated diffusion membrane with an optimized permeability for glucose and oxygen. The biosensor was thoroughly characterized by experimental data and numerical simulations, which enabled insights into the internal concentration profile of the deactivating by-product hydrogen peroxide. The simulations were carried out with a one-dimensional biosensor model and revealed that, in addition to the internal hydrogen peroxide concentration, the turnover rate of the enzyme GOD plays a crucial role for biosensor stability. In the light of this finding, the glucose sensor was optimized to reach a long functional stability (>52 days) under continuous glucose monitoring conditions with a dynamic range of 0–20 mM and a response time of
t
90
≤ 10 min. In addition, we demonstrated that the sensor was sterilizable with beta and UV irradiation and only subjected to minor cross sensitivity to oxygen, when an oxygen reference sensor was applied.
Graphical abstract
Measuring setup of a glucose biosensor in a shake flask for continuous glucose monitoring in mammalian cell culture</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Beta rays</subject><subject>Biochemistry</subject><subject>Bioreactors</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood sugar monitoring</subject><subject>Cell culture</subject><subject>Cells</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Computer simulation</subject><subject>Crosslinking</subject><subject>Darbepoetin alfa</subject><subject>Deactivation</subject><subject>Dextrose</subject><subject>Dynamic range</subject><subject>Dynamic stability</subject><subject>Food Science</subject><subject>Glucose</subject><subject>Glucose monitoring</subject><subject>Glucose oxidase</subject><subject>Hydrogen peroxide</subject><subject>Irradiation</subject><subject>Laboratory Medicine</subject><subject>Mathematical models</subject><subject>Membrane permeability</subject><subject>Monitoring</subject><subject>Monitoring/Environmental Analysis</subject><subject>Oxidases</subject><subject>Oxygen</subject><subject>Oxygen probes</subject><subject>Permeability</subject><subject>Research Paper</subject><subject>Response time</subject><subject>Sensors</subject><subject>Turnover rate</subject><subject>Ultraviolet 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Lisa</au><au>Dolgowjasow, Igor</au><au>Wiedemann, Philipp</au><au>Wölfl, Stefan</au><au>Werner, Tobias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical biosensor optimized for continuous in-line glucose monitoring in animal cell culture</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>409</volume><issue>24</issue><spage>5711</spage><epage>5721</epage><pages>5711-5721</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Biosensors for continuous glucose monitoring in bioreactors could provide a valuable tool for optimizing culture conditions in biotechnological applications. We have developed an optical biosensor for long-term continuous glucose monitoring and demonstrated a tight glucose level control during cell culture in disposable bioreactors. The in-line sensor is based on a commercially available oxygen sensor that is coated with cross-linked glucose oxidase (GOD). The dynamic range of the sensor was tuned by a hydrophilic perforated diffusion membrane with an optimized permeability for glucose and oxygen. The biosensor was thoroughly characterized by experimental data and numerical simulations, which enabled insights into the internal concentration profile of the deactivating by-product hydrogen peroxide. The simulations were carried out with a one-dimensional biosensor model and revealed that, in addition to the internal hydrogen peroxide concentration, the turnover rate of the enzyme GOD plays a crucial role for biosensor stability. In the light of this finding, the glucose sensor was optimized to reach a long functional stability (>52 days) under continuous glucose monitoring conditions with a dynamic range of 0–20 mM and a response time of
t
90
≤ 10 min. In addition, we demonstrated that the sensor was sterilizable with beta and UV irradiation and only subjected to minor cross sensitivity to oxygen, when an oxygen reference sensor was applied.
Graphical abstract
Measuring setup of a glucose biosensor in a shake flask for continuous glucose monitoring in mammalian cell culture</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28730310</pmid><doi>10.1007/s00216-017-0511-7</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Springer journals |
| subjects | Analysis Analytical Chemistry Beta rays Biochemistry Bioreactors Biosensors Biotechnology Blood sugar monitoring Cell culture Cells Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Computer simulation Crosslinking Darbepoetin alfa Deactivation Dextrose Dynamic range Dynamic stability Food Science Glucose Glucose monitoring Glucose oxidase Hydrogen peroxide Irradiation Laboratory Medicine Mathematical models Membrane permeability Monitoring Monitoring/Environmental Analysis Oxidases Oxygen Oxygen probes Permeability Research Paper Response time Sensors Turnover rate Ultraviolet radiation |
| title | Optical biosensor optimized for continuous in-line glucose monitoring in animal cell culture |
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