Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum
The sensitive olfactory system is necessary for survival of insects. Odorant receptors (ORs) are located on the dendrites of olfactory receptor neurons and play a critical role in odor detection. Insect ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two‐...
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| Veröffentlicht in: | Insect science 2019-02, Vol.26 (1), p.58-67 |
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| container_title | Insect science |
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| creator | Zhang, Rui‐Bin Liu, Yang Yan, Shan‐Chun Wang, Gui‐Rong |
| description | The sensitive olfactory system is necessary for survival of insects. Odorant receptors (ORs) are located on the dendrites of olfactory receptor neurons and play a critical role in odor detection. Insect ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two‐electrode voltage‐clamp (TEVC) electrophysiological recording. Here, we have identified a novel OR in the pea aphid, Acyrthosiphon pisum, then we cloned and named it ApisOR4. We analyzed the ApisOR4 tissue expression patterns and found expression only in antennae tissues. Further functional analysis using TEVC revealed that ApisOR4 is broadly tuned to eight volatiles, which elicit electrophysiological response in pea aphid antennae. This study provides an initial functional analysis of aphid ORs and identifies candidate volatiles to be used in developing new strategies for aphid control. |
| doi_str_mv | 10.1111/1744-7917.12510 |
| format | Article |
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Odorant receptors (ORs) are located on the dendrites of olfactory receptor neurons and play a critical role in odor detection. Insect ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two‐electrode voltage‐clamp (TEVC) electrophysiological recording. Here, we have identified a novel OR in the pea aphid, Acyrthosiphon pisum, then we cloned and named it ApisOR4. We analyzed the ApisOR4 tissue expression patterns and found expression only in antennae tissues. Further functional analysis using TEVC revealed that ApisOR4 is broadly tuned to eight volatiles, which elicit electrophysiological response in pea aphid antennae. This study provides an initial functional analysis of aphid ORs and identifies candidate volatiles to be used in developing new strategies for aphid control.</description><identifier>ISSN: 1672-9609</identifier><identifier>EISSN: 1744-7917</identifier><identifier>DOI: 10.1111/1744-7917.12510</identifier><identifier>PMID: 28730637</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>Acyrthosiphon pisum ; Amino Acid Sequence ; Animals ; Antennae ; Aphidoidea ; Aphids - genetics ; Aphids - metabolism ; Arthropod Antennae - metabolism ; Dendrites ; electroantennogram ; Electrophysiological recording ; Functional analysis ; Gene Expression ; heterologous expression ; Insects ; Nose ; odorant receptor ; Odorant receptors ; Odors ; Olfactory receptor neurons ; Olfactory system ; Patch-Clamp Techniques ; Receptors ; Receptors, Odorant - metabolism ; Recording ; Sequence Analysis, DNA ; Smell ; Tissues ; Volatiles ; Xenopus</subject><ispartof>Insect science, 2019-02, Vol.26 (1), p.58-67</ispartof><rights>2017 Institute of Zoology, Chinese Academy of Sciences</rights><rights>2017 Institute of Zoology, Chinese Academy of Sciences.</rights><rights>2019 Institute of Zoology, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4120-621d82c3b9d5bb6bacfd359e173c3d1da456ea08707ad5cbca3cb818cb952e7e3</citedby><cites>FETCH-LOGICAL-c4120-621d82c3b9d5bb6bacfd359e173c3d1da456ea08707ad5cbca3cb818cb952e7e3</cites><orcidid>0000-0002-3794-0106</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1744-7917.12510$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1744-7917.12510$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28730637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Rui‐Bin</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Yan, Shan‐Chun</creatorcontrib><creatorcontrib>Wang, Gui‐Rong</creatorcontrib><title>Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum</title><title>Insect science</title><addtitle>Insect Sci</addtitle><description>The sensitive olfactory system is necessary for survival of insects. Odorant receptors (ORs) are located on the dendrites of olfactory receptor neurons and play a critical role in odor detection. Insect ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two‐electrode voltage‐clamp (TEVC) electrophysiological recording. Here, we have identified a novel OR in the pea aphid, Acyrthosiphon pisum, then we cloned and named it ApisOR4. We analyzed the ApisOR4 tissue expression patterns and found expression only in antennae tissues. Further functional analysis using TEVC revealed that ApisOR4 is broadly tuned to eight volatiles, which elicit electrophysiological response in pea aphid antennae. This study provides an initial functional analysis of aphid ORs and identifies candidate volatiles to be used in developing new strategies for aphid control.</description><subject>Acyrthosiphon pisum</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antennae</subject><subject>Aphidoidea</subject><subject>Aphids - genetics</subject><subject>Aphids - metabolism</subject><subject>Arthropod Antennae - metabolism</subject><subject>Dendrites</subject><subject>electroantennogram</subject><subject>Electrophysiological recording</subject><subject>Functional analysis</subject><subject>Gene Expression</subject><subject>heterologous expression</subject><subject>Insects</subject><subject>Nose</subject><subject>odorant receptor</subject><subject>Odorant receptors</subject><subject>Odors</subject><subject>Olfactory receptor neurons</subject><subject>Olfactory system</subject><subject>Patch-Clamp Techniques</subject><subject>Receptors</subject><subject>Receptors, Odorant - metabolism</subject><subject>Recording</subject><subject>Sequence Analysis, DNA</subject><subject>Smell</subject><subject>Tissues</subject><subject>Volatiles</subject><subject>Xenopus</subject><issn>1672-9609</issn><issn>1744-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTlPxDAQhS0E4q7pkCUaCgI-Eh8lWnGshKAAasuxHdYoGwc7EVp-PV4CFDRM4xn7e0_yGwCOMDrHuS4wL8uCS8zPMakw2gC7vzebuWecFJIhuQP2UnpFiEoiyTbYIYJTxCjfBS9z67rBN97owYcO6s7CZuzMetAtNAsdtRlc9B_Te2gyAoMNUXcDjM64fggR-g72TkPdL7w9g5dmFYdFSL5fZEnv07g8AFuNbpM7_D73wfP11dPstrh7uJnPLu8KU2KCCkawFcTQWtqqrlmtTWNpJR3m1FCLrS4r5jQSHHFtK1MbTU0tsDC1rIjjju6D08m3j-FtdGlQS5-Ma1vduTAmhSUhFRJC8oye_EFfwxjzr5MimBEmBC1Zpi4mysSQUnSN6qNf6rhSGKn1DtQ6cbVOXH3tICuOv33HeunsL_8TegaqCXj3rVv956fm94-T8SfFOZIM</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Zhang, Rui‐Bin</creator><creator>Liu, Yang</creator><creator>Yan, Shan‐Chun</creator><creator>Wang, Gui‐Rong</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3794-0106</orcidid></search><sort><creationdate>201902</creationdate><title>Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum</title><author>Zhang, Rui‐Bin ; Liu, Yang ; Yan, Shan‐Chun ; Wang, Gui‐Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4120-621d82c3b9d5bb6bacfd359e173c3d1da456ea08707ad5cbca3cb818cb952e7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acyrthosiphon pisum</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antennae</topic><topic>Aphidoidea</topic><topic>Aphids - genetics</topic><topic>Aphids - metabolism</topic><topic>Arthropod Antennae - metabolism</topic><topic>Dendrites</topic><topic>electroantennogram</topic><topic>Electrophysiological recording</topic><topic>Functional analysis</topic><topic>Gene Expression</topic><topic>heterologous expression</topic><topic>Insects</topic><topic>Nose</topic><topic>odorant receptor</topic><topic>Odorant receptors</topic><topic>Odors</topic><topic>Olfactory receptor neurons</topic><topic>Olfactory system</topic><topic>Patch-Clamp Techniques</topic><topic>Receptors</topic><topic>Receptors, Odorant - metabolism</topic><topic>Recording</topic><topic>Sequence Analysis, DNA</topic><topic>Smell</topic><topic>Tissues</topic><topic>Volatiles</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Rui‐Bin</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Yan, Shan‐Chun</creatorcontrib><creatorcontrib>Wang, Gui‐Rong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Rui‐Bin</au><au>Liu, Yang</au><au>Yan, Shan‐Chun</au><au>Wang, Gui‐Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum</atitle><jtitle>Insect science</jtitle><addtitle>Insect Sci</addtitle><date>2019-02</date><risdate>2019</risdate><volume>26</volume><issue>1</issue><spage>58</spage><epage>67</epage><pages>58-67</pages><issn>1672-9609</issn><eissn>1744-7917</eissn><abstract>The sensitive olfactory system is necessary for survival of insects. Odorant receptors (ORs) are located on the dendrites of olfactory receptor neurons and play a critical role in odor detection. Insect ORs are functionally analyzed via heterologous expression in a Xenopus oocyte system using a two‐electrode voltage‐clamp (TEVC) electrophysiological recording. Here, we have identified a novel OR in the pea aphid, Acyrthosiphon pisum, then we cloned and named it ApisOR4. We analyzed the ApisOR4 tissue expression patterns and found expression only in antennae tissues. Further functional analysis using TEVC revealed that ApisOR4 is broadly tuned to eight volatiles, which elicit electrophysiological response in pea aphid antennae. 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| subjects | Acyrthosiphon pisum Amino Acid Sequence Animals Antennae Aphidoidea Aphids - genetics Aphids - metabolism Arthropod Antennae - metabolism Dendrites electroantennogram Electrophysiological recording Functional analysis Gene Expression heterologous expression Insects Nose odorant receptor Odorant receptors Odors Olfactory receptor neurons Olfactory system Patch-Clamp Techniques Receptors Receptors, Odorant - metabolism Recording Sequence Analysis, DNA Smell Tissues Volatiles Xenopus |
| title | Identification and functional characterization of an odorant receptor in pea aphid, Acyrthosiphon pisum |
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