Fabrication of protein G LB film for immunoglobulin G immobilization
For the development of an immunosensor and protein chip with high efficiency, the thin film of protein G to has specific affinity to the Fc portion of immunoglobulin G (IgG) was fabricated by using the Langmuir–Blodgett (LB) technique. The formation of protein G LB film on a solid surface was confir...
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| Veröffentlicht in: | Materials Science & Engineering C 2004-01, Vol.24 (1), p.65-69 |
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| container_end_page | 69 |
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| container_issue | 1 |
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| container_title | Materials Science & Engineering C |
| container_volume | 24 |
| creator | Oh, Byung-Keun Chun, Bum Suk Park, Kwang-Won Lee, Woochang Lee, Won Hong Choi, Jeong-Woo |
| description | For the development of an immunosensor and protein chip with high efficiency, the thin film of protein G to has specific affinity to the Fc portion of immunoglobulin G (IgG) was fabricated by using the Langmuir–Blodgett (LB) technique. The formation of protein G LB film on a solid surface was confirmed by surface plasmon resonance (SPR), and the surface morphologies of the protein G LB film were observed by using atomic force microscopy (AFM). The IgG binding to protein G LB film and antigen binding to immobilized IgG were verified by SPR measurement. |
| doi_str_mv | 10.1016/j.msec.2003.09.065 |
| format | Article |
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| ispartof | Materials Science & Engineering C, 2004-01, Vol.24 (1), p.65-69 |
| issn | 0928-4931 1873-0191 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Atomic force microscopy Langmuir–Blodgett technique Protein G Surface plasmon resonance |
| title | Fabrication of protein G LB film for immunoglobulin G immobilization |
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