16S-ARDRA, a Tool for Identification of Lactic Acid Bacteria Isolated from Grape Must and Wine
Lactic acid bacteria (LAB) are found in a great variety of habitats, including grape must and wines. There is a close relationship between the species of LAB which develop during fermentation and the eventual quality of the wine. For these reasons analytical techniques allowing fast and reliable ide...
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| Veröffentlicht in: | Systematic and applied microbiology 2003-09, Vol.26 (3), p.412-422 |
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| creator | Rodas, Ana María Ferrer, Sergi Pardo, Isabel |
| description | Lactic acid bacteria (LAB) are found in a great variety of habitats, including grape must and wines. There is a close relationship between the species of LAB which develop during fermentation and the eventual quality of the wine. For these reasons analytical techniques allowing fast and reliable identification of wine LAB are needed.
In this work a simple and accurate protocol for identifying species of LAB isolated from grape must and wine is presented. This protocol is based on the amplification, directly from colony, of 16S rDNA and later digestion with one of the following restriction enzymes
Bfa I,
Mse I and
Alu I. A sequential use of the three enzymes is proposed to simplify LAB wine identification, first
Mse I, then
Bfa I and finally, if necessary,
Alu I digestion. The technique was able to discriminate 32 of the 36 LAB reference species tested and allowed the identification of 342 isolates from musts and wines. The isolates belonged to the species:
Lactobacillus brevis,
L. collinoides,
L. coryniformis,
L. hilgardii,
L. mali,
L. paracasei,
Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus parvulus and
P. pentosaceus. |
| doi_str_mv | 10.1078/072320203322497446 |
| format | Article |
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In this work a simple and accurate protocol for identifying species of LAB isolated from grape must and wine is presented. This protocol is based on the amplification, directly from colony, of 16S rDNA and later digestion with one of the following restriction enzymes
Bfa I,
Mse I and
Alu I. A sequential use of the three enzymes is proposed to simplify LAB wine identification, first
Mse I, then
Bfa I and finally, if necessary,
Alu I digestion. The technique was able to discriminate 32 of the 36 LAB reference species tested and allowed the identification of 342 isolates from musts and wines. The isolates belonged to the species:
Lactobacillus brevis,
L. collinoides,
L. coryniformis,
L. hilgardii,
L. mali,
L. paracasei,
Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus parvulus and
P. pentosaceus.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1078/072320203322497446</identifier><identifier>PMID: 14529184</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>16S rDNA ; ARDRA ; Biological and medical sciences ; DNA Fingerprinting ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; DNA, Ribosomal - analysis ; DNA, Ribosomal - genetics ; Fermentation ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Gram-Positive Bacteria - classification ; Gram-Positive Bacteria - isolation & purification ; Gram-Positive Bacteria - metabolism ; Hexoses - metabolism ; Lactic Acid - biosynthesis ; lactic acid bacteria ; Lactobacillus - classification ; Lactobacillus - isolation & purification ; Lactobacillus - metabolism ; Lactobacillus brevis ; Lactobacillus collinoides ; Lactobacillus coryniformis ; Lactobacillus hilgardii ; Lactobacillus mali ; Lactobacillus paracasei ; Leuconostoc - classification ; Leuconostoc - isolation & purification ; Leuconostoc - metabolism ; Leuconostoc mesenteroides ; Microbiology ; molecular identification ; Oenococcus oeni ; Pediococcus - classification ; Pediococcus - isolation & purification ; Pediococcus - metabolism ; Pediococcus parvulus ; Pediococcus pentosaceus ; Phylogeny ; Polymerase Chain Reaction ; wine ; Wine - microbiology</subject><ispartof>Systematic and applied microbiology, 2003-09, Vol.26 (3), p.412-422</ispartof><rights>2003 Urban & Fischer Verlag</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Urban & Fischer Verlag Sep 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-fca38f4fcf67696fec6f0ab4042a98ca50b79343fc8d9ed6e5b1d21a930efb6c3</citedby><cites>FETCH-LOGICAL-c506t-fca38f4fcf67696fec6f0ab4042a98ca50b79343fc8d9ed6e5b1d21a930efb6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/208457370?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994,64384,64386,64388,72240</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15144358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14529184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodas, Ana María</creatorcontrib><creatorcontrib>Ferrer, Sergi</creatorcontrib><creatorcontrib>Pardo, Isabel</creatorcontrib><title>16S-ARDRA, a Tool for Identification of Lactic Acid Bacteria Isolated from Grape Must and Wine</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>Lactic acid bacteria (LAB) are found in a great variety of habitats, including grape must and wines. There is a close relationship between the species of LAB which develop during fermentation and the eventual quality of the wine. For these reasons analytical techniques allowing fast and reliable identification of wine LAB are needed.
In this work a simple and accurate protocol for identifying species of LAB isolated from grape must and wine is presented. This protocol is based on the amplification, directly from colony, of 16S rDNA and later digestion with one of the following restriction enzymes
Bfa I,
Mse I and
Alu I. A sequential use of the three enzymes is proposed to simplify LAB wine identification, first
Mse I, then
Bfa I and finally, if necessary,
Alu I digestion. The technique was able to discriminate 32 of the 36 LAB reference species tested and allowed the identification of 342 isolates from musts and wines. The isolates belonged to the species:
Lactobacillus brevis,
L. collinoides,
L. coryniformis,
L. hilgardii,
L. mali,
L. paracasei,
Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus parvulus and
P. pentosaceus.</description><subject>16S rDNA</subject><subject>ARDRA</subject><subject>Biological and medical sciences</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - analysis</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fermentation</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gram-Positive Bacteria - classification</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>Gram-Positive Bacteria - metabolism</subject><subject>Hexoses - metabolism</subject><subject>Lactic Acid - biosynthesis</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - isolation & purification</subject><subject>Lactobacillus - metabolism</subject><subject>Lactobacillus brevis</subject><subject>Lactobacillus collinoides</subject><subject>Lactobacillus coryniformis</subject><subject>Lactobacillus hilgardii</subject><subject>Lactobacillus mali</subject><subject>Lactobacillus paracasei</subject><subject>Leuconostoc - classification</subject><subject>Leuconostoc - isolation & purification</subject><subject>Leuconostoc - metabolism</subject><subject>Leuconostoc mesenteroides</subject><subject>Microbiology</subject><subject>molecular identification</subject><subject>Oenococcus oeni</subject><subject>Pediococcus - classification</subject><subject>Pediococcus - isolation & purification</subject><subject>Pediococcus - metabolism</subject><subject>Pediococcus parvulus</subject><subject>Pediococcus pentosaceus</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>wine</subject><subject>Wine - microbiology</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90E2LFDEQBuAgiju7-gc8SBD0ZGu-Op2Al3FX14ERYV3xZqhOKpClpzMm3YL_3h5mYEHBU9XhqeLlJeQZZ28468xb1gkpmGBSCqFsp5R-QFZcc9Mwa9RDsjqA5iDOyHmtd4xxZTV_TM64aoXlRq3ID66_Nuubq5v1awr0NueBxlzoJuA4pZg8TCmPNEe6BT8lT9c-Bfp-2bEkoJuaB5gw0Fjyjl4X2CP9PNeJwhjo9zTiE_IowlDx6WlekG8fP9xefmq2X643l-tt41umpyZ6kCaq6KPutNURvY4MesWUAGs8tKzvrFQyehMsBo1tz4PgYCXD2GsvL8ir4999yT9nrJPbpepxGGDEPFfHreBaS7PAF3_BuzyXccnmBDOq7WTHFiSOyJdca8Ho9iXtoPx2nLlD9e7f6pej56fPc7_DcH9y6noBL08AqochFhh9qveu5UrJ9hDx3dHhUtivhMVVn3D0GFJBP7mQ0_9y_AFbZJuH</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Rodas, Ana María</creator><creator>Ferrer, Sergi</creator><creator>Pardo, Isabel</creator><general>Elsevier GmbH</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20030901</creationdate><title>16S-ARDRA, a Tool for Identification of Lactic Acid Bacteria Isolated from Grape Must and Wine</title><author>Rodas, Ana María ; Ferrer, Sergi ; Pardo, Isabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-fca38f4fcf67696fec6f0ab4042a98ca50b79343fc8d9ed6e5b1d21a930efb6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>16S rDNA</topic><topic>ARDRA</topic><topic>Biological and medical sciences</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - analysis</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fermentation</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gram-Positive Bacteria - classification</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>Gram-Positive Bacteria - metabolism</topic><topic>Hexoses - metabolism</topic><topic>Lactic Acid - biosynthesis</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus - classification</topic><topic>Lactobacillus - isolation & purification</topic><topic>Lactobacillus - metabolism</topic><topic>Lactobacillus brevis</topic><topic>Lactobacillus collinoides</topic><topic>Lactobacillus coryniformis</topic><topic>Lactobacillus hilgardii</topic><topic>Lactobacillus mali</topic><topic>Lactobacillus paracasei</topic><topic>Leuconostoc - classification</topic><topic>Leuconostoc - isolation & purification</topic><topic>Leuconostoc - metabolism</topic><topic>Leuconostoc mesenteroides</topic><topic>Microbiology</topic><topic>molecular identification</topic><topic>Oenococcus oeni</topic><topic>Pediococcus - classification</topic><topic>Pediococcus - isolation & purification</topic><topic>Pediococcus - metabolism</topic><topic>Pediococcus parvulus</topic><topic>Pediococcus pentosaceus</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>wine</topic><topic>Wine - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodas, Ana María</creatorcontrib><creatorcontrib>Ferrer, Sergi</creatorcontrib><creatorcontrib>Pardo, Isabel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodas, Ana María</au><au>Ferrer, Sergi</au><au>Pardo, Isabel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>16S-ARDRA, a Tool for Identification of Lactic Acid Bacteria Isolated from Grape Must and Wine</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>26</volume><issue>3</issue><spage>412</spage><epage>422</epage><pages>412-422</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>Lactic acid bacteria (LAB) are found in a great variety of habitats, including grape must and wines. There is a close relationship between the species of LAB which develop during fermentation and the eventual quality of the wine. For these reasons analytical techniques allowing fast and reliable identification of wine LAB are needed.
In this work a simple and accurate protocol for identifying species of LAB isolated from grape must and wine is presented. This protocol is based on the amplification, directly from colony, of 16S rDNA and later digestion with one of the following restriction enzymes
Bfa I,
Mse I and
Alu I. A sequential use of the three enzymes is proposed to simplify LAB wine identification, first
Mse I, then
Bfa I and finally, if necessary,
Alu I digestion. The technique was able to discriminate 32 of the 36 LAB reference species tested and allowed the identification of 342 isolates from musts and wines. The isolates belonged to the species:
Lactobacillus brevis,
L. collinoides,
L. coryniformis,
L. hilgardii,
L. mali,
L. paracasei,
Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus parvulus and
P. pentosaceus.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>14529184</pmid><doi>10.1078/072320203322497446</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0723-2020 |
| ispartof | Systematic and applied microbiology, 2003-09, Vol.26 (3), p.412-422 |
| issn | 0723-2020 1618-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19216638 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | 16S rDNA ARDRA Biological and medical sciences DNA Fingerprinting DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Ribosomal - analysis DNA, Ribosomal - genetics Fermentation Food Microbiology Fundamental and applied biological sciences. Psychology Gram-Positive Bacteria - classification Gram-Positive Bacteria - isolation & purification Gram-Positive Bacteria - metabolism Hexoses - metabolism Lactic Acid - biosynthesis lactic acid bacteria Lactobacillus - classification Lactobacillus - isolation & purification Lactobacillus - metabolism Lactobacillus brevis Lactobacillus collinoides Lactobacillus coryniformis Lactobacillus hilgardii Lactobacillus mali Lactobacillus paracasei Leuconostoc - classification Leuconostoc - isolation & purification Leuconostoc - metabolism Leuconostoc mesenteroides Microbiology molecular identification Oenococcus oeni Pediococcus - classification Pediococcus - isolation & purification Pediococcus - metabolism Pediococcus parvulus Pediococcus pentosaceus Phylogeny Polymerase Chain Reaction wine Wine - microbiology |
| title | 16S-ARDRA, a Tool for Identification of Lactic Acid Bacteria Isolated from Grape Must and Wine |
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