Xanthomonas type III effector XopD targets SUMO‐conjugated proteins in planta
Summary Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provi...
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| Veröffentlicht in: | Molecular microbiology 2003-10, Vol.50 (2), p.377-389 |
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| container_title | Molecular microbiology |
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| creator | Hotson, Andrew Chosed, Renee Shu, Hongjun Orth, Kim Mudgett, Mary Beth |
| description | Summary
Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provide direct evidence for a functional role for a phytopathogenic bacterial TTSS effector in planta by demonstrating that the Xcv effector XopD encodes an active cysteine protease with plant‐specific SUMO substrate specificity. XopD is injected into plant cells by the TTSS during Xcv pathogenesis, translocated to subnuclear foci and hydrolyses SUMO‐conjugated proteins in vivo. Our studies suggest that XopD mimics endogenous plant SUMO isopeptidases to interfere with the regulation of host proteins during Xcv infection. |
| doi_str_mv | 10.1046/j.1365-2958.2003.03730.x |
| format | Article |
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Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provide direct evidence for a functional role for a phytopathogenic bacterial TTSS effector in planta by demonstrating that the Xcv effector XopD encodes an active cysteine protease with plant‐specific SUMO substrate specificity. XopD is injected into plant cells by the TTSS during Xcv pathogenesis, translocated to subnuclear foci and hydrolyses SUMO‐conjugated proteins in vivo. Our studies suggest that XopD mimics endogenous plant SUMO isopeptidases to interfere with the regulation of host proteins during Xcv infection.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2003.03730.x</identifier><identifier>PMID: 14617166</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Bacterial Proteins - metabolism ; Biological Transport, Active ; Cysteine Endopeptidases - metabolism ; Plants - microbiology ; Protein Transport - physiology ; Recombinant Proteins - pharmacology ; secretion system (Type III) ; SUMO-1 Protein - metabolism ; Xanthomonas campestris ; Xanthomonas campestris - enzymology ; Xanthomonas campestris - metabolism ; XopD protein</subject><ispartof>Molecular microbiology, 2003-10, Vol.50 (2), p.377-389</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Oct 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5080-ef0d5bfd1361f564b706d821be71599c027bd0a9986c38d9b7d1b6918cd372fa3</citedby><cites>FETCH-LOGICAL-c5080-ef0d5bfd1361f564b706d821be71599c027bd0a9986c38d9b7d1b6918cd372fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2003.03730.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2003.03730.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14617166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hotson, Andrew</creatorcontrib><creatorcontrib>Chosed, Renee</creatorcontrib><creatorcontrib>Shu, Hongjun</creatorcontrib><creatorcontrib>Orth, Kim</creatorcontrib><creatorcontrib>Mudgett, Mary Beth</creatorcontrib><title>Xanthomonas type III effector XopD targets SUMO‐conjugated proteins in planta</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provide direct evidence for a functional role for a phytopathogenic bacterial TTSS effector in planta by demonstrating that the Xcv effector XopD encodes an active cysteine protease with plant‐specific SUMO substrate specificity. XopD is injected into plant cells by the TTSS during Xcv pathogenesis, translocated to subnuclear foci and hydrolyses SUMO‐conjugated proteins in vivo. Our studies suggest that XopD mimics endogenous plant SUMO isopeptidases to interfere with the regulation of host proteins during Xcv infection.</description><subject>Bacterial Proteins - metabolism</subject><subject>Biological Transport, Active</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>Plants - microbiology</subject><subject>Protein Transport - physiology</subject><subject>Recombinant Proteins - pharmacology</subject><subject>secretion system (Type III)</subject><subject>SUMO-1 Protein - metabolism</subject><subject>Xanthomonas campestris</subject><subject>Xanthomonas campestris - enzymology</subject><subject>Xanthomonas campestris - metabolism</subject><subject>XopD protein</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtO4zAUhi3ECMrlFZDFgl0yx3bj2AsWiNtEoupiQOrOcmIHErVxiBPR7ngEnpEnwZlWMxKrWZ0jne_8-vUhhAnEBKb8Zx0TxpOIykTEFIDFwFIG8XoPTf4e9tEEZAIRE3RxiI68rwEIA84O0CGZcpISzidovtBN_-JWrtEe95vW4izLsC1LW_SuwwvX3uBed8-29_j302z--f5RuKYennVvDW4719uq8bhqcLsMSfoE_Sj10tvT3TxGT3e3j9e_oof5fXZ99RAVCQiIbAkmyUsT2pIy4dM8BW4EJblNSSJlATTNDWgpBS-YMDJPDcm5JKIwLKWlZsfoYpsbKrwO1vdqVfnCLkMJ6waviKQEhBQBPP8G1m7omtAtMDyhwKY0QGILFZ3zvrOlartqpbuNIqBG46pWo1g1ilWjcfXHuFqH17Nd_pCvrPn3uFMcgMst8FYt7ea_g9Vslo0b-wI41I8u</recordid><startdate>200310</startdate><enddate>200310</enddate><creator>Hotson, Andrew</creator><creator>Chosed, Renee</creator><creator>Shu, Hongjun</creator><creator>Orth, Kim</creator><creator>Mudgett, Mary Beth</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200310</creationdate><title>Xanthomonas type III effector XopD targets SUMO‐conjugated proteins in planta</title><author>Hotson, Andrew ; Chosed, Renee ; Shu, Hongjun ; Orth, Kim ; Mudgett, Mary Beth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5080-ef0d5bfd1361f564b706d821be71599c027bd0a9986c38d9b7d1b6918cd372fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacterial Proteins - metabolism</topic><topic>Biological Transport, Active</topic><topic>Cysteine Endopeptidases - metabolism</topic><topic>Plants - microbiology</topic><topic>Protein Transport - physiology</topic><topic>Recombinant Proteins - pharmacology</topic><topic>secretion system (Type III)</topic><topic>SUMO-1 Protein - metabolism</topic><topic>Xanthomonas campestris</topic><topic>Xanthomonas campestris - enzymology</topic><topic>Xanthomonas campestris - metabolism</topic><topic>XopD protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hotson, Andrew</creatorcontrib><creatorcontrib>Chosed, Renee</creatorcontrib><creatorcontrib>Shu, Hongjun</creatorcontrib><creatorcontrib>Orth, Kim</creatorcontrib><creatorcontrib>Mudgett, Mary Beth</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hotson, Andrew</au><au>Chosed, Renee</au><au>Shu, Hongjun</au><au>Orth, Kim</au><au>Mudgett, Mary Beth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Xanthomonas type III effector XopD targets SUMO‐conjugated proteins in planta</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2003-10</date><risdate>2003</risdate><volume>50</volume><issue>2</issue><spage>377</spage><epage>389</epage><pages>377-389</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Xanthomonas campestris pathovar vesicatoria (Xcv) uses the type III secretion system (TTSS) to inject effector proteins into cells of Solanaceous plants during pathogenesis. A number of Xcv TTSS effectors have been identified; however, their function in planta remains elusive. Here, we provide direct evidence for a functional role for a phytopathogenic bacterial TTSS effector in planta by demonstrating that the Xcv effector XopD encodes an active cysteine protease with plant‐specific SUMO substrate specificity. XopD is injected into plant cells by the TTSS during Xcv pathogenesis, translocated to subnuclear foci and hydrolyses SUMO‐conjugated proteins in vivo. Our studies suggest that XopD mimics endogenous plant SUMO isopeptidases to interfere with the regulation of host proteins during Xcv infection.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>14617166</pmid><doi>10.1046/j.1365-2958.2003.03730.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Bacterial Proteins - metabolism Biological Transport, Active Cysteine Endopeptidases - metabolism Plants - microbiology Protein Transport - physiology Recombinant Proteins - pharmacology secretion system (Type III) SUMO-1 Protein - metabolism Xanthomonas campestris Xanthomonas campestris - enzymology Xanthomonas campestris - metabolism XopD protein |
| title | Xanthomonas type III effector XopD targets SUMO‐conjugated proteins in planta |
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