Bacterial Adherence to Graft Tissues in Static and Flow Conditions
Abstract Objective Various conduits or stent-mounted valves are used as pulmonary valve graft tissues for Right Ventricular Outflow Tract reconstruction with good hemodynamic results. Valve replacement inherits an increased risk of infective endocarditis (IE). Recent observations rose awareness to I...
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| Veröffentlicht in: | The Journal of thoracic and cardiovascular surgery 2018-01, Vol.155 (1), p.325-332.e4 |
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| creator | Veloso, Tiago Rafael, PhD Claes, Jorien, PhD Van kerckhoven, Soetkin Ditkowski, Bartosz, PhD Hurtado-Aguilar, Luis G., MSc Jockenhoevel, Stefan, MD, PhD Mela, Petra, PhD Jashari, Ramadan, MD, PhD Gewillig, Marc, MD, PhD Hoylaerts, Marc F., PhD Meyns, Bart, MD, PhD Heying, Ruth, MD, PhD |
| description | Abstract Objective Various conduits or stent-mounted valves are used as pulmonary valve graft tissues for Right Ventricular Outflow Tract reconstruction with good hemodynamic results. Valve replacement inherits an increased risk of infective endocarditis (IE). Recent observations rose awareness to IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. The present study focuses on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE. Methods Staphylococcus aureus , S. epidermidis and Streptococcus sanguinis adhesion to bovine pericardium (BP) patch, bovine jugular vein (BJV) and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components. Results In general, similar bacteria numbers were recovered from CH and BJV tissue surface for all strains, especially in flow conditions. Static bacterial adhesion to CH wall was lower for S. sanguinis adhesion ( P |
| doi_str_mv | 10.1016/j.jtcvs.2017.06.014 |
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Valve replacement inherits an increased risk of infective endocarditis (IE). Recent observations rose awareness to IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. The present study focuses on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE. Methods Staphylococcus aureus , S. epidermidis and Streptococcus sanguinis adhesion to bovine pericardium (BP) patch, bovine jugular vein (BJV) and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components. Results In general, similar bacteria numbers were recovered from CH and BJV tissue surface for all strains, especially in flow conditions. Static bacterial adhesion to CH wall was lower for S. sanguinis adhesion ( P <0.05 vs. BP patch). Adhesion to BJV wall, CH wall and leaflet were found to be decreased for S. epidermidis in static conditions ( P <0.05 vs. BP patch). Bacterial adhesion under shear stress indicates a similar bacterial adhesion to all tissues, except for a decrease to BJV wall after S. sanguinis incubation. Microscopic analysis shows the importance of matrix component exposure for bacterial adherence to CH. Conclusions Our data exhibit evidence that the surface composition of BJV and CH tissues itself, bacterial surface proteins or shear forces per se are not the prime determinants during initial bacterial adherence.</description><identifier>ISSN: 0022-5223</identifier><identifier>EISSN: 1097-685X</identifier><identifier>DOI: 10.1016/j.jtcvs.2017.06.014</identifier><identifier>PMID: 28712577</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cardiothoracic Surgery ; congenital heart disease ; congenital heart disease surgery ; endocarditis ; prosthetic heart valve ; valved conduit</subject><ispartof>The Journal of thoracic and cardiovascular surgery, 2018-01, Vol.155 (1), p.325-332.e4</ispartof><rights>2017 The American Association for Thoracic Surgery</rights><rights>Copyright © 2017 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-4c642a32bb3c63a037cbc981c02f0a07089a78571e958bc41a42760fa0c153d93</citedby><cites>FETCH-LOGICAL-c459t-4c642a32bb3c63a037cbc981c02f0a07089a78571e958bc41a42760fa0c153d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jtcvs.2017.06.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28712577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Veloso, Tiago Rafael, PhD</creatorcontrib><creatorcontrib>Claes, Jorien, PhD</creatorcontrib><creatorcontrib>Van kerckhoven, Soetkin</creatorcontrib><creatorcontrib>Ditkowski, Bartosz, PhD</creatorcontrib><creatorcontrib>Hurtado-Aguilar, Luis G., MSc</creatorcontrib><creatorcontrib>Jockenhoevel, Stefan, MD, PhD</creatorcontrib><creatorcontrib>Mela, Petra, PhD</creatorcontrib><creatorcontrib>Jashari, Ramadan, MD, PhD</creatorcontrib><creatorcontrib>Gewillig, Marc, MD, PhD</creatorcontrib><creatorcontrib>Hoylaerts, Marc F., PhD</creatorcontrib><creatorcontrib>Meyns, Bart, MD, PhD</creatorcontrib><creatorcontrib>Heying, Ruth, MD, PhD</creatorcontrib><title>Bacterial Adherence to Graft Tissues in Static and Flow Conditions</title><title>The Journal of thoracic and cardiovascular surgery</title><addtitle>J Thorac Cardiovasc Surg</addtitle><description>Abstract Objective Various conduits or stent-mounted valves are used as pulmonary valve graft tissues for Right Ventricular Outflow Tract reconstruction with good hemodynamic results. Valve replacement inherits an increased risk of infective endocarditis (IE). Recent observations rose awareness to IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. The present study focuses on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE. Methods Staphylococcus aureus , S. epidermidis and Streptococcus sanguinis adhesion to bovine pericardium (BP) patch, bovine jugular vein (BJV) and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components. Results In general, similar bacteria numbers were recovered from CH and BJV tissue surface for all strains, especially in flow conditions. Static bacterial adhesion to CH wall was lower for S. sanguinis adhesion ( P <0.05 vs. BP patch). Adhesion to BJV wall, CH wall and leaflet were found to be decreased for S. epidermidis in static conditions ( P <0.05 vs. BP patch). Bacterial adhesion under shear stress indicates a similar bacterial adhesion to all tissues, except for a decrease to BJV wall after S. sanguinis incubation. Microscopic analysis shows the importance of matrix component exposure for bacterial adherence to CH. Conclusions Our data exhibit evidence that the surface composition of BJV and CH tissues itself, bacterial surface proteins or shear forces per se are not the prime determinants during initial bacterial adherence.</description><subject>Cardiothoracic Surgery</subject><subject>congenital heart disease</subject><subject>congenital heart disease surgery</subject><subject>endocarditis</subject><subject>prosthetic heart valve</subject><subject>valved conduit</subject><issn>0022-5223</issn><issn>1097-685X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS1ERZfCL0BCPnJJmLGT2D6A1K5oQarUQ1uJm-U4jnDIxsX2tuq_x2ELBy49zeW9eTPfI-QdQo2A3cepnrK9TzUDFDV0NWDzgmwQlKg62X5_STYAjFUtY_yYvE5pAgABqF6RYyYFslaIDTk7Mza76M1MT4cfLrrFOpoDvYhmzPTGp7R3ifqFXmeTvaVmGej5HB7oNiyDzz4s6Q05Gs2c3NuneUJuz7_cbL9Wl1cX37anl5VtWpWrxnYNM5z1PbcdN8CF7a2SaIGNYMplUhkhW4FOtbK3DZqGiQ5GAxZbPih-Qj4c9t7F8KtclfXOJ-vm2Swu7JNGVUgoxSQUKT9IbQwpRTfqu-h3Jj5qBL3C05P-A0-v8DR0usArrvdPAft-54Z_nr-0iuDTQeDKm_feRZ2sX4kNPjqb9RD8MwGf__Pb2S_emvmne3RpCvu4FIIadWIa9PXa31ofCo4oO8l_AxbglGU</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Veloso, Tiago Rafael, PhD</creator><creator>Claes, Jorien, PhD</creator><creator>Van kerckhoven, Soetkin</creator><creator>Ditkowski, Bartosz, PhD</creator><creator>Hurtado-Aguilar, Luis G., MSc</creator><creator>Jockenhoevel, Stefan, MD, PhD</creator><creator>Mela, Petra, PhD</creator><creator>Jashari, Ramadan, MD, PhD</creator><creator>Gewillig, Marc, MD, PhD</creator><creator>Hoylaerts, Marc F., PhD</creator><creator>Meyns, Bart, MD, PhD</creator><creator>Heying, Ruth, MD, PhD</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180101</creationdate><title>Bacterial Adherence to Graft Tissues in Static and Flow Conditions</title><author>Veloso, Tiago Rafael, PhD ; Claes, Jorien, PhD ; Van kerckhoven, Soetkin ; Ditkowski, Bartosz, PhD ; Hurtado-Aguilar, Luis G., MSc ; Jockenhoevel, Stefan, MD, PhD ; Mela, Petra, PhD ; Jashari, Ramadan, MD, PhD ; Gewillig, Marc, MD, PhD ; Hoylaerts, Marc F., PhD ; Meyns, Bart, MD, PhD ; Heying, Ruth, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-4c642a32bb3c63a037cbc981c02f0a07089a78571e958bc41a42760fa0c153d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cardiothoracic Surgery</topic><topic>congenital heart disease</topic><topic>congenital heart disease surgery</topic><topic>endocarditis</topic><topic>prosthetic heart valve</topic><topic>valved conduit</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Veloso, Tiago Rafael, PhD</creatorcontrib><creatorcontrib>Claes, Jorien, PhD</creatorcontrib><creatorcontrib>Van kerckhoven, Soetkin</creatorcontrib><creatorcontrib>Ditkowski, Bartosz, PhD</creatorcontrib><creatorcontrib>Hurtado-Aguilar, Luis G., MSc</creatorcontrib><creatorcontrib>Jockenhoevel, Stefan, MD, PhD</creatorcontrib><creatorcontrib>Mela, Petra, PhD</creatorcontrib><creatorcontrib>Jashari, Ramadan, MD, PhD</creatorcontrib><creatorcontrib>Gewillig, Marc, MD, PhD</creatorcontrib><creatorcontrib>Hoylaerts, Marc F., PhD</creatorcontrib><creatorcontrib>Meyns, Bart, MD, PhD</creatorcontrib><creatorcontrib>Heying, Ruth, MD, PhD</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Veloso, Tiago Rafael, PhD</au><au>Claes, Jorien, PhD</au><au>Van kerckhoven, Soetkin</au><au>Ditkowski, Bartosz, PhD</au><au>Hurtado-Aguilar, Luis G., MSc</au><au>Jockenhoevel, Stefan, MD, PhD</au><au>Mela, Petra, PhD</au><au>Jashari, Ramadan, MD, PhD</au><au>Gewillig, Marc, MD, PhD</au><au>Hoylaerts, Marc F., PhD</au><au>Meyns, Bart, MD, PhD</au><au>Heying, Ruth, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial Adherence to Graft Tissues in Static and Flow Conditions</atitle><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle><addtitle>J Thorac Cardiovasc Surg</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>155</volume><issue>1</issue><spage>325</spage><epage>332.e4</epage><pages>325-332.e4</pages><issn>0022-5223</issn><eissn>1097-685X</eissn><abstract>Abstract Objective Various conduits or stent-mounted valves are used as pulmonary valve graft tissues for Right Ventricular Outflow Tract reconstruction with good hemodynamic results. Valve replacement inherits an increased risk of infective endocarditis (IE). Recent observations rose awareness to IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. The present study focuses on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE. Methods Staphylococcus aureus , S. epidermidis and Streptococcus sanguinis adhesion to bovine pericardium (BP) patch, bovine jugular vein (BJV) and cryopreserved homograft (CH) tissues was quantified under static and shear stress conditions. Microscopic analysis and histology were performed to evaluate bacterial adhesion to matrix components. Results In general, similar bacteria numbers were recovered from CH and BJV tissue surface for all strains, especially in flow conditions. Static bacterial adhesion to CH wall was lower for S. sanguinis adhesion ( P <0.05 vs. BP patch). Adhesion to BJV wall, CH wall and leaflet were found to be decreased for S. epidermidis in static conditions ( P <0.05 vs. BP patch). Bacterial adhesion under shear stress indicates a similar bacterial adhesion to all tissues, except for a decrease to BJV wall after S. sanguinis incubation. Microscopic analysis shows the importance of matrix component exposure for bacterial adherence to CH. Conclusions Our data exhibit evidence that the surface composition of BJV and CH tissues itself, bacterial surface proteins or shear forces per se are not the prime determinants during initial bacterial adherence.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28712577</pmid><doi>10.1016/j.jtcvs.2017.06.014</doi><oa>free_for_read</oa></addata></record> |
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| source | Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Cardiothoracic Surgery congenital heart disease congenital heart disease surgery endocarditis prosthetic heart valve valved conduit |
| title | Bacterial Adherence to Graft Tissues in Static and Flow Conditions |
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