Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

Sample preparation for scanning electron microscope analysis involves reagents and equipment that are expensive and often hazardous. Here we demonstrate a circumvention of Osmium tetroxide and critical point drying, greatly reducing the duration, complexity and cost of the process. We captured early...

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Veröffentlicht in:	Analytical biochemistry 2017-10, Vol.534, p.46-48
Hauptverfasser:	Osahor, Andrew, Deekonda, Karthik, Lee, Choon-Weng, Sim, Edmund Ui-Hang, Radu, Aurelian, Narayanan, Kumaran
Format:	Artikel
Sprache:	eng
Schlagworte:	Cells Dehydration DNA Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Gene Transfer Techniques Genetic Vectors - genetics HeLa HeLa Cells Humans Microscopy osmium tetroxide Microscopy, Electron, Scanning Osmium Tetroxide - chemistry
Online-Zugang:	Volltext
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